Development of Multiplex RT qPCR Assays for Simultaneous Detection and Quantification of Faecal Indicator Bacteria in Bathing Recreational Waters

Microorganisms, Journal Year: 2024, Volume and Issue: 12(6), P. 1223 - 1223

Published: June 18, 2024

In this study, we designed and validated in silico experimentally a rapid, sensitive, specific multiplex RT qPCR for the detection quantification of faecal indicator bacteria (FIB) used as microbiological references marine bathing water regulations (Escherichia coli intestinal enterococci). The 16S rRNA gene was to quantify group-specific enterococci Escherichia/Shigella species-specific such Enterococcus faecalis E. faecium. Additionally, ybbW encoding allantoin transporter protein detect coli. An assessment coastal systems (i.e., sediment) revealed that were predominant group compared Escherichia/Shigella. low contribution reported. As faecium reported at concentrations, it is assumed other origin are contributing high copy number enterococci. Moreover, numbers with respect seawater sediment. We conclude sediments can affect FIBs included regulations. Valuing quality system through sediment monitoring recommended.

Language: Английский

Substantial growth of Health Sciences Review

Health Sciences Review, Journal Year: 2024, Volume and Issue: 11, P. 100182 - 100182

Published: June 1, 2024

Language: Английский