International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 275, P. 133229 - 133229
Published: June 17, 2024
Language: Английский
Carbohydrate Polymers, Journal Year: 2023, Volume and Issue: 321, P. 121319 - 121319
Published: Aug. 22, 2023
Language: Английский
Citations
52
International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 126709 - 126709
Published: Sept. 9, 2023
Language: Английский
Citations
37
Journal of Composites Science, Journal Year: 2023, Volume and Issue: 7(12), P. 488 - 488
Published: Nov. 26, 2023
For over a century, enzyme immobilisation has been proven to be superior strategy improve catalytic activity and reusability ensure easy separation, operation, reduced cost. Enzyme allows for an easier separation of the from reaction mixture, thus simplifying downstream processing. This technology protects degradation or inactivation by harsh conditions, making it more robust suitable used in various applications. Recent strategies methods, such as adsorption, cross-linking, entrapment encapsulation, covalent bonding, were critically reviewed. These have shown promising results improving stability, activity, A recent development nanomaterials agrowaste renewable carriers is underlined current review. Furthermore, use gained significant attention due their unique properties, high surface area, mass transfer, biocompatibility, sustainability. materials offer outcomes developing efficient sustainable immobilised enzymes. state-of-the-art better control reactions enhances reusability, leading cost-effective environmentally friendly processes. The also helps reduce waste generation promote utilisation resources, further contributing circular economy.
Language: Английский
Citations
37
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130730 - 130730
Published: March 11, 2024
Language: Английский
Citations
12
BMEMat, Journal Year: 2024, Volume and Issue: unknown
Published: March 12, 2024
Abstract In order to co‐immobilize multiple enzymes, a wide range of nanomaterials has been designed achieve synergistic enzyme activity and enhance catalytic efficiency. Nanomaterials, as carriers for co‐immobilization, possess various advantages such tunable morphology size, high specific surface area, abundant chemically active sites. They can significantly stability, activity, We overview the commonly used methods strategies co‐immobilization. This review further summarizes latest research advances in co‐immobilization applications over past 5 years. Meanwhile, challenges these well some potential future directions are also discussed.
Language: Английский
Citations
11
Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(22)
Published: April 1, 2024
Abstract The design and orderly layered co‐immobilization of multiple enzymes on resin particles remain challenging. In this study, the SpyTag/SpyCatcher binding pair was fused to N‐terminus an alcohol dehydrogenase (ADH) aldo‐keto reductase (AKR), respectively. A non‐canonical amino acid (ncAA), p ‐azido‐L‐phenylalanine (p‐AzF), as anchor for covalent bonding enzymes, genetically inserted into preselected sites in AKR ADH. Employing two bioorthogonal counterparts azide–alkyne cycloaddition immobilization ADH enabled sequential dual‐enzyme coating porous microspheres. ordered reactor subsequently used synthesize ( S )‐1‐(2‐chlorophenyl)ethanol asymmetrically from corresponding prochiral ketone, enabling situ regeneration NADPH. exhibited a high catalytic conversion 74 % good reproducibility, retaining 80 its initial activity after six cycles. product had 99.9 ee, which that maintained each cycle. Additionally, double‐layer method significantly increased enzyme loading capacity, approximately 1.7 times greater than traditional single‐layer immobilization. More importantly, it simultaneously both purification carriers, thus providing convenient approach facilitate cascade biocatalysis.
Language: Английский
Citations
9
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 260, P. 129362 - 129362
Published: Jan. 23, 2024
Language: Английский
Citations
8
ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(17), P. 6564 - 6572
Published: April 18, 2024
In the present article, glutaraldehyde was used to covalently coimmobilize lipase Eversa Transform 2.0 and β-galactosidase from Aspergillus oryzae. Both enzymes were adsorbed on amino supports modified with glutaraldehyde. However, first enzyme remained almost fully active under stress conditions, while lost a large percentage of its activity. To prevent necessity discarding both enzymes, immobilized following this immobilization strategy. The biocatalyst reduced eliminate chemical reactivity, then coimmobilized via ion exchange. incubation at high concentrations salt desorbed support. This combi-biocatalyst in three inactivation/rebuilding cycles where inactivated liberated by washing ionic strength replaced fresh enzyme, maintained activity throughout 3 cycles. That way, it possible use strategy reuse build new combi-biocatalysts after inactivation.
Language: Английский
Citations
6
Carbohydrate Polymers, Journal Year: 2023, Volume and Issue: 327, P. 121691 - 121691
Published: Dec. 16, 2023
Language: Английский
Citations
14
Green Chemistry, Journal Year: 2024, Volume and Issue: 26(10), P. 6180 - 6189
Published: Jan. 1, 2024
One-pot cascade biocatalysis for the conversion of renewable lignin-derived phenol and catechol into hydroxytyrosol.
Language: Английский
Citations
4