The green tea catechin EGCG provides proof-of-concept for a pan-coronavirus attachment inhibitor DOI Creative Commons
Emmanuelle V. LeBlanc, Che C. Colpitts

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: July 28, 2022

Abstract The COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has emphasized serious threat to human health posed emerging coronaviruses. Effective broadly-acting antiviral countermeasures are urgently needed prepare for future CoVs, as vaccine development is not compatible with a rapid response newly virus. green tea catechin, epigallocatechin gallate (EGCG), broad-spectrum activity, although its mechanisms against coronavirus (CoV) infection have remained unclear. Here, we show that EGCG prevents and murine CoV blocks entry of lentiviral particles pseudotyped spike proteins from bat or highly pathogenic including SARS-CoV-2 variants concern, in lung epithelial cells. Mechanistically, treatment reduces attachment target cell surfaces interfering cell-surface glycans. Heparan sulfate proteoglycans required factor shown here be important endemic HCoV-OC43 infection. We can compete heparin, heparan analog, virion binding. Our results highlight conserved demonstrate potential pan-coronavirus inhibitors, which may useful protect CoVs.

Language: Английский

The SARS‐CoV‐2 main protease (Mpro): Structure, function, and emerging therapies for COVID‐19 DOI
Qing Hu, Yuan Xiong, Guanghao Zhu

et al.

MedComm, Journal Year: 2022, Volume and Issue: 3(3)

Published: July 14, 2022

The main proteases (M

Language: Английский

Citations

148
Therapeutic Effects of Green Tea Polyphenol (‒)-Epigallocatechin-3-Gallate (EGCG) in Relation to Molecular Pathways Controlling Inflammation, Oxidative Stress, and Apoptosis DOI Open Access
Daniela Mokrá, Marta Jošková, Juraj Mokrý

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 24(1), P. 340 - 340

Published: Dec. 25, 2022

(‒)-Epigallocatechin-3-gallate (EGCG) is the most abundant polyphenol in green tea. Thanks to multiple interactions with cell surface receptors, intracellular signaling pathways, and nuclear transcription factors, EGCG possesses a wide variety of anti-inflammatory, antioxidant, antifibrotic, anti-remodelation, tissue-protective properties which may be useful treatment various diseases, particularly cancer, neurological, cardiovascular, respiratory, metabolic disorders. This article reviews current information on biological effects above-mentioned disorders relation molecular pathways controlling inflammation, oxidative stress, apoptosis.

Language: Английский

Citations

120
Structure and function of SARS-CoV and SARS-CoV-2 main proteases and their inhibition: A comprehensive review DOI Creative Commons
Xin Li, Yongcheng Song

European Journal of Medicinal Chemistry, Journal Year: 2023, Volume and Issue: 260, P. 115772 - 115772

Published: Aug. 28, 2023

Language: Английский

Citations

52
Inhibition of the SARS-CoV-2 3CLpro main protease by plant polyphenols DOI Creative Commons
Miha Bahun, Marko Jukič, Domen Oblak

et al.

Food Chemistry, Journal Year: 2021, Volume and Issue: 373, P. 131594 - 131594

Published: Nov. 14, 2021

The abundance of polyphenols in edible plants makes them an important component human nutrition. Considering the ongoing COVID-19 pandemic, a number studies have investigated as bioactive constituents. We applied in-silico molecular docking well dynamics supported by in-vitro assays to determine inhibitory potential various plant against SARS-CoV-2 therapeutic target, protease 3CLpro. Of initial screening, quercetin, ellagic acid, curcumin, epigallocatechin gallate and resveratrol showed IC50 values 11.8 µM 23.4 µM. In-silico simulations indicated stable interactions with 3CLpro active site over 100 ns production runs. Moreover, surface plasmon resonance spectroscopy was used measure binding real time. Therefore, we provide evidence for inhibition natural polyphenols, suggest further research into development these novel inhibitors or biochemical probes.

Language: Английский

Citations

101
Natural Products, Alone or in Combination with FDA-Approved Drugs, to Treat COVID-19 and Lung Cancer DOI Creative Commons
Liyan Yang, Zhonglei Wang

Biomedicines, Journal Year: 2021, Volume and Issue: 9(6), P. 689 - 689

Published: June 18, 2021

As a public health emergency of international concern, the highly contagious coronavirus disease 2019 (COVID-19) pandemic has been identified as severe threat to lives billions individuals. Lung cancer, malignant tumor with highest mortality rate, brought significant challenges both human and economic development. Natural products may play pivotal role in treating lung diseases. We reviewed published studies relating natural products, used alone or combination US Food Drug Administration-approved drugs, active against acute respiratory syndrome 2 (SARS-CoV-2) cancer from 1 January 2020 31 May 2021. A wide range can be considered promising anti-COVID-19 anti-lung agents have gained widespread attention, including monotherapy for treatment SARS-CoV-2 (ginkgolic acid, shiraiachrome A, resveratrol, baicalein) (daurisoline, graveospene deguelin, erianin) FDA-approved anti-SARS-CoV-2 (cepharanthine plus nelfinavir, linoleic acid remdesivir) (curcumin cisplatin, celastrol gefitinib). demonstrated potential value assistance nanotechnology, drug therapies, codrug strategy, this “natural remedy” could serve starting point further development these

Language: Английский

Citations

90
In-silico evaluation of bioactive compounds from tea as potential SARS-CoV-2 nonstructural protein 16 inhibitors DOI Creative Commons
Rahul Singh, Vijay Kumar Bhardwaj, Jatin Sharma

et al.

Journal of Traditional and Complementary Medicine, Journal Year: 2021, Volume and Issue: 12(1), P. 35 - 43

Published: June 3, 2021

A novel coronavirus, called the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been found to cause COVID-19 in humans and some other mammals. The nonstructural protein 16 (NSP16) of SARS-CoV-2 plays a significant part replication viruses suppresses ability innate immune system detect virus. Therefore, inhibiting NSP16 can be secure path towards identifying potent medication against SARS-CoV-2. Tea (

Language: Английский

Citations

84
Promising Antiviral Activities of Natural Flavonoids against SARS-CoV-2 Targets: Systematic Review DOI Open Access
Ridhima Kaul, Pradipta Paul, Sanjay Kumar

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(20), P. 11069 - 11069

Published: Oct. 14, 2021

The ongoing COVID-19 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) became a globally leading public health concern over past two years. Despite development and administration of multiple vaccines, mutation newer strains challenges to universal immunity has shifted focus lack efficacious drugs for therapeutic intervention disease. As with SARS-CoV, MERS-CoV, other non-respiratory viruses, flavonoids present themselves as promising given their success in silico, vitro, vivo, more recently, clinical studies. This review focuses on data from vitro studies analyzing effects various key SARS-CoV-2 targets presents an analysis structure-activity relationships same. From 27 primary papers, 69 were investigated activities against targets, ranging 3C-like protease (3CLpro) less explored nucleocapsid (N) protein; most quercetin myricetin derivatives, baicalein, baicalin, EGCG, tannic acid. We further silico featuring list involving potential flavonoid-rich extracts combination synthetic or polyphenols suggest prospects future SARS-CoV-2.

Language: Английский

Citations

84
Green Tea Polyphenol (-)-Epigallocatechin-3-Gallate (EGCG): A Time for a New Player in the Treatment of Respiratory Diseases? DOI Creative Commons
Daniela Mokrá, Jana Adamčáková, Juraj Mokrý

et al.

Antioxidants, Journal Year: 2022, Volume and Issue: 11(8), P. 1566 - 1566

Published: Aug. 13, 2022

(-)-Epigallocatechin-3-gallate (EGCG) is a major polyphenol of green tea that possesses wide variety actions. EGCG acts as strong antioxidant which effectively scavenges reactive oxygen species (ROS), inhibits pro-oxidant enzymes including NADPH oxidase, activates systems superoxide dismutase, catalase, or glutathione, and reduces abundant production nitric oxide metabolites by inducible synthase. ECGC also exerts potent anti-inflammatory, anti-fibrotic, pro-apoptotic, anti-tumorous, metabolic effects via modulation intracellular signaling cascades. Based on this knowledge, the use could be benefit in respiratory diseases with acute chronic inflammatory, oxidative, fibrotizing processes their pathogenesis. This article reviews current information biological those animal models has been administered, i.e., distress syndrome, infections, COVID-19, bronchial asthma, obstructive pulmonary disease, lung fibrosis, silicosis, cancer, hypertension, embolism, critically discusses effectiveness administration these disorders. For review, articles English language from PubMed database were used.

Language: Английский

Citations

65
Surface Plasmon Resonance (SPR) Combined Technology: A Powerful Tool for Investigating Interface Phenomena DOI Creative Commons
Yuan Chen, Jialin Xin, Shwu Jen Chang

et al.

Advanced Materials Interfaces, Journal Year: 2023, Volume and Issue: 10(8)

Published: Jan. 29, 2023

Abstract Interface phenomena refer to the caused by various physical and chemical processes occurring at phase interfaces. These can include adhesion, friction, lubrication, evaporation, condensation, adsorption, monolayer formation, other are applied in many fields, such as food, papermaking, rubber, material science, energy, biomedicine. However, traditional detection equipment cannot meet interface observation needs because information detected techniques electrochemistry, spectroscopy, chromatography, mass spectrometry is limited, sensitivity minimum limit. Surface plasmon resonance (SPR) combined technology a powerful tool for investigating phenomena. This paper reviews all combination technologies of SPR with conventional systems, emphasizing Raman spectrometry. observe through provide redox, molecular structure, functional group change, information. Through this information, reactions on be made clearer more controllable, which plays an important role practical detection. In future, will mainly develop toward signal enhancement, light source coupling mode, sensor chip design, achieve real‐time situ

Language: Английский

Citations

34
The Therapeutic Potential of Natural Dietary Flavonoids against SARS-CoV-2 Infection DOI Open Access
Zhonglei Wang, Liyan Yang

Nutrients, Journal Year: 2023, Volume and Issue: 15(15), P. 3443 - 3443

Published: Aug. 3, 2023

The exploration of non-toxic and cost-effective dietary components, such as epigallocatechin 3-gallate myricetin, for health improvement disease treatment has recently attracted substantial research attention. recent COVID-19 pandemic provided a unique opportunity the investigation identification components capable treating viral infections, well gathering evidence needed to address major challenges presented by public emergencies. Dietary hold great potential starting point further drug development prevention SARS-CoV-2 infection owing their good safety, broad-spectrum antiviral activities, multi-organ protective capacity. Here, we review current knowledge characteristics-chemical composition, bioactive properties, putative mechanisms action-of natural flavonoids with targeting its variants. Notably, present promising strategies (combination therapy, lead optimization, delivery) overcome inherent deficiencies flavonoids, limited bioavailability poor stability.

Language: Английский

Citations

25