Identification and functional characterization of CYP3002B2, a cytochrome P450 associated with amitraz and flumethrin resistance in the major bee parasite Varroa destructor

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 210, P. 106364 - 106364

Published: March 4, 2025

Beekeeping worldwide is increasingly threatened by the parasitic mite Varroa destructor, whose management relies heavily on synthetic acaricides such as amitraz and flumethrin. However, growing incidence of acaricide resistance in V. destructor presents a significant global challenge to apiculture. In this study, we investigated mechanisms underlying these compounds population exhibiting reduced susceptibility both Specifically, bioassays revealed that resistant (IL-R) displayed 35.0 % mortality response 39.5 flumethrin, contrast >90 observed susceptible IL-L ATH-S populations. The phenotype was not strongly associated with any known target site mutations; putative mutation F290L Octβ2R gene, pyrethroid L925V vgsc were found at low frequencies (8.6 13.6 respectively). Transcriptomic analysis, comparing gene expression levels between two populations, overexpression several cuticle genes cytochrome P450 CYP3002B2. CYP3002B2 functionally expressed E. coli, catalytic activity against multiple model substrates effectively metabolizing predominant product metabolism likely an inactive, hydroxylated form insecticide, rather than activated/toxic metabolites amitraz. These findings are crucial for evidence-based management, first detoxification enzyme shown metabolize major from different modes action classes.

Language: Английский

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Study on the variations in acaricide sensitivity between two spider mite species, Amphitetranychus viennensis and Tetranychus urticae, in Chinese orchards

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 210, P. 106367 - 106367

Published: March 4, 2025

Language: Английский

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Acaricide Resistance Monitoring and Structural Insights for Precision Tetranychus urticae Management

Insects, Journal Year: 2025, Volume and Issue: 16(5), P. 440 - 440

Published: April 23, 2025

The two-spotted spider mite (Tetranychus urticae) is a highly destructive and economically significant pest in agricultural, horticultural, ornamental agroecosystems worldwide, including hop (Humulus lupulus) mint (Mentha spp.) fields the Pacific Northwest (PNW) region of United States. Repeated acaricide applications rotations have led to widespread resistance, resulting control failures. In this study, we investigated mechanisms resistance four different acaricides (bifenthrin, bifenazate, etoxazole, abamectin) across 23 field-collected TSSM populations by integrating diagnostic bioassays, genetic screening for resistance-associated mutations, structural modeling, molecular docking. Several kdr mutations mutation combinations were detected TuVGSC all tested populations. G132A Tucytb was identified 68.75% 40% populations, while I1017F TuCHS 1 found 94% 100% Structural analysis revealed key interactions between target proteins both wild-type mutant variants, providing novel insights into functional impacts these mutations. Our findings enhance understanding adaptation among crops, supporting development more effective management strategies mitigate economic losses hops, mint, other crop production.

Language: Английский

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Genome sequences of fourIxodesspecies expands understanding of tick evolution

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 29, 2024

Abstract Ticks, hematophagous acari, pose a significant threat by transmitting various pathogens to their vertebrate hosts during feeding. Despite advances in tick genomics, high-quality genomes were lacking until recently, particularly the genus Ixodes , which includes main vectors of Lyme disease. Here, we present complete genome sequences four species, derived from single female individual, with particular focus on European species ricinus achieving chromosome-level assembly. Additionally, draft assemblies generated for three other I. persulcatus, pacificus and hexagonus . The quality extensive annotation several important gene families have allowed us study evolution repertoires at level group. We determined that undergone major amplifications ticks, while an expression atlas obtained reveals striking patterns specialization both between within families. Notably, family are associated proliferation single-exon genes. integration our data existing establishes solid framework evolution, improving understanding biology. In addition, work lays foundations applied research innovative control targeting these organisms.

Language: Английский

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Overexpression of a nuclear receptor HR96 contributes to spirodiclofen susceptibility in Panonychus citri (McGregor)

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 202, P. 105952 - 105952

Published: May 11, 2024

Language: Английский

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