Naturally-Occurring Sulphur Compounds

Published: Jan. 1, 2025

Language: Английский

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Functional Analysis of Lactase Phlorizin Hydrolase in Insect–Plant Coevolution Based on Deglycosylation

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: 73(9), P. 5140 - 5149

Published: Feb. 24, 2025

During coevolution, plants produce numerous toxic secondary metabolites to protect themselves. However, most still serve as food for insects meet their nutritional needs. The evolutionary processes that enable herbivorous resist plant defenses remain largely complex and difficult predict. In this study, lactase phlorizin hydrolase (LPH) was identified the first time in Aphis gossypii. Bioinformatics analysis showed LPH protein belongs glycoside 1 (GH1) family. qPCR transcript level of cotton aphids treated with expression dsAgCYP6CY3 lines significantly reduced by 39.9% at 48 h compared nontransgenic cotton, hydrolytic activities on lactose were 28.7% 20.1%, respectively. vitro enzyme activity experiments demonstrated could hydrolyze four phenolic glycosides. addition, RNA interference (RNAi) insect performance assays silencing affected growth larvae, resulting death larvae exposed These results reveal an scenario whereby herbivores use deglycosylation develop resistance can be exploited protection.

Language: Английский

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Polygenic response to selection by transgenic Bt-expressing crops in wild Helicoverpa zea and characterization of a major effect locus

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Dec. 26, 2024

Strong and shifting selective pressures of the Anthropocene are rapidly shaping phenomes genomes organisms worldwide. Crops expressing pesticidal proteins from Bacillus thuringiensis (Bt) represent one major force on insect genomes. Here we characterize a rapid response to selection by Bt crops in crop pest, Helicoverpa zea. We reveal polygenic architecture resistance evolution H. zea identify multiple genomic regions underlying this trait. In region largest effect, identified gene amplification event, where resistant individuals showed variation copy number for genes. Signals increased over time, consistent with history field-evolved evolution. Modern wild populations disparate geographical positive variant at high, but not fixed, allele frequencies. also detected against single variants locus collected plants, further supporting its role resistance. Multiple genes were annotated region, all appeared be significantly upregulated functionally characterized within (CNV), providing insight into their potential roles Our findings nature genome pest following anthropogenic highlight that CNVs can have evolutionary responses.

Language: Английский

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The polyvalent sequestration ability of an economically important beetle

Current Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

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A bioinformatic examination of indole-3-acetic acid biosynthesis in insecta and hexapoda

Arthropod-Plant Interactions, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 28, 2024

Language: Английский

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