Sucrose transport gene FaSWEET9a regulated by FaDOF2 transcription factor promotes sucrose accumulation in strawberry DOI
Yan Xu, Shuang Liu, Hongying Sun

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Abstract This study identified and characterized 25 members of the SWEET gene family in genome cultivated strawberry (Fragaria × ananassa cv. ‘Yanli’), focusing on their potential roles fruit development. Notably, FaSWEET9a, a specific member SWEET family, was found to be uniquely expressed ‘Yanli’ fruit. Functional analysis via heterologous expression Saccharomyces cerevisiae confirmed that FaSWEET9a acts as sucrose transporter. To further investigate its role, we generated FaSWEET9a overexpression lines demonstrated FaSWEET9anot only enhances accumulation fruits but also influences plant growth We FaDOF2 could bind promoter enhance transcription by conducting yeast one-hybrid assays, electrophoretic mobility shift β-glucuronidase luciferase reporter assays. Moreover, transient transformation experiments revealed elevate content regulating FaSWEET9a. research brings new viewpoints molecular mechanisms govern regulation fruits, spotlighting functional significance the FaSWEET9a-FaDOF2 regulatory module aspects quality

Language: Английский

Sucrose transport gene FaSWEET9a regulated by FaDOF2 transcription factor promotes sucrose accumulation in strawberry DOI
Yan Xu, Shuang Liu, Hongying Sun

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Abstract This study identified and characterized 25 members of the SWEET gene family in genome cultivated strawberry (Fragaria × ananassa cv. ‘Yanli’), focusing on their potential roles fruit development. Notably, FaSWEET9a, a specific member SWEET family, was found to be uniquely expressed ‘Yanli’ fruit. Functional analysis via heterologous expression Saccharomyces cerevisiae confirmed that FaSWEET9a acts as sucrose transporter. To further investigate its role, we generated FaSWEET9a overexpression lines demonstrated FaSWEET9anot only enhances accumulation fruits but also influences plant growth We FaDOF2 could bind promoter enhance transcription by conducting yeast one-hybrid assays, electrophoretic mobility shift β-glucuronidase luciferase reporter assays. Moreover, transient transformation experiments revealed elevate content regulating FaSWEET9a. research brings new viewpoints molecular mechanisms govern regulation fruits, spotlighting functional significance the FaSWEET9a-FaDOF2 regulatory module aspects quality

Language: Английский

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