Molecular Insights into TT2-Type MYB Regulators Illuminate the Complexity of Floral Flavonoids Biosynthesis in Freesia hybrida DOI Creative Commons
Xiaotong Shan,

Deyu Zhuang,

Ruifang Gao

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

Proanthocyanidins (PAs), anthocyanins, and flavonols are key flavonoids that play diverse roles in plant physiology human health. Despite originating from a shared biosynthetic pathway, the regulatory mechanisms of PA biosynthesis cooperative regulation different kinds remain elusive, particularly flower tissues or organs. Here, we elucidated network governing Freesia hybrida 'Red River®' by characterizing four TT2-type MYB transcription factors, designated FhMYBPAs. Phylogenetic analysis, subcellular localization, transactivation assays predicted their as PA-related activators. Pearson correlation analysis revealed significant correlations between FhMYBPAs accumulation various floral development stages. Functional studies demonstrated activated directly binding to promoters target genes, which can be enhanced FhTT8L. Additionally, hierarchical feedback model involving FhTTG1, FhMYB27, FhMYBx was proposed for biosynthesis. Furthermore, comparative flavonoid-related factors FhPAP1, FhMYB5, FhMYBF1, FhMYB21L2 highlighted regulating PA, anthocyanin, flavonol biosynthesis, with some exhibiting versatile regulations. Overall, our findings provide insights into spatio-temporal flowers expand understanding MYB-mediated transcriptional specialized metabolites plants.

Language: Английский

Targeted metabolite and molecular profiling of carotenoids in rose petals: A step forward towards functional food applications DOI
Bixuan Cheng, Xinying Liu, Yuchen Liu

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 464, P. 141675 - 141675

Published: Oct. 16, 2024

Language: Английский

Citations

0
Molecular Insights into TT2-Type MYB Regulators Illuminate the Complexity of Floral Flavonoids Biosynthesis in Freesia hybrida DOI Creative Commons
Xiaotong Shan,

Deyu Zhuang,

Ruifang Gao

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

Proanthocyanidins (PAs), anthocyanins, and flavonols are key flavonoids that play diverse roles in plant physiology human health. Despite originating from a shared biosynthetic pathway, the regulatory mechanisms of PA biosynthesis cooperative regulation different kinds remain elusive, particularly flower tissues or organs. Here, we elucidated network governing Freesia hybrida 'Red River®' by characterizing four TT2-type MYB transcription factors, designated FhMYBPAs. Phylogenetic analysis, subcellular localization, transactivation assays predicted their as PA-related activators. Pearson correlation analysis revealed significant correlations between FhMYBPAs accumulation various floral development stages. Functional studies demonstrated activated directly binding to promoters target genes, which can be enhanced FhTT8L. Additionally, hierarchical feedback model involving FhTTG1, FhMYB27, FhMYBx was proposed for biosynthesis. Furthermore, comparative flavonoid-related factors FhPAP1, FhMYB5, FhMYBF1, FhMYB21L2 highlighted regulating PA, anthocyanin, flavonol biosynthesis, with some exhibiting versatile regulations. Overall, our findings provide insights into spatio-temporal flowers expand understanding MYB-mediated transcriptional specialized metabolites plants.

Language: Английский

Citations

0