Smart chemistry and applied perceptions of enzyme-coupled nano-engineered assemblies to meet future biocatalytic challenges

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 493, P. 215329 - 215329

Published: July 9, 2023

Language: Английский

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Organic–inorganic hybrid nanoflowers: A comprehensive review of current trends, advances, and future perspectives

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 489, P. 215191 - 215191

Published: May 6, 2023

Language: Английский

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Role of pretty nanoflowers as novel versatile analytical tools for sensing in biomedical and bioanalytical applications

Smart Medicine, Journal Year: 2024, Volume and Issue: 3(1)

Published: Feb. 1, 2024

In recent years, an encouraging breakthrough in the synthesis of immobilized enzymes flower-shaped called "organic-inorganic hybrid nanoflowers (hNFs)" with greatly enhanced catalytic activity and stability were reported. Although, these hNFs discovered by accident, exhibited highly activities stabilities compared free conventionally enzymes. Herein, we rationally utilized for analytical applications. this comprehensive review, covered design use as novel versatile sensors electrochemical, colorimetric/optical immunosensors-based detection strategies perspective.

Language: Английский

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Immobilization of collagenase in inorganic hybrid nanoflowers with enhanced stability, proteolytic activity, and their anti-amyloid potential

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 274, P. 133114 - 133114

Published: June 12, 2024

Language: Английский

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Hyperactivation of lipases by immobilization on superhydrophobic graphene quantum dots inorganic hybrid nanoflower

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 254, P. 127817 - 127817

Published: Nov. 1, 2023

Language: Английский

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Synthesis of chiral alcohol (S)-CHBE by co-immobilization of double enzymes based on organic-inorganic hybrid nanoflower

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 139645 - 139645

Published: Jan. 1, 2025

Language: Английский

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The Effects of Sika Deer Antler Peptides on 3T3-L1 Preadipocytes and C57BL/6 Mice via Activating AMPK Signaling and Gut Microbiota

Molecules, Journal Year: 2025, Volume and Issue: 30(5), P. 1173 - 1173

Published: March 6, 2025

(1) Background: To explore the anti-obesity effects and mechanisms of sika deer velvet antler peptides (sVAP) on 3T3-L1 preadipocytes in high-fat diet (HFD)-induced obese mice. (2) Methods: sVAP fractions different molecular weights were obtained via enzymatic hydrolysis ultrafiltration. Their anti-lipid cells assessed with Oil Red O staining. The optimal fraction was tested HFD-induced C57BL/6 mice to mechanisms. Peptide purification used LC-MS/MS, followed by sequence analysis docking for activity prediction. (3) Results: peptide best identified as sVAP-3K (≤3 kDa). reduced lipid content proliferation cells, improved profiles ameliorated adipocyte degeneration HFD mice, promoted growth beneficial gut microbiota, maintained metabolism. Additionally, activated AMP-activated protein kinase (AMPK) signaling pathway, regulating adipogenic transcription factors. exhibited ten major components (peak area ≥ 1.03 × 108), four most active being newly discovered natural oligopeptides: RVDPVNFKL (m/z 363.21371), GGEFTPVLQ 474.24643), VDPENFRL 495.25735), VDPVNFK 818.44043). (4) Conclusion: This study identifies novel oligopeptides key effects, offering new evidence developing weight-loss drugs from velvet.

Language: Английский

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Dipeptide–Copper Hybrid Nanoparticles as Efficient Laccase Mimics for Determination and Degradation of Phenolic Pollutants and Epinephrine

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: April 13, 2025

The broad application of laccase mimics is notably constrained by their costly and intricate synthesis routes. For example, researchers often use peptides to coordinate with copper ions mimic the active sites laccases, thereby constructing mimics. However, these synthetic methods are quite complex, such as requiring high temperatures, organic solvents, long durations, cumbersome procedures. In this study, we introduce a straightforward yet highly active, robust, versatile known HH-Cu, which was synthesized through simple precipitation reaction combining dipeptide HH Cu2+ in phosphate-buffered saline (PBS). By adjusting ratio ions, were able produce most effective organic-inorganic hybrid nanoparticles, namely, outperforms HH-Cu nanoflowers. demonstrates extraordinary catalytic efficiency, 4.7-fold higher maximum velocity (v max) lower Michaelis constant (K m) compared natural laccase. This nanozyme also remarkably resilient under harsh conditions extreme pH levels, salinity, it shows excellent storage stability reusability. We successfully applied for degradation quantitative detection various phenolic pollutants epinephrine. markedly superior sensitivity detecting epinephrine than offers comparable limit broader linear range other research should lay groundwork ongoing efforts development nanozymes provide an method efficient

Language: Английский

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Transforming insects into a conventional food source using biotechnological processes

Biocatalysis and Agricultural Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 103598 - 103598

Published: May 1, 2025

Language: Английский

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Nanoflower Microreactor Based Versatile Enhancer for Recognition Cofactor-Dependent Enzyme Biocatalysis toward Saxitoxin Detection

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(35), P. 46495 - 46505

Published: Aug. 21, 2024

Investigating organic carriers' utilization efficiency and bioactivity within organic–inorganic hybrid nanoflowers is critical to constructing sensitive immunosensors. Nevertheless, the sensitivity of immunosensors interactively regulated by different classes biomolecules such as antibodies enzymes. In this work, we introduced a new alkaline phosphatase–antibody–CaHPO4 (AAHNFs) microreactor based colorimetric immunoprobe. This system integrates biometric unit (antibody) with signal amplification element (enzyme) through biomineralization process. Specifically, factors affecting antibody recognition activity in formation mechanism AAHNFs are investigated. The designed retain ability enhanced protection for encapsulated proteins against high temperature, solvents, long-term storage, facilitating selective construction lock structures antigens. Additionally, immunosensor on was developed. After ascorbic acid 2-phosphate hydrolysis phosphatase (ALP), generated decomposes I2 I–, inducing localized surface plasmon resonance silver nanoplate, which effectively tuned shape conversion develop sensor. Further, 3D-printed portable device fabricated, integrated smartphone sensing platform, applied data collection analysis. Notably, exhibits improved analytical performance 0.1–6.25 ng·mL–1 detection range 0.06 limit quantitative saxitoxin (STX) average recoveries STX real samples ranged from 85.9% 105.9%. study presents more in-depth investigation performance, providing insights practical applications.

Language: Английский

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