Synthesis of chitosan/PVA/copper oxide nanocomposite using Anacardium occidentale extract and evaluating its antioxidant, antibacterial, anti-inflammatory and cytotoxic activities

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 31, 2025

Nanotechnology has witnessed remarkable advancements in recent years, capturing considerable attention diverse biomedical applications. Using the green precipitation method, this study aims to synthesize and characterize chitosan/polyvinyl alcohol-copper oxide nanocomposites (CS/PVA/CuONCs) using Anacardium occidentale plant fruit extract. The CS/PVA/CuONCs were further evaluated antioxidant, antibacterial biological activities. In our results, UV-Vis spectrum analysis of revealed a peak at 430 nm. FTIR analyses confirmed presence different functional groups, while XRD crystalline structure synthesized nanocomposites. FESEM-EDAX demonstrated that exhibited spherical rod-like shape, with an average particle size 48.6 96.2 Notably, higher antioxidant activity, as evidenced by their ABTS activity (83.79 ± 1.57%) SOD (86.17 1.28%). assays, inhibition Escherichia coli 20.52 0.85 mm Bacillus subtilis 19.64 0.87 mm, displaying zone inhibition. excellent anti-inflammatory potency against COX-1 (67.10 0.58%) COX-2 (76.39 0.65%). antidiabetic assay α-amylase (80.25 1.29%) α-glucosidase (84.74 1.42%) Anti-cholinergic AChE was 65.35 0.98% BuChE 82.46 1.15% are observed. shown have strong cytotoxicity MCF-7 cell lines. It also had highest viability inhibition, 13.66 0.58%. hemolysis found be 5.38 0.34%. Overall, possess remarkably

Language: Английский

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Combinatorial Enzymatic Catalysis for Bioproduction of Ginsenoside Compound K

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(7), P. 3385 - 3397

Published: Feb. 13, 2023

Ginsenoside compound K (CK) is an emerging functional food or pharmaceutical product. To date, there are still challenges to exploring effective catalytic enzymes for enzyme-catalyzed manufacturing processes and establishing processes. Herein, we identified three ginsenoside hydrolases BG07 (glucoamylase), BG19 (β-glucosidase), BG23 (β-glucosidase) from Aspergillus tubingensis JE0609 by transcriptome analysis peptide mass fingerprinting. Among them, was expressed in Komagataella phaffii with a high volumetric activity of 235.73 U mL–1 (pNPG). Enzymatic property studies have shown that acidic (pH adaptation range 4.5–7.0) mesophilic (thermostable < 50 °C) enzyme. Moreover, one-pot combinatorial strategy based on BGA35 (β-galactosidase oryzae) established, CK yield 396.7 mg L–1 h–1. This study explored the derived A. at molecular level provided reference efficient production CK.

Language: Английский

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Molecular-level insight into the effects of low moisture and trehalose on the thermostability of β-glucosidase

Food Chemistry, Journal Year: 2024, Volume and Issue: 460, P. 140607 - 140607

Published: July 24, 2024

Language: Английский

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Metagenomics of the midgut microbiome of Rhipicephalus microplus from China

Parasites & Vectors, Journal Year: 2022, Volume and Issue: 15(1)

Published: Feb. 8, 2022

Abstract Background Ticks, which are ectoparasites of animals, may carry multiple pathogens. The cattle tick Rhipicephalus microplus is an important bovine parasite in China. However, the midgut microbiome R. from China has not been characterized via metagenomic methods. Methods were collected city Changsha Hunan province, DNA contents was extracted fully engorged adult female R . A library constructed and sequenced using Illumina HiSeq sequencing platform. SOAPdenovo software used to assemble analyze clean data. latent class analysis algorithm applied system classification by MEGAN annotate information on species’ sequences. DIAMOND compare unigenes with Kyoto Encyclopedia Genes Genomes (KEGG) database, functional annotation carried out based results comparison. Results dominant phyla five samples Firmicutes, Proteobacteria, Actinobacteria. Streptococcus , Mycobacterium Anaplasma Enterococcus Shigella Lactobacillus Brachyspira Pseudomonas Enterobacter Bacillus Lactococcus genera samples. endosymbiotic bacterium Wolbachia also detected all malmesburyense pneumoniae phagocytophilum faecium sonnei faecalis casei hampsonii syringae cloacae garvieae species In addition these bacterial species, we some eukaryotes, such as Rhizophagus irregularis Enterospora canceri Smittium culicis Zancudomyces culisetae Trachipleistophora hominis viruses orf virus, human endogenous retrovirus type W, enzootic nasal tumor virus goats, CH15, galidia at level. annotated KEGG pathway predictions for gene functions microflora indicated genes involved lipid amino acid metabolism, infectious diseases (e.g., pneumonia infection, granulocytic anaplasmosis, Salmonella enterica pathogenic Escherichia coli infection), cancer. Conclusions Our study revealed that only composed a large number bacteria, but portion comprises eukaryotes viruses. data presented here enhance our understanding this tick’s provide fundamental control ticks tick-borne diseases. Graphical

Language: Английский

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Biochemical and Structural Characterization of a Family-9 Glycoside Hydrolase bioprospected from the termite Syntermes wheeleri gut bacteria metagenome.

Enzyme and Microbial Technology, Journal Year: 2025, Volume and Issue: 189, P. 110654 - 110654

Published: April 16, 2025

Language: Английский

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Exploring termite-microbe symbiosis: mechanisms, evolution and biotechnological potential

Asia-Pacific Journal of Molecular Biology and Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 41 - 53

Published: April 10, 2025

The symbiotic relationship between termites and their gut microbes is one of nature's most fascinating mutualistic interactions. Termites, renowned for ability to decompose lignocellulosic materials such as wood, rely heavily on microbiota, which includes bacteria, archaea, protozoa, each playing specific roles in breaking down cellulose, hemicellulose, lignin. This complex partnership not only enables thrive low-nutrient environments but also supports nitrogen metabolism provides essential nutrients survival. termite highly compartmentalized, creating various microenvironments that support diverse microbial communities facilitate metabolic processes, including fermentation anoxic conditions. Shaped by evolutionary pressures, this has resulted specialized adaptations optimize lignocellulose degradation cycling. review explores the mechanisms termite-microbe symbiosis, functional across different taxa, structural compartmentalization gut, dynamics driving relationship. In addition, highlights ecological biotechnological significance offering valuable insights into efficient nutrient recycling could inspire future applications sustainable bioenergy waste management.

Language: Английский

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Seasonal stability of the rumen microbiome contributes to the adaptation patterns to extreme environmental conditions in grazing yak and cattle

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 23, 2024

Abstract Background The rumen microbiome plays an essential role in maintaining ruminants’ growth and performance even under extreme environmental conditions, however, which factors influence stability when ruminants are reared such habitats throughout the year is unclear. Hence, of yak (less domesticated) cattle (domesticated) on Qinghai-Tibetan Plateau through were assessed to evaluate temporal changes their composition, function, stability. Results Rumen fermentation characteristics pH significantly shifted across seasons both yak, but patterns differed between two ruminant species. Ruminal enzyme activity varied with season, production xylanase cellulase was greater compared fall winter. bacterial community season cattle, higher alpha diversity similarity (beta diversity) than cattle. indices eukaryotic did not change species, observed yak. In addition, functional seasons. Moreover, encoded more genes (GH2 GH3) related cellulose hemicellulose degradation a new family (GH160) gene involved oligosaccharides uniquely detected rumen. affected attenuation buffering values (stability), value Positive correlations antimicrobial resistance ( dfrF ) CAZyme (GH113) identified relationship negatively correlated Conclusions findings potential degradation, microbial abundance differently provide insight into mechanisms that may underpin divergent adaptation harsh climate Plateau. These results lay solid foundation for developing strategies maintain improve dig out candidates manufacturing lignocellulolytic enzymes enhance conditions.

Language: Английский

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Synthesis, nonlinear optical analysis and DFT studies of D–π–D and A–π–A configured Schiff bases derived from bis-phenylenediamine

RSC Advances, Journal Year: 2022, Volume and Issue: 12(50), P. 32185 - 32196

Published: Jan. 1, 2022

Herein, an integral approach has been made towards the exploration of electronic and structural parameters four synthesized (DMA with A-π-A configuration DMM, DAM, DMD a D-π-D configuration) one designed (DMB-D) novel Schiff base compounds. Bis phenylenediamine derivatives were prepared by condensation 4,5-dimethyl-o-phenylenediamine (1) various substituted benzaldehydes (2a-d). The structures compounds confirmed spectroscopic techniques, i.e., UV-visible, FT-IR, NMR spectroscopy. DFT-based analysis entitled was performed via density functional theory utilizing M06-2X in conjugation 6-311G(d,p) basis set to acquire geometrical parameters, natural bonding orbital (NBO), states (DOS), non-linear optical (NLO), molecular electrostatic potential (MESP), population analyses. smallest band gap (5.446 eV) noted for DMAvia frontier (FMO) analysis. GRPs obtained aid Egap values as DMA lowest displayed small magnitude hardness (2.723 large softness (0.183 eV). βtot DMA, DMB-D, 56.95, 0.43, 2.53, 8.98, 68.47 times larger than urea (βtot = 3.71 × 10-31 e.s.u.), respectively. observed fascinating NLO properties these might be helpful further advancement optics.

Language: Английский

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Improved the optical nonlinearity of carbazole based chromophores via molecular engineering: A DFT approach

Arabian Journal of Chemistry, Journal Year: 2024, Volume and Issue: 17(5), P. 105753 - 105753

Published: March 27, 2024

The present investigation was based on designing of carbazole derivatives (DTCFD1–DTCFD8) having D1-π-D2-π-A architecture from DTCFR, upon substituting acceptor motifs for nonlinear optical (NLO) materials. Density functional theory (DFT) analysis at M06-2X/6-311G(d,p) level accomplished to explore the optoelectronic behavior designed compounds. UV–Vis, density states (DOS), natural bond orbitals (NBOs), transition matrix (TDM) and frontier molecular (FMOs) analyses were executed comprehend properties above-mentioned FMO showed DTCFD7 as lowest energy gap 3.734 eV among derivatives, indicating its potential efficient charge transfer. overall decreasing order band gaps observed: DTCFR > DTCFD5 DTCFD3 DTCFD1 DTCFD2 DTCFD4 DTCFD6 DTCFD7. UV–Visible exploited that displayed distinctive absorption characteristics. In gas phase, it exhibited a λmax 494.927 nm, while in dichloromethane significantly higher 516.515 then all other derivatives. All greater values linear polarizability [〈α〉=1.633–1.786 × 10−22 esu], first hyperpolarizability (βtot = 3.690–6.549 10−28 esu) second (γtot 4.825–7.229 10−33 compared reference molecule. Furthermore, NLO revealed highest βtot (6.549 γtot (7.229 by DTCFD1, respectively, Moreover, comparative study with standard urea para-nitroaniline (p-NA), effective current light comprehensive findings, this concludes compounds may be considered

Language: Английский

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Recombinant GH3 β-glucosidase stimulated by xylose and tolerant to furfural and 5-hydroxymethylfurfural obtained from Aspergillus nidulans

Bioresources and Bioprocessing, Journal Year: 2024, Volume and Issue: 11(1)

Published: July 29, 2024

Abstract The β-glucosidase gene from Aspergillus nidulans FGSC A4 was cloned and overexpressed in the A. A773. resulting purified β-glucosidase, named AnGH3, is a monomeric enzyme with molecular weight of approximately 80 kDa, as confirmed by SDS-PAGE. Circular dichroism further validated its unique canonical barrel fold (β/α), feature also observed 3D homology model AnGH3. most striking aspect this recombinant robustness, it retained 100% activity after 24 h incubation at 45 50 ºC pH 6.0. Even 55 °C, maintained 72% enzymatic 6 same pH. kinetic parameters V max , K M Kcat/K for ρ -nitrophenyl-β-D-glucopyranoside ( NPG) cellobiose were determined. Using NPG, demonstrated 212 U mg − 1 0.0607 mmol L cat /K 4521 s when incubated 6.0 65 °C. using 2.7 57 27 –1 respectively. AnGH3 significantly enhanced xylose ethanol concentrations up to 1.5 mol 25%, challenging conditions, °C 6.0, activity, retaining 70% initial presence 200 furfural 5-hydroxymethylfurfural (HMF), potential application saccharification forage grass Panicum maximum where led 48% increase glucose release h. These characteristics, including high catalytic performance, good thermal stability hydrolysis temperature, tolerance elevated ethanol, D-xylose, furfural, HMF, position promising tool lignocellulosic biomass part an efficient multi-enzyme cocktail, thereby opening new avenues field biotechnology enzymology.

Language: Английский

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