Transcriptional Control of Seed Life: New Insights into the Role of the NAC Family

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5369 - 5369

Published: May 14, 2024

Transcription factors (TFs) regulate gene expression by binding to specific sequences on DNA through their DNA-binding domain (DBD), a universal process. This update conveys information about the diverse roles of TFs, focusing NACs (NAM-ATAF-CUC), in regulating target-gene and influencing various aspects plant biology. NAC TFs appeared before emergence land plants. The family constitutes group plant-specific found mosses, conifers, monocots, eudicots. discusses evolutionary origins genes/proteins from green algae crucial development stress response across species. From mosses lycophytes angiosperms, number proteins increases significantly, suggesting gradual evolution basal streptophytic algae. play critical role enhancing abiotic tolerance, with function conserved angiosperms. Furthermore, modular organization NACs, dimeric function, localization within cellular compartments contribute functional versatility complexity. While most are nuclear-localized active, subset is other compartments, indicating inactive forms until cues trigger translocation nucleus. Additionally, it highlights involvement endoplasmic reticulum (ER) stress-induced programmed cell death (PCD) activating vacuolar processing enzyme (VPE) gene. Moreover, this provides comprehensive overview plants, including participation ER responses, leaf senescence (LS), growth development. Notably, exhibit correlations phytohormones (i.e., ABA, GAs, CK, IAA, JA, SA), several genes inducible them, broad spectrum biological processes. study spatiotemporal patterns insights into when where shedding light metabolic contributions. Likewise, review emphasizes significance transcriptional modules, seed reserve accumulation, regulation dormancy germination. Overall, effectively communicates intricate essential functions Finally, an standpoint, phylogenetic analysis suggests that highly probable WRKY evolutionarily older than family.

Language: Английский

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Application of Salicylic Acid Improves the Production of Medicinal Components in Mucuna macrocarpa Wall by Regulating Endogenous Hormone and Nutrient Balance

Plants, Journal Year: 2025, Volume and Issue: 14(7), P. 1023 - 1023

Published: March 25, 2025

The Mucuna macrocarpa Wall, a traditional Chinese medicinal plant, exhibits significant cultivation-dependent variations in the accumulation and yield of its components. Salicylic acid (SA) has demonstrated potential to regulate plant growth, which can be strategically used enhance yield, offering promising approach for high-yield cultivation plants. This study aimed investigate changes components seedlings (4 months old) at different concentrations SA (0, 0.1, 0.5, 0.9, 1.3 mM) pot experiment. results indicate that significantly increased basal diameter (BD) by 2.9% 20.61% total biomass (TB) 14.28% 48.57%. Notably, treatments resulted alterations endogenous hormone content, including indole-3-acetic (IAA), abscisic (ABA), gibberellin A3 (GA3), trans-zeatin-riboside (ZR), balance leaves. regulated content nitrogen (N), phosphorus (P), potassium (K) all organs, K K: P roots, stems, leaves was higher than control under 0.9 mM treatment. Crucially, enhanced bioactive compounds. phenolic (TPC) flavonoid (TFC) stems peaked (14.89 mg·g-1 3.73 mg·g-1, respectively), were 11.87% 11.68% those control. Moreover, compared control, production 20.00% 61.45% 3.89% 90.56%. In addition, found more effective other increasing d content. summary, this investigated effect as an eco-friendly elicitor improve macrocarpa.

Language: Английский

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Brassinosteroid-related transcription factor BZR1 regulates vegetative development and flavonoids biosynthesis in Scutellaria baicalensis

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142383 - 142383

Published: March 1, 2025

Language: Английский

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Characterization of the NAC gene family in ‘Fengdan’ peony (Paeonia ostii) insights into the evolution and expression patterns under abiotic stresses and ABA treatment

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 16, 2025

As one of the largest plant-specific transcription factor families, NAC proteins are crucial for plant growth and development processes responses to various abiotic biotic stresses. The published sequenced chromosome-level genome 'Fengdan'peony provides a powerful tool analysis gene family in this shrub. PoNAC was identified characterized using bioinformatic analysis, RT-qPCR performed on some PoNACs from ATAF NAP subfamilies. In study, total 82 factors (TFs) were 'Fengdan' peony genome, with uneven anchorage 78 genes 5 chromosomes, whereas only 4 found be located unanchored scaffolds. Through phylogenetic 66 classified into 15 distinct structure revealed variation number exons 0 14. Moreover, motif indicated that TFs possessed conserved domains motifs. duplication events included whole-genome duplications (WGDs) or segmental 14 pairs, tandem 2 proximal 3 pairs. GO results suggested functions mostly concentrated "biological process" category. Additionally, expression profiles different organs 45 expressed tissues, them exhibited tissue-specific related development. experiments demonstrated 8 subfamilies ABA, heat drought, suggesting they may serve as important regulatory factor.

Language: Английский

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Comprehensive analysis of the NAC transcription factor gene family in Sophora tonkinensis Gagnep

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 25, 2025

Sophora tonkinensis Gagnep. has long been utilized in the treatment of anti-inflammatory and pain-relieving, with its principal active compounds being alkaloids flavonoids. NAC transcription factors, a large family plant-specific regulators, play pivotal roles growth, development, stress responses, secondary metabolism. However, comprehensive genome-wide characterization S. gene (StNAC) remains unexplored. This study identified 85 proteins from genome database. Phylogenetic analysis revealed that StNAC were categorized into 15 subgroups based on their homology Arabidopsis thaliana proteins. Gene structure demonstrated variation intron numbers ranging 1 to 7, majority genes containing 2-3 introns. Chromosomal distribution indicated an uneven spread across 9 chromosomes, highest number Chr3. Detection 4 tandem duplicates 32 segmental duplication primarily drive amplification. Prediction cis-regulatory elements suggested involvement hormone regulation. expression showed substantial variability different tissues. Notably, eight as significantly associated alkaloid flavonoid levels. qRT-PCR validation five highly expressed tissues, corroborating transcriptome data. These findings offer valuable insights for further functional potential biosynthesis tonkinensis.

Language: Английский

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Comprehensive Analysis of NAC Transcription Factors Reveals Their Evolution in Malvales and Functional Characterization of AsNAC019 and AsNAC098 in Aquilaria sinensis

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(24), P. 17384 - 17384

Published: Dec. 12, 2023

NAC is a class of plant-specific transcription factors that are widely involved in the growth, development and (a)biotic stress response plants. However, their molecular evolution has not been extensively studied Malvales, especially Aquilaria sinensis, commercial horticultural crop produces an aromatic resin named agarwood. In this study, 1502 members gene family were identified from genomes nine species Malvales three model The macroevolutionary analysis revealed whole genome duplication (WGD) dispersed (DSD) have shaped current architectural structure families Then, 111 genes systemically characterized A. sinensis. phylogenetic suggests sinensis can be classified into 16 known clusters four new subfamilies, with each subfamily presenting similar structures conserved motifs. RNA-seq showed AsNACs presents broad transcriptional to agarwood inducer. expression patterns 15 after injury treatment indicated AsNAC019 AsNAC098 positively correlated polyketide synthase (PKS) genes. Additionally, also found bind AsPKS07 promoter activate its transcription. This comprehensive provides valuable insights plants highlights potential mechanisms for regulating secondary metabolite biosynthesis 2-(2-phenyl) chromones

Language: Английский

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Identification of Eleutherococcus senticosus NAC transcription factors and their mechanisms in mediating DNA methylation of EsFPS, EsSS, and EsSE promoters to regulate saponin synthesis

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: May 31, 2024

Abstract Background The formation of pharmacologically active components in medicinal plants is significantly impacted by DNA methylation. However, the exact mechanisms through which methylation regulates secondary metabolism remain incompletely understood. Research model species has demonstrated that at transcription factor binding site within functional gene promoters can impact factors to target DNA, subsequently influencing expression. These findings suggest interaction between and could be a significant mechanism plants. Results This research conducted comprehensive analysis NAC family E. senticosus , encompassing genome-wide characterization analysis. A total 117 EsNAC genes were identified phylogenetically divided into 15 subfamilies. Tandem duplications chromosome segment found primary replication modes these genes. Motif 2 was as core conserved motif genes, cis-acting elements, structures, expression patterns each different. Es JUB1, NAC047, NAC098, NAC005 associated with ratio senticosus. four located nucleus or cytoplasm exhibited transcriptional self-activation activity. EsFPS EsSS EsSE reduced their methyl groups added cytosine directly hindered altered eventually leading changes saponin synthesis Conclusions are from methylated . incapacity NACs bind promoter synthase leads subsequent alterations synthesis. initial evidence showcasing involvement governing on production

Language: Английский

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Abscisic acid induces PpeKIL1 to terminate fruit growth and promote fruit abortion in peach (Prunus persica)

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 212, P. 108761 - 108761

Published: May 21, 2024

Language: Английский

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Effects of elicitors from culture filtrate of Fusarium solani CL105 on flavonoid production of Scutellaria baicalensis calli

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: June 4, 2024

Introduction Endophytic fungi can promote secondary metabolite accumulation in medicinal plants. Previously, we observed that the culture filtrate of Fusarium solani CL105 promoted flavonoid production Scutellaria baicalensis calli. However, active ingredients and mechanisms associated with this remain unclear. Methods This study evaluates effects different elicitors from F. namely, exopolysaccharide (EPS), exoprotein (EP), other parts (OP), on S. calli by HPLC. Subsequently, underlying mechanism EPS induced was revealed transcriptomics RT-PCR. Results discussion The results indicated a significant increase following treatment EPS. Baicalin (1.40 fold), wogonoside (1.91 wogonin (2.76 fold) were most significantly up-regulated compared control. Transcriptome analysis further up-regulation key enzyme genes ( CHS, CHI, FNS , F6H ) involved synthesis after 5 days treatment. Moreover, expression GA2ox CYP707A —genes gibberellin acid (GA) abscisic biosynthesis (ABA), respectively—were up-regulated. levels certain transcription factors, including MYB3, MYB8 MYB13 also higher than controls. Our main elicitor promoting We postulated might stimulate MYB8, MYB13, leading to markedly upregulated levels, ultimately synthesis. provides novel avenue for large-scale vitro flavonoids .

Language: Английский

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Sulfur redirects carbon metabolism to optimize nitrogen utilization and promote andrographolide biosynthesis in Andrographis paniculata seedlings

Current Plant Biology, Journal Year: 2024, Volume and Issue: unknown, P. 100422 - 100422

Published: Nov. 1, 2024

Language: Английский

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