Enhanced silk production and pupal weight in Bombyx mori through CRISPR/Cas9-mediated circadian Clock gene disruption

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0317572 - e0317572

Published: Jan. 27, 2025

The domesticated silkworm, Bombyx mori , is crucial for global silk production, which a significant economic activity supporting millions of livelihoods worldwide. Beyond traditional the growing demand insect larvae in cosmetics, biomedical products, and animal feed underscores need to enhance B . productivity. This study investigates role circadian clock gene Clock using CRISPR/Cas9-mediated mutagenesis establish Clk Δ29 knock-out mutant strain. Dysregulation was demonstrated by altered temporal transcriptional profiles core genes adult heads disrupted circadian-controlled behaviors, including eclosion egg hatching rhythms under constant darkness. By analysing larval development timing, as well weights late instar larvae, pupae, cocoon components mutants Δ1922 silkworms (carrying an independently generated - null allele), we showed that CLK contributes physiological processes regulating growth. Importantly, reared on standard sericulture diet exhibited increases key traits, with production increasing up 7%, pupal weight 25% compared wild-type controls. highlights potential manipulation significantly sericultural Future research should focus elucidating molecular mechanisms driving these phenotypes determining whether they result from functions or pleiotropic effects These findings provide foundation advancing sustainable developing new commercial applications silkworm-derived products.

Language: Английский

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Evolution of circadian clock and light-input pathway genes in Hemiptera

Insect Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 104298 - 104298

Published: March 1, 2025

Language: Английский

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Distinct timing systems operate the locomotor activity rhythm and the photoperiodic response in the parasitic jewel wasp, Nasonia vitripennis

Biological Rhythm Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 11

Published: April 9, 2025

Language: Английский

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Photoperiodism, Insects

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

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