Frontiers in Marine Science,
Journal Year:
2024,
Volume and Issue:
11
Published: Nov. 4, 2024
Global
climate
change
has
caused
rapid
temperature
changes
in
marine
environments.
Understanding
how
organisms
respond
to
can
help
predict
their
richness
of
future
biodiversity.
In
this
study,
we
examined
the
gene
expression
levels
and
difference
pathways
that
are
responsive
acute
stress
low-
high-latitude
populations
shore
swimming
crab,
Charybdis
japonica
.
The
two
C.
were
exposed
high-temperature
stresses
(15°C
28°C)
used
for
transcriptome
sequencing.
Genetic
regulatory
ability
compared
determine
diverse
response
crab
change.
functional
enrichment
analysis
showed
low-latitude
regulated
more
genes
(938)
mainly
enriched
DNA
replication
metabolic
pathways,
whereas
less
(309)
genetic
information
processing
at
low-temperature
stress.
Furthermore,
(33)
processing,
(280)
signal
transduction
cellular
process
These
results
implied
population
was
resilient
stress,
while
This
study
enhances
our
understanding
different
geographic
varying
environments
living
zone,
which
could
be
helpful
predicting
biodiversity
trends
intertidal
crustaceans
under
global
Insect Molecular Biology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 27, 2025
The
cuticle
of
insects
serves
as
a
crucial
organ
for
preserving
body
composition,
protecting
against
pathogen
invasion,
and
retaining
moisture
in
their
bodies.
Cuticular
proteins
(CPs)
are
the
main
constituents
insect
cuticles
interact
with
chitin
to
form
cuticle's
structural
framework
mechanical
properties.
In
this
study,
we
investigated
role
cuticular
protein
R&R
consensus
(CPR),
BmorCPR67,
member
RR-2
subfamily,
during
prepupal-to-pupal
transition
Bombyx
mori.
BmorCPR67
gene
exhibited
high
expression
levels
prepupal
stage,
highest
detected
epidermis
day-1
pupa
B.
was
induced
by
20-hydroxyecdysone
(20E).
Chitin-binding
assays
indicated
that
selectively
binds
crystalline
chitosan
but
not
amorphous
chitin.
Silencing
disrupted
moulting
process
from
prepupa
pupa,
resulting
silkworm
mortality.
Furthermore,
knockdown
altered
profiles
key
genes
involved
metabolism.
Notably,
significant
thinning
endocuticle
observed
48-96
h
after
siRNA
injection
BmorCPR67-silenced
silkworms.
These
findings
highlight
critical
development
mori,
contributing
our
understanding
functions
CPs
metamorphosis.
Genes,
Journal Year:
2023,
Volume and Issue:
14(8), P. 1556 - 1556
Published: July 29, 2023
Salt
stress
is
an
important
environmental
factor
affecting
crop
growth
and
development.
One
of
the
ways
to
improve
salt
tolerance
rice
identify
new
salt-tolerance
genes,
reveal
possible
mechanisms,
apply
them
creation
germplasm
breeding
varieties.
In
this
study,
salt-sensitive
japonica
variety
Tong
35
(T35)
salt-tolerant
Ji
Nongda
709
(JND709)
were
used.
treatment
with
a
150
mmol/L
NaCl
solution
(the
control
group
was
tested
without
simultaneously)
continued
until
test
material
collected
after
germination
period.
Twelve
cDNA
libraries
constructed,
5
comparator
groups
established
for
transcriptome
sequencing.
On
average,
9.57G
raw
sequencing
data
generated
per
sample,
alignment
reference
genome
above
96.88%
guanine-cytosine
(GC)
content
53.86%.
A
total
16,829
differentially
expressed
genes
present
in
five
comparison
groups,
which
2390
specifically
T35
(category
1),
3306
JND709
2),
1708
both
breeds
3).
Differentially
subjected
gene
ontology
(GO),
functional
enrichment
analysis,
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
pathway
revealed
that
these
belonged
three
main
classes:
molecular
function,
cellular
components,
biological
processes.
KEGG
analysis
showed
significantly
enriched
pathways
included
phenylpropane
biosynthesis,
phytohormone
signaling,
interaction
plants
pathogens.
we
provided
studying
mechanism
underlying
during
germination.
Archives of Insect Biochemistry and Physiology,
Journal Year:
2024,
Volume and Issue:
115(3)
Published: March 1, 2024
Abstract
The
structural
cuticle
proteins
(CPs)
play
important
roles
in
the
development
and
fitness
of
insects.
However,
knowledge
about
CP
gene
superfamily
is
limited
virus‐transmitting
insect
vectors,
although
its
importance
on
transmission
plant
virus
has
been
gradually
emphasized.
In
this
study,
genome‐wide
identification
was
conducted
western
flower
thrips
Frankliniella
occidentalis
that
globally
invasive
pest
vector
pest.
transmits
notorious
tomato
spotted
wilt
(TSWV)
around
world,
causing
large
damage
to
a
wide
array
plants.
One
hundred
twenty‐eight
F.
genes
(
FoCP
s)
were
annotated
study
they
classified
into
10
distinct
families,
including
68
CPR
s,
16
CPAP1
6
CPAP3
2
CPCFC
Tweedle
4
CPF
CPLCP
CPG
s.
comprehensive
analysis
performed
phylogenetic
relationship,
location
expression
profiles
during
different
stages
.
Transcriptome
revealed
more
than
30%
s
upregulated
at
least
1.5‐fold
when
infected
by
TSWV,
indicating
their
potential
involvement
TSWV
interactions.
Our
provided
an
overview
superfamily.
gave
better
understand
CP's
role
transmission,
which
clues
for
reducing
viral
damages
through
silencing
vectors.
