Frontiers in Medicine,
Journal Year:
2024,
Volume and Issue:
11
Published: Dec. 6, 2024
Medicinal
plants,
also
known
as
herbs,
have
been
discovered
and
utilized
in
traditional
medical
practice
since
prehistoric
times.
plants
proven
rich
thousands
of
natural
products
that
hold
great
potential
for
the
development
new
drugs.
Previously,
we
reviewed
types
Chinese
medicines
a
Tang
Dynasty
monk
Jianzhen
(Japanese:
Ganjin)
brought
to
Japan
from
China
742.
This
article
aims
review
origin
Kampo
(Japanese
medicine),
present
overview
neurodegenerative
diseases
retinitis
pigmentosa
well
medicinal
some
depth.
Through
study
history
Kampo,
found
herbs
contain
many
neuroprotective
ingredients.
It
provides
us
perspective
on
extracting
components
treat
diseases.
Retinitis
(one
ophthalmic
diseases)
is
an
incurable
blinding
disease
has
become
popular
research
direction
global
ophthalmology.
To
date,
treatments
are
very
limited
worldwide.
Therefore,
intend
integrate
knowledge
skills
different
disciplines,
such
science,
pharmaceutical
science
plant
take
therapeutic
approach
In
future,
will
use
specific
active
ingredients
extracted
pigmentosa.
By
exploring
potent
bioactive
valuable
opportunity
be
offered
uncover
novel
approaches
drugs
which
target
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Feb. 13, 2025
Abstract
The
aqueous
two-phase
system
(ATPS)
as
an
environmentally
friendly
technique
has
a
high
potential
for
extraction
of
drugs,
proteins,
metals,
etc.
In
this
work
first
the
formation
ATPSs
by
betaine,
which
is
novel,
low
toxic,
and
biodegradable
material,
polyethylene
glycol
or
phosphate
salts
(K
3
PO
4
K
2
HPO
)
were
investigated
temperatures
298.15,
308.15,
318.15
atmospheric
pressure
(≈
85
kPa).
effect
increasing
temperature
on
binodal
curves
was
investigated.
experimental
data
correlated
using
two
empirical
nonlinear
three
parameter
expressions
developed
Merchuk,
Zafarani-Moattar
et
al.
two-parameter
modified
equation
derived
from
effective
excluded
volume
theory.
Then,
tie-line
represented
with
Othmer-Tobias
Setschenow
correlations.
Finally,
in
these
partition
behavior
analgesic
namely
acetaminophen,
salicylic
acid,
ceftriaxone
measuring
coefficient
efficiency.
trend
obtained
results
showed
that
selected
drugs
successfully
extracted
into
top
phase
studied
greater
than
one
salt
systems
efficiency
up
to
98%
case
polymer
8%
at
different
tie-lines
it
found
hydrophobicity
partitioned
responsible
phenomenon.
Molecules,
Journal Year:
2025,
Volume and Issue:
30(2), P. 409 - 409
Published: Jan. 19, 2025
The
separation
of
large
polar
constituents
presents
a
substantial
challenge
in
natural
product
research
when
employing
column
chromatography
techniques,
as
the
process
is
both
complex
and
time-consuming.
In
this
study,
an
acetonitrile/tetrahydrofuran/di-(2-ethylhexyl)
phosphoric
acid/aqueous
saturated
sodium
chloride
solvent
system
was
developed
utilized
for
countercurrent
from
Ginkgo
biloba
L.
seeds.
Five
were
effectively
isolated
using
(2:2:0.8:3,
v/v)
two-step
method.
initial
process,
three
successfully
purified
methanol
extract:
compound
1,
4,
5.
Compounds
2
3,
co-eluted
column,
further
subjected
to
inner-recycling
chromatographic
procedures.
At
last,
five
identified,
including
4′-O-methylpyridoxine
(1);
two
indole
alkaloid
N-glucosides,
ginkgoside
B
(2)
A
(3);
2-(4-hydroxybenzyl)
malic
acid
(4);
coniferyl
alcohol
(5).
results
demonstrated
that
serves
feasible
efficient
components.
Comprehensive Reviews in Food Science and Food Safety,
Journal Year:
2025,
Volume and Issue:
24(2)
Published: March 1, 2025
Abstract
Recently,
the
non‐intestinal
functions
of
human
milk
oligosaccharides
(HMOs)
have
been
widely
documented,
including
their
roles
in
promoting
brain
development
and
growth,
as
well
ameliorating
anxiety,
allergies,
obesity.
Understanding
mechanisms
action
is
becoming
increasingly
critical.
Furthermore,
these
effects
are
frequently
associated
with
type
structure
HMOs.
As
an
innovative
technology,
“plant
factory”
expected
to
complement
traditional
synthesis
technology.
This
study
reviews
novel
techniques.
Particular
emphasis
placed
on
processes,
advantages,
limitations
technology
can
express
genes
related
HMO
instantaneously
plant
leaves,
thereby
enabling
rapid
cost‐effective
generation
However,
remains
underdeveloped,
challenges
low
yield
unsustainable
production
must
be
addressed.
we
present
overview
most
recent
clinical
preclinical
studies
review
emphasizes
underlying
HMOs
primarily
exert
through
cleavage
beneficial
monomer
components,
metabolism
produce
advantageous
metabolites,
regulation
immune
responses.
Phytochemical Analysis,
Journal Year:
2024,
Volume and Issue:
35(3), P. 599 - 616
Published: Jan. 29, 2024
Abstract
Introduction
Accurate
screening
and
targeted
preparative
isolation
of
active
substances
from
natural
medicines
have
long
been
technical
challenges
in
medicine
research.
Objectives
This
study
outlines
a
new
approach
for
improving
the
efficiency
product
preparation,
focusing
on
rapid
accurate
potential
ingredients
Ganoderma
lucidum
efficient
preparation
lipoxidase
inhibitors,
with
aim
providing
ideas
treatment
Alzheimer's
disease
G.
.
Methods
The
medicinal
plant
was
selected
through
ultrafiltration
coupled
liquid
chromatography
mass
spectrometry
(UF–LC–MS)
computer‐assisted
lipoxygenase
(LOX)
inhibitors.
In
addition,
inhibitory
effect
compounds
LOX
studied
using
enzymatic
reaction
kinetics,
underlying
mechanism
is
discussed.
Finally,
based
earlier
activity
guidelines,
identified
ligands
were
isolated
purified
complex
(high‐speed
countercurrent
semi‐preparative
high‐performance
chromatography).
Results
Five
ingredients,
ganoderic
acids
A,
B,
C
2
,
D
F,
We
improved
purity
compound
virtual
computer
enzyme
inhibition
assays
combined
chromatography.
Conclusion
innovative
methods
UF–LC–MS,
computer‐aided
screening,
provide
powerful
tools
separating
inhibitors
matrices
favourable
platform
large‐scale
production
bioactive
nutrients.
Phytochemical Analysis,
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 3, 2024
Abstract
Introduction
Sophora
flavescens
Aiton
(Fabaceae),
a
ubiquitous
plant
species
in
Asia,
contains
wide
range
of
pharmacologically
active
compounds,
such
as
flavonoids,
with
potential
anti‐Alzheimer's
disease
(anti‐AD)
effects.
Objectives
The
objective
the
study
is
to
develop
quaternity
method
for
screening,
isolation,
extraction
optimization,
and
activity
evaluation
acetylcholinesterase
(AChE)‐inhibiting
compounds
from
S.
realize
high‐throughput
screening
substances
traditional
Chinese
medicine
provide
experimental
data
development
anti‐AD
drugs.
Methods
With
AChE
target
molecule,
affinity
ultrafiltration
liquid
chromatography‐mass
spectrometry
were
applied
screen
inhibitors
enzyme
.
Orthogonal
array
experiments
combined
multi‐objective
Non‐Dominated
Sorting
Genetic
Algorithm
III
was
used
first
time
optimize
process
extracting
substances.
Enzyme
inhibition
kinetics
molecular
docking
studies
performed
verify
effects
compounds.
Results
Five
AChE‐inhibiting
identified:
kushenol
I,
kurarinone,
sophoraflavanone
G,
isokurarinone,
E.
These
successfully
separated
at
purities
72.88%,
98.55%,
96.86%,
96.74%,
95.84%,
respectively,
using
n
‐hexane/ethyl
acetate/methanol/water
(4.0/5.0/4.0/5.0,
v/v/v/v),
(5.0/5.0/6.0/4.0,
(4.9/5.1/5.7/4.3,
v/v/v/v)
mobile
phase
systems.
revealed
that
E
had
best
inhibitory
effect.
Conclusion
This
elucidates
mechanism
action
five
provides
theoretical
basis
other
therapeutic
