Effects of poly (ADP‐ribose) polymerase 1 (PARP1) on silk proteins in the silkworm, Bombyx mori DOI
Mingke Wu, Hao Sun,

Aoming Wang

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: 33(6), P. 732 - 743

Published: July 3, 2024

Abstract Animal silk is economically important, while secretion a complex and subtle mechanism regulated by many genes. We identified the poly (ADP‐ribose) polymerase ( PARP1 ) gene of silkworm successfully cloned its coding sequence (CDS) sequence. Using clustered regularly interspaced short palindromic repeat (CRISPR/Cas9) technology, we screened single guide RNA (sgRNA) with high knockout efficiency cellular experiments obtained mutants knocking out at individual level. found that mainly exhibited phenotypes such as smaller cocoon size reduced shell rate than wild type. also detected expression protein genes in mutant quantitative real‐time PCR (qPCR) some was slightly down‐regulated. Meanwhile, together results transcriptomic analysis, hypothesized may affect synthesis proteins, resulting their failure to function properly. Our study provide an important reference for future in‐depth refinement molecular silk‐producing animals, well potential idea development breeding lines silkworms improve production.

Language: Английский

BmNPV interacts with super-enhancer regions of the host chromatin to hijack cellular transcription machinery DOI Creative Commons
Shudi Zhao, Xingyang Wang, Tian Yang

et al.

Nucleic Acids Research, Journal Year: 2025, Volume and Issue: 53(6)

Published: March 20, 2025

Abstract Effective transcriptional activation relies on the spatial interaction between specific DNA elements. interactions have also been observed viruses and their hosts, with limited understanding of involved details. Baculovirus is a representative species virus has reported to interact host genome in our previous study. However, biological significance baculovirus-host trans-species its underlying mechanisms remain elusive. Here, using Bombyx mori nucleopolyhedrovirus (BmNPV) as model virus, we combine epigenome, transcriptome, biochemical assays investigate interaction. Our data show that BmNPV hijacks regulatory capacity super-enhancers (SEs) by physically interacting these regions genome. This results usurpation activating an SE-binding transcription factor GATA thereby impairing SE-induced target antiviral genes. Moreover, hijacked spread through cis-interaction viral DNA, leading enhanced gene expression. Overall, provide novel insights into intricate interplay expression networks broaden vision exploitation cellular machinery.

Language: Английский

Citations

0
Effects of poly (ADP‐ribose) polymerase 1 (PARP1) on silk proteins in the silkworm, Bombyx mori DOI
Mingke Wu, Hao Sun,

Aoming Wang

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: 33(6), P. 732 - 743

Published: July 3, 2024

Abstract Animal silk is economically important, while secretion a complex and subtle mechanism regulated by many genes. We identified the poly (ADP‐ribose) polymerase ( PARP1 ) gene of silkworm successfully cloned its coding sequence (CDS) sequence. Using clustered regularly interspaced short palindromic repeat (CRISPR/Cas9) technology, we screened single guide RNA (sgRNA) with high knockout efficiency cellular experiments obtained mutants knocking out at individual level. found that mainly exhibited phenotypes such as smaller cocoon size reduced shell rate than wild type. also detected expression protein genes in mutant quantitative real‐time PCR (qPCR) some was slightly down‐regulated. Meanwhile, together results transcriptomic analysis, hypothesized may affect synthesis proteins, resulting their failure to function properly. Our study provide an important reference for future in‐depth refinement molecular silk‐producing animals, well potential idea development breeding lines silkworms improve production.

Language: Английский

Citations

0