A dual-target molecular mechanism of pyrethrum repellency against mosquitoes

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: May 5, 2021

Abstract Pyrethrum extracts from flower heads of Chrysanthemum spp. have been used worldwide in insecticides and repellents. While the molecular mechanisms its insecticidal action are known, basis pyrethrum repellency remains a mystery. In this study, we find that principal components pyrethrum, pyrethrins, minor component, (E)-β-farnesene (EBF), each activate specific type olfactory receptor neurons Aedes aegypti mosquitoes. We identify Ae. odorant 31 (AaOr31) as cognate Or for EBF Or31-mediated is significantly synergized by pyrethrin-induced activation voltage-gated sodium channels. Thus, exerts spatial through novel, dual-target mechanism. Elucidation two-target mechanism may potential implications design development new generation synthetic repellents against major mosquito vectors infectious diseases.

Language: Английский

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Human attractive cues and mosquito host-seeking behavior

Trends in Parasitology, Journal Year: 2021, Volume and Issue: 38(3), P. 246 - 264

Published: Oct. 19, 2021

Language: Английский

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Olfactory systems across mosquito species

Cell and Tissue Research, Journal Year: 2021, Volume and Issue: 383(1), P. 75 - 90

Published: Jan. 1, 2021

There are 3559 species of mosquitoes in the world (Harbach 2018) but, so far, only a handful them have been focus olfactory neuroscience and neurobiology research. Here we discuss mosquito anatomy function connect these to ecology. We highlight least well-known thus most interesting aspects systems promising future directions. hope this review will encourage insect community work more broadly across instead focusing narrowly on main disease vectors.

Language: Английский

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Olfaction inAnophelesmosquitoes

Chemical Senses, Journal Year: 2021, Volume and Issue: 46

Published: Jan. 1, 2021

Abstract As vectors of disease, mosquitoes are a global threat to human health. The Anopheles mosquito is the deadliest species as insect vector malaria-causing parasite, which kills hundreds thousands every year. These reliant on their sense smell (olfaction) guide most behaviors, and better understanding olfaction identifies opportunities for reducing spread malaria. This review takes detailed look at olfaction. We explore range topics from chemosensory receptors, olfactory neurons, sensory appendages behaviors guided by (including host-seeking, foraging, oviposition, mating), management strategies that target identify many research areas remain be addressed.

Language: Английский

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Mutagenesis of the odorant receptor co-receptor (Orco) reveals severe olfactory defects in the crop pest moth Helicoverpa armigera

BMC Biology, Journal Year: 2022, Volume and Issue: 20(1)

Published: Sept. 30, 2022

Abstract Background Odorant receptors (ORs) as odorant-gated ion channels play a crucial role in insect olfaction. They are formed by heteromultimeric complex of the odorant receptor co-receptor (Orco) and ligand-selective Or. Other types olfactory proteins, such ionotropic (IRs) some gustatory (GRs), also involved system insects. Orco an obligatory subunit ORs is highly conserved, providing opportunity to systematically evaluate OR-dependent responses. Results Herein, we successfully established homozygous mutant ( −/− ) Helicoverpa armigera , notorious crop pest, using CRISPR/Cas9 gene editing technique. We then compared response characteristics wild type (WT) adults larvae. males were infertile, while females fertile. The lifespan was longer than that WT females. expressions most Ors Irs other olfaction-related genes adult antennae moths not obviously affected, but them up- or down-regulated. In addition, there no change neuroanatomical phenotype at level antennal lobe (including macroglomerular region male). Using EAG SSR techniques, discovered electrophysiological responses sex pheromone components many host plant odorants absent. upwind flight behaviors toward pheromones severely reduced wind tunnel experiment. oviposition selectivity (green pepper) has completely disappeared, chemotaxis green pepper lost Conclusions Our study indicates OR-mediated olfaction essential for communication, selection, larval H. suggesting strategy which mate searching host-seeking moth pests could be disrupted inhibiting silencing expression.

Language: Английский

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Discrete roles of Ir76b ionotropic coreceptor impact olfaction, blood feeding, and mating in the malaria vector mosquito Anopheles coluzzii

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(23)

Published: June 1, 2022

Significance Chemosensory systems play crucial roles across mosquito life cycles, with underlying molecular receptors often forming functional complexes that require cognate coreceptors. To better understand chemosensory pathways in the malaria vector Anopheles coluzzii , gene editing were used to localize and functionally characterize ionotropic receptor coreceptor AcIr76b . Expression of was observed antennal grooved pegs, coeloconic sensilla, other accessory olfactory appendages. When inactivated, mutant mosquitoes displayed altered neuronal responses amines.. Beyond phenotypes, mutants display significantly impaired mating blood feeding capabilities. Our data reveal discrete pathways, suggesting as targets for design control strategies.

Language: Английский

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Identification of human skin microbiome odorants that manipulate mosquito landing behavior

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 18, 2024

Abstract The resident human skin microbiome is responsible for the production of most scents that are attractive to mosquitoes. Hence, engineering synthesize less mosquito attractants or produce repellents could potentially reduce bites and prevent transmission deadly mosquito-borne pathogens. In order further characterize volatilome, we quantified major volatiles 39 strains commensals ( Staphylococci Corynebacterium ). Importantly, validate behavioral activity these volatiles, first assessed landing behavior triggered by volatiles. We demonstrated gated presence carbon dioxide L-(+)-lactic acid. This similar combinatorial coding triggering short range attraction. Repellency selected terpenes was tested in a 2-choice context, 2- 3-methyl butyric acids reduced 62.0–81.6% 87.1–99.6%, respectively. Similarly, terpene geraniol capable reducing 74.9%. also potential repellency effects mosquitoes at short-range using 4-port olfactometer. assays, attraction (69–78%) mixture key kairomones dioxide, acid, ammonia. These findings demonstrate acid change valence other towards behavior. Moreover, this study offers candidate odorants be targeted novel strategy microbiota may curtail bites, subsequent disease.

Language: Английский

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Genome editing in pests: basic science to applications

Journal of Pest Science, Journal Year: 2024, Volume and Issue: 97(3), P. 1135 - 1152

Published: Jan. 24, 2024

Language: Английский

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Neurodevelopmental and transcriptomic effects of CRISPR/Cas9-induced somatic orco mutation in honey bees

Journal of Neurogenetics, Journal Year: 2021, Volume and Issue: 35(3), P. 320 - 332

Published: March 5, 2021

In insects, odorant receptors facilitate olfactory communication and require the functionality of highly conserved co-receptor gene orco. Genome editing studies in a few species ants moths have revealed that orco can also neurodevelopmental function, addition to its canonical role adult olfaction, discovered first Drosophila melanogaster. To extend this analysis, we determined whether mutations affect development brain honey bee Apis mellifera, an important model system for social behavior chemical communication. We used CRISPR/Cas9 knock out examined anatomical molecular consequences. increase efficiency, coupled embryo microinjection with laboratory egg collection vitro rearing system. This new workflow advances genomic engineering technologies bees by overcoming restrictions associated field studies. Sanger sequencing quickly select individuals complete knockout neuroanatomical analyses later validated described amplicon sequencing. Mutant had significantly fewer glomeruli, smaller total volume all higher mean individual glomerulus antennal lobe compared wild-type controls. RNA-Sequencing caused differential expression hundreds genes antenna, including related neural encoding receptors. The other types chemoreceptor was generally unaffected, reflecting specificity CRISPR activity study. These results suggest effects are specific insect life histories.

Language: Английский

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Recent Insights into Insect Olfactory Receptors and Odorant-Binding Proteins

Insects, Journal Year: 2022, Volume and Issue: 13(10), P. 926 - 926

Published: Oct. 13, 2022

Human and insect olfaction share many general features, but insects differ from mammalian systems in important ways. Mammalian olfactory neurons the same overlying fluid layer nose, neuronal tuning entirely depends upon receptor specificity. In insects, are anatomically segregated into sensilla, small clusters of dendrites extracellular that can be independently regulated different sensilla. Small proteins called odorant-binding differentially secreted this sensillum lymph where they have been shown to confer sensitivity specific odorants, also affect kinetics neuron responses. Insect receptors not G-protein-coupled receptors, such as vertebrate ligand-gated ion channels opened by direct interactions with odorant molecules. Recently, several examples expressing multiple identified, indicating mechanisms for may broader than mammals. Finally, recent advances genome editing finding applications species, including agricultural pests human disease vectors.

Language: Английский

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