Integrative Transcriptomics and Proteomics Analysis Reveals THRSP’s Role in Lipid Metabolism DOI Open Access
Yujie Li, Ke Xu,

Ao Zhou

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(12), P. 1562 - 1562

Published: Nov. 30, 2024

Background/Objectives: Abnormalities in lipid metabolism and endoplasmic reticulum (ER) stress are strongly associated with the development of a multitude pathological conditions, including nonalcoholic fatty liver disease (NAFLD), diabetes mellitus, obesity. Previous studies have indicated potential connection between thyroid hormone responsive (THRSP) that ER may participate synthesis key regulators adipogenesis. However, specific mechanisms remain to be investigated. Methods: In this study, we explored roles THRSP by interfering gene expression mouse mesenchymal stem cells, comparing effects on adipogenesis control interfered groups, combining transcriptomic proteomic analysis. Results: Our results showed number droplets was significantly reduced after THRSP, levels adipogenesis, such as LPL, FABP4, PLIN1, CIDEC, were downregulated. Both differential genes (proteins) enriched processes lipolytic regulation, stress, cholesterol metabolism, sphingolipid PPAR signaling pathway, glycerophospholipid metabolism. The marker gene, ATF6, most downregulated transcription factor. addition, RT-qPCR validation pathway SCD1; droplet generation LIPE, DGAT1, AGPAT2; ATF6 Conclusions: These suggest is involved regulating which closely related Interfering helpful ameliorating occurrence diseases abnormalities

Language: Английский

Consumption of interesterified palm oil leads inflammation of white adipose tissue and triggers metabolic disturbances in mice on a high-fat diet DOI Creative Commons
Bruna Cadete Martins,

Mayara da Silva Ribeiro,

Ananda Vitória Silva Teixeira

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 31, 2024

Abstract Growing obesity is linked to shifts in dietary patterns, particularly the increased intake of ultra-processed high-fat foods. This study aimed evaluate effects interesterified palm oil consumption on glucose homeostasis, adipose tissue remodeling, and hepatic lipogenesis C57BL/6 mice fed a diet. Sixty were divided into four groups (n = 15): control group (C) standard diet (4% soybean oil), (HF) (23.8% lard), high fat (HFP) (HFI) oil) for 8 weeks (all received 50% energy from lipids). The HFI exhibited higher body mass than HF (+ 11%, P < 0.05), which was attributed an percentage mass. Plasma concentrations IL-6, insulin, HOMA-IR also elevated group. Both HFP showed hypertrophied adipocytes pancreatic islets, alpha beta cell masses, steatosis, low expression genes related beta-oxidation, upregulated lipogenesis. In conclusion, alters inflammatory profiles.

Language: Английский

Citations

7
Multi‐miRNAs‐Mediated Hepatic Lepr Axis Suppression: A Pparg–Dicer1 Pathway‐Driven Mechanism in Spermatogenesis for the Intergenerational Transmission of Paternal Metabolic Syndrome DOI Creative Commons
Yi Lin, Xiuye Ni, Lin Zhu

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Abstract Bisphenol A (BPA) is an “environmental obesogen” and this study aims to investigate the intergenerational impacts of BPA‐induced metabolic syndrome (MetS), specifically focusing on unraveling mechanisms. Exposure BPA induces disorders in paternal mice, which are then transmitted offspring, leading late‐onset MetS. Mechanistically, upregulates Srebf1, turn promotes Pparg‐dependent transcription Dicer1 spermatocytes, increasing levels multiple sperm microRNAs (miRNAs). Several these miRNAs highly expressed a synchronized manner liver offspring. miR149‐5p, miR150‐5p, miR700‐5p target specific region Lepr 3′UTR, termed “SMITE” (“ S everal Mi RNAs T argeting E lements”), negatively regulate Lepr. These i nherited nti‐ L epr miRNAs, also referred (IAL‐miRs), modulate hepatic steatosis, insulin signaling through regulatory Igfbp2, Egfr, Ampk. Furthermore, IAL‐miRs inhibit Ccnd1 not only via binding but Lepr–Igfbp2 axis, contribute hepatocyte senescence. pathological processes interact self‐reinforcing cycle, worsening MetS BPA‐exposed The findings reveal mechanism wherein lipid metabolism reprogramming spermatocytes‐induced perturbations triggered by BPA, leads inheritance suppression axis

Language: Английский

Citations

0
Arginase 1 promotes hepatic lipogenesis by regulating ERK2/PPARγ signaling in a non-canonical manner DOI Creative Commons
Yujun Shi,

Mingyang Shao,

Xiaoyue Cao

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Abstract The global incidence of obesity and its metabolic sequelae, notably dysfunction-associated steatohepatitis (MASLD), has escalated to epidemic levels. We unveil a previously unknown moonlighting role for arginase 1 (Arg1) in facilitating hepatic lipogenesis. Mice lacking Arg1 exhibited diminished lipid accumulation both liver adipocytes, an effect mirrored genetically- or diet-induced models following inhibitor treatment. Mechanistically, competes with RSK2 Elk1 binding the substrate-binding pocket extracellular signal-regulated kinase 2 (ERK2) via S-shaped motif, thereby enhancing ERK2 ubiquitination degradation upregulating AKT/mTOR/PPARγ Elk1/c-Fos/PPARγ cascades, ultimately augmenting Peptides designed mimic disrupted Arg1-ERK2 interaction improved profiles MASLD models. Our findings implicate regulates metabolism physical ERK2, highlighting as promising therapeutic target related disorders.

Language: Английский

Citations

0
NOD1: a metabolic modulator DOI Creative Commons

Ruobing Tang,

Chunguang Xie,

X.L. Zhang

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 21, 2025

Nucleotide-binding oligomerization domain 1 (NOD1) is an intracellular pattern recognition receptor that detects injury signals and initiates inflammatory responses host defense. Furthermore, NOD1 serves as a metabolic mediator by influencing the metabolism of various tissues, including adipose tissue, liver, cardiovascular pancreatic β cells, adrenal glands, bones through diverse mechanisms. It has been discovered activated associated with pathological mechanisms certain diseases. This review presents comprehensive summary impact on tissue-specific metabolism.

Language: Английский

Citations

0
Transcriptome Reveals the Promoting Effect of Beta-Sitosterol on the Differentiation of Bovine Preadipocytes DOI
Lei Jiang, Xiaolei Sun, Yuan Wan

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Natural small molecule compounds play crucial roles in regulating fat deposition. Beta-sitosterol exhibits multiple biological activities such as cholesterol reduction and anticancer effects. However, its regulatory mechanism the differentiation of bovine preadipocytes remains unclear. We identified potential associations with processes regulation lipid metabolism through prediction targets. utilized techniques Oil Red O staining, Western blotting, RNA-seq, others to elucidate promoting effect on preadipocytes. Furthermore, reducing expression most downregulated gene among differential expressed genes (DEGs), MGP, promotes After interfering RNA-seq analysis sixth day revealed that DEGs were significantly enriched PPAR signaling pathway. In this pathway, levels related adipocyte differentiation, including CD36, RXRα, RXRγ, FABP4, PLIN1, ADIPO, CAP, upregulated (P < 0.01). blot ELISA pathway showed MGP increased proteins indicating possible activation summary, may promote by thereby activating

Language: Английский

Citations

0
Virtual screening combined with molecular docking for the !identification of new anti-adipogenic compounds DOI Creative Commons
Gilberto Mandujano-Lázaro, María F. Torres-Rojas, Esther Ramírez‐Moreno

et al.

Science Progress, Journal Year: 2025, Volume and Issue: 108(1)

Published: Jan. 1, 2025

Obesity is an important risk factor for diabetes, cardiovascular diseases, and cancer, reducing the quality of life expectancy millions people. Consequently, obesity has turned into one most health public problems worldwide, which highlights urgent need new safe treatments. mainly related to excessive fat accumulation; therefore, proteins participating in white adipose tissue increase dysfunction are considered pertinent attractive targets developing methods that can help with body weight control. In this context, virtual screening libraries containing a large number molecules represents valuable strategy identification potential anti-adipogenic compounds reduced costs time production. Here, we review scientific literature about prediction ligands specific through molecular docking chemical libraries, aim proposing molecules. First, present adipogenesis adipocyte functions were selected following studies: PPARγ, Crif1, SIRT1, ERβ, PC1, FTO, Mss51, FABP4. Then, describe obtention according characteristics approach, i.e. structure-based drug design (SBDD) or ligand-based (LBDD). Finally, critical analysis these computational strategies corresponding results points out necessity combining vitro vivo assays effective It also evidences translating successful candidates control remains huge challenge.

Language: Английский

Citations

0
Paeoniflorin: a review of its pharmacology, pharmacokinetics and toxicity in diabetes DOI Creative Commons

Xue Ou,

Zhijie Yu,

Chun Pan

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 7, 2025

The escalating global prevalence of diabetes underscores the urgency addressing its treatment and associated complications. Paeoniflorin, a monoterpenoid glycoside compound, has garnered substantial attention in recent years owing to potential therapeutic efficacy management. Thus, this study aims systematically overview pharmacological effects, pharmacokinetics toxicity paeoniflorin diabetes. Plenty evidences have verified that improves complication through reducing blood sugar, enhancing insulin sensitivity, regulating gut microbiota autophagy, restoration mitochondrial function, regulation lipid metabolism, anti-inflammation, anti-oxidative stress, inhibition apoptosis, immune so on. Paeoniflorin possess characteristics rapid absorption, wide distribution, metabolism renal excretion. Meanwhile, studies suggested low acute toxicity, minimal subacute chronic no genotoxic or mutational toxic effects. In conclusion, paper elucidates application safety profile

Language: Английский

Citations

0
Comprehensive analysis of Syzygium cumini L. pomace extract as an α-amylase inhibitor: In vitro inhibition, kinetics, and computational studies DOI

Venkatramanan Varadharajan,

Ashwath Kumar Balu,

Bruce Joshua Sinclair

et al.

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108498 - 108498

Published: May 1, 2025

Language: Английский

Citations

0
Microbe-derived Antioxidants Enhance Lipid Synthesis by Regulating the Hepatic AMPKα–SREBP1c Pathway in Weanling Piglets DOI Creative Commons
Chengbing Yu, Di Wang, Cheng Shen

et al.

Journal of Nutrition, Journal Year: 2024, Volume and Issue: 154(4), P. 1101 - 1108

Published: Feb. 9, 2024

Language: Английский

Citations

3
PPARG modulation by bioactive compounds from Salvia officinalis and Glycyrrhiza glabra in type 2 diabetes management: A in silico study DOI
Saeideh Hoseinpoor, Jamshidkhan Chamani,

Mohammad-Reza Saberi

et al.

Innovation and Emerging Technologies, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 1, 2024

This study delves into the exploration of bioactive constituents derived from Salvia officinalis and Glycyrrhiza glabra, with a focus on their potential to modulate peroxisome proliferator-activated receptor gamma (PPARG), crucial regulator glucose homeostasis key target for diabetes management. Through rigorous application in silico methodologies, interactions between PPARG six natural compounds, including kanzonol X, glabrol, linolenic acid, glycyrrhizin, carnosic licoflavone A, were meticulously examined. Molecular docking studies unveiled notable binding affinity X glabrol receptor, indicating effectiveness regulating blood levels through activation. dynamics simulations affirmed stability PPARG-ligand complexes, highlighting significance hydrogen bonds maintaining stability. Furthermore, computational techniques utilized assess absorption, distribution, metabolism, excretion (ADME) properties as well compounds’ cardiotoxicity. Encouragingly, these substances exhibited efficient cellular uptake, favorable bioavailability, demonstrated no cardiotoxic effects. Kanzonol abundant G. have emerged strong candidates regulation Although methodologies offered vital insights, unlocking full compounds necessitates research clinical trials validate safety managing this patient population.

Language: Английский

Citations

3