Isolation and Experimental Study on Bacteriostasis of Bacillus haynesii DOI Open Access

Jiedan Liao,

Mengbo Yu,

Jiaming Li

et al.

Journal of Clinical and Nursing Research, Journal Year: 2024, Volume and Issue: 8(7), P. 219 - 227

Published: Aug. 13, 2024

This experiment aims to isolate and inhibit three bacteria strains provide candidate for the development application of probiotics. Using bacterial morphological identification, 16S rDNA sequence alignment, genetic evolution analysis, were identified as Bacillus haynesii, named HP01, HD02, HK03. Through biosurfactant activity tests, C-TAB hemolysis antibacterial analyses, results showed that all B. haynesii exhibited significant activity. Additionally, solutions demonstrated a pronounced effect on Staphylococcus aureus. The resistance safety commonly used drugs evaluated using tablet diffusion method mouse feeding test. indicated not resistant drugs, oral solution was pathogenic had high in mice. study concluded met basic conditions use, with HP01 being most promising candidate.

Language: Английский

Valorization of pineapple (ANANAS comosus) peel waste for levan production: Assessment of biological activities DOI

Kumaresan Priyanka,

Jilnasree Naduvile Purayil,

K. Preethi

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 139482 - 139482

Published: Jan. 1, 2025

Language: Английский

Citations

1
Vat-Based 3D-Bioprinted Scaffolds from Photocurable Bacterial Levan for Osteogenesis and Immunomodulation DOI
Yusuf Olatunji Waidi,

Vasudev S. Wagh,

Sumeet Mishra

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 11, 2025

Emerging techniques of additive manufacturing, such as vat-based three-dimensional (3D) bioprinting, offer novel routes to prepare personalized scaffolds complex geometries. However, there is a need develop bioinks suitable for clinical translation. This study explored the potential bacterial-sourced methacrylate levan (LeMA) bioink digital light processing (DLP) 3D bioprinting bone tissue scaffolds. LeMA was successfully synthesized, characterized, and used fabricate 3D-bioprinted with excellent printability physicochemical properties. In vitro studies demonstrated superior cytocompatibility 15% w/v gels compared 20% gels. supported osteogenic differentiation , evidenced by alkaline phosphatase activity mineral deposition MC3T3 pre-osteoblasts. Importantly, hydrogels positively modulated macrophage phenotype, promoting expression anti-inflammatory marker CD206. These findings suggest that 3D-printed can create favorable microenvironment regeneration, highlighting their repair regeneration applications.

Language: Английский

Citations

1
Production and Characterization of Extracellular Polysaccharide from Anoxybacillus flavithermus AAG-61 with Resistance to Sulfamethoxazole and Ofloxacin DOI Creative Commons
Berna Genç

Nepal Journal of Biotechnology, Journal Year: 2024, Volume and Issue: 12(1)

Published: July 31, 2024

Extracellular polysaccharide producers from Kursunlu (Manisa) thermal spring were identified and a total of nine isolates selected. One isolate was selected based on its strong mucoid structure as Anoxybacillus flavithermus (AAG61) by MALDI-TOF MS. A. AAG-61 had multiple antibiotic resistance to both Sulfamethoxazole, Ofloxacin. The produced purified characterized. It an anionic heteropolysaccharide containing rhamnose, mannose, arabinose, glucose. water-soluble concentration 3.1 g/L carbohydrate 0.11 protein. EPS consisted 32% carbon, 7% nitrogen, 5% hydrogen, traces sulfur (0.6%). presence protein in the confirmed FT-IR. Zeta potential, mobility, conductivity values -8.3 (± 0.5) mV, -0.65 0.04) µmcm/Vs, 1.57 0.08) mS/cm. crystallinity pattern also determined XRD analysis. No effect viability human fibroblast cell line observed.

Language: Английский

Citations

0
Isolation and Experimental Study on Bacteriostasis of Bacillus haynesii DOI Open Access

Jiedan Liao,

Mengbo Yu,

Jiaming Li

et al.

Journal of Clinical and Nursing Research, Journal Year: 2024, Volume and Issue: 8(7), P. 219 - 227

Published: Aug. 13, 2024

This experiment aims to isolate and inhibit three bacteria strains provide candidate for the development application of probiotics. Using bacterial morphological identification, 16S rDNA sequence alignment, genetic evolution analysis, were identified as Bacillus haynesii, named HP01, HD02, HK03. Through biosurfactant activity tests, C-TAB hemolysis antibacterial analyses, results showed that all B. haynesii exhibited significant activity. Additionally, solutions demonstrated a pronounced effect on Staphylococcus aureus. The resistance safety commonly used drugs evaluated using tablet diffusion method mouse feeding test. indicated not resistant drugs, oral solution was pathogenic had high in mice. study concluded met basic conditions use, with HP01 being most promising candidate.

Language: Английский

Citations

0