Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus DOI Creative Commons
Jimena Leyria, Ian Orchard, Angela B. Lange

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(7), P. e0306611 - e0306611

Published: July 12, 2024

In insects, biogenic amines function as neurotransmitters, neuromodulators, and neurohormones, influencing various behaviors, including those related to reproduction such response sex pheromones, oogenesis, oviposition, courtship, mating. Octopamine (OA), an analog of the vertebrate norepinephrine, is synthesized from amine tyramine by enzyme β-hydroxylase (TβH). Here, we investigate mechanisms target genes underlying role OA in successful females Rhodnius prolixus , a vector Chagas disease, downregulating TβH mRNA expression (thereby reducing content) using RNA interference (RNAi), vivo ex application OA. Injection with dsTβH impairs at least part, decreasing transcript enzymes involved juvenile hormone biosynthesis, primary for oogenesis R . thereby interfering ovulation oviposition. This study offers valuable insights into involvement females. Understanding reproductive biology crucial medical context controlling spread disease.

Language: Английский

Endocrine factors modulating vitellogenesis and oogenesis in insects: An update DOI
Jimena Leyria

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 587, P. 112211 - 112211

Published: March 15, 2024

Language: Английский

Citations

13
A tyramine receptor gene LsTAR2 is involved in reproduction and feeding in the small brown planthopper Laodelphax striatellus DOI
Gang Xu, Liran Fu, Liang Wu

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 209, P. 106335 - 106335

Published: Feb. 18, 2025

Language: Английский

Citations

1
Combined analysis of the proteome and metabolome provides insight into microRNA-1174 function in Aedes aegypti mosquitoes DOI Creative Commons

Yangrui Luo,

Dun Liu, Yuanmei Wang

et al.

Parasites & Vectors, Journal Year: 2023, Volume and Issue: 16(1)

Published: Aug. 9, 2023

Pathogenic viruses can be transmitted by female Aedes aegypti (Ae. aegypti) mosquitoes during blood-meal acquisition from vertebrates. Silencing of mosquito- and midgut-specific microRNA (miRNA) 1174 (miR-1174) impairs blood intake increases mortality. Determining the identity proteins metabolites that respond to miR-1174 depletion will increase our understanding molecular mechanisms this miRNA in controlling blood-feeding nutrient metabolism mosquitoes. Antisense oligonucleotides (antagomirs [Ant]) Ant-1174 Ant-Ct were injected into Ae. at 12-20 h posteclosion, was confirmed reverse transcription quantitative real-time PCR (RT-qPCR). Ant-1174-injected control collected before meal 72 post-injection for tandem mass tag-based proteomic analysis liquid chromatography-tandom spectrometry non-target metabolomic identify differentially expressed metabolites, respectively. RNA interference (RNAi) using double-stranded (dsRNA) injection applied investigate biological roles these genes. The RNAi effect verified RT-qPCR western blotting assays. Triglyceride content ATP levels measured appropriate assay kits, following manufacturers' instructions. Statistical analyses conducted with GraphPad7 software Student's t-test. Upon miR-1174, a total 383 (DEPs) identified, among which 258 upregulated 125 downregulated. Functional DEPs Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment suggested plays important regulatory amino acid metabolism, nucleotide fatty sugar pathways. A 292 differential 141 151 Integrative showed associated mainly enriched variety metabolic pathways, including glycolysis, citrate cycle, oxidative phosphorylation metabolism. Specifically, gene one protein miR-1174-depleted mosquitoes, purine nucleoside phosphorylase (PNP; AAEL002269), purine, pyrimidine niacin-nicotinamide PNP knockdown seriously inhibited digestion ovary development increased adult Mechanically, led significant downregulation vitellogenin (Vg); addition, some genes ecdysone signaling insulin-like peptide pathways related affected. This study demonstrates accumulation techniques. results provide functional evidence role gut physiological activities. Our findings highlight key changes thus basis novel insights mechanism involved lineage-specific mosquito vectors.

Language: Английский

Citations

8
The octopamine receptor OAα1 influences oogenesis and reproductive performance in Rhodnius prolixus DOI Creative Commons
Luca Finetti, Ian Orchard, Angela B. Lange

et al.

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(12), P. e0296463 - e0296463

Published: Dec. 29, 2023

The control of reproductive processes in Rhodnius prolixus involves a variety neuroactive chemicals. Among these, several studies have suggested that the biogenic amine octopamine (OA), might play an active role these processes. Here, we investigate molecular profile R . α adrenergic-like OA receptor 1 (RpOAα1-R) and its egg production. Comparative analyses confirm RpOAα1-R gene codes for true OAα1 receptor. transcript is highly expressed tissues associated with production, after blood meal, which stimulus full production , upregulated ovaries spermatheca. After RNAi-mediated knockdown, ovarian phenotype characterized by slow development observed. Furthermore, altered has been eggs are deformed. Interestingly, there no evidence disruption vitellogenin (Vg) synthesis fat body or uptake oocytes. On other hand, downregulation correlated defective choriogenesis eggs. These results provide critical information concerning OAα1-R oogenesis

Language: Английский

Citations

8
Functional analyses of dopamine receptors involved in virus transmission and reproduction in the small brown planthopper Laodelphax striatellus DOI
Gang Xu, Qiuxin Zhang,

Mingshi Qian

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 205, P. 106157 - 106157

Published: Oct. 1, 2024

Language: Английский

Citations

2
Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus DOI Creative Commons
Jimena Leyria, Ian Orchard, Angela B. Lange

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(7), P. e0306611 - e0306611

Published: July 12, 2024

In insects, biogenic amines function as neurotransmitters, neuromodulators, and neurohormones, influencing various behaviors, including those related to reproduction such response sex pheromones, oogenesis, oviposition, courtship, mating. Octopamine (OA), an analog of the vertebrate norepinephrine, is synthesized from amine tyramine by enzyme β-hydroxylase (TβH). Here, we investigate mechanisms target genes underlying role OA in successful females Rhodnius prolixus , a vector Chagas disease, downregulating TβH mRNA expression (thereby reducing content) using RNA interference (RNAi), vivo ex application OA. Injection with dsTβH impairs at least part, decreasing transcript enzymes involved juvenile hormone biosynthesis, primary for oogenesis R . thereby interfering ovulation oviposition. This study offers valuable insights into involvement females. Understanding reproductive biology crucial medical context controlling spread disease.

Language: Английский

Citations

0