Exploring biodegradation limits of n-alkanes as polyethylene models using multi-omics approaches

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 977, P. 179365 - 179365

Published: April 14, 2025

Polyethylene (PE) is widely regarded as non-biodegradable in natural environments, despite reports suggesting partial biotic degradation. Using multi-omics analysis, this study investigated the biodegradation mechanisms of n-alkanes-structural analogs PE-to determine threshold carbon number PE that allows for environmental biodegradation. n-Alkanes with 6-40 carbons (C6-C40) were biodegraded soil, whereas C44 and not. 16S rRNA gene amplicon sequence analysis identified distinct microbial communities associated non-degradable compounds (PEs C44) biodegradable alkanes (C6-C40). Notably, community composition C40 differed from those below C36. Multi-omics genera Aeromicrobium, Nocardia, Nocardioides, Rhodococcus, Acinetobacter, Fontimonas key degraders n-alkanes at C36 below, utilizing alkane hydroxylases such monooxygenase (AlkB), LC-alkane Acinetobacter (AlmA), cytochrome P450 (CYP153). Conversely, was facilitated by taxa, including order Acidimicrobiales genera, Acidovorax, Sphingorhabdus, Prosthecobacter, Roseimicrobium using AlmA CYP153-type hydroxylases. This difference may explain reduced biodegradability above C40, PE.

Language: Английский

Assessment of Petroleum Hydrocarbons Biodegradation by Indigenous Bacillus Strains from Khyber Pakhtunkhwa, Pakistan

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: 205, P. 107728 - 107728

Published: May 20, 2025

Language: Английский

Degradation behaviors of asphalt by microorganisms in asphalt pavement structure

Bioresource Technology, Journal Year: 2024, Volume and Issue: 416, P. 131793 - 131793

Published: Nov. 9, 2024

Language: Английский