Biointerfacial Supramolecular Self-Assembly of Whey Protein Isolate Nanofibrils on Bacterial Surface for Improved Survival and Applied to 3d Food Printing DOI
Ye Zhang, Yexuan Wang, Xiaohan Dai

et al.

Published: Jan. 1, 2024

Personalized three-dimensional (3D) printed foods rich in probiotics were investigated. Lactiplantibacillus plantarum (Lp), as a representative of probiotics, was used to investigate the 3D printing probiotic-rich foods. Here, for first time, whey protein isolate nanofibrils (WPNFs) coated and anchored on bacterial surfaces via biointerfacial supramolecular self-assembly, providing protection against environmental stress process. The optimized composite gels consisting HG (0.25 g), WPI (1.25 FOS (0.75 Lp-WPNFs-GTB emulsion (Φ=40%, 3.75 mL) can realize printing, exhibit high resolution, smooth surface, stable shape. viable cell count is higher than 8.0 log CFU/g. They are particularly suitable people with dysphagia classified level 5 IDDSI framework. results provide new insights into development WPNFs-coating deliver food probiotics.

Language: Английский

Rewiring Tryptophan Metabolism via Programmable Probiotic Integrated by Dual-Layered Microcapsule Protects against Inflammatory Bowel Disease in Mice DOI
Wen Li, Yichen Liu, Xiaoming Zheng

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(52), P. 35443 - 35464

Published: Nov. 28, 2024

Intestinal dysbiosis and the associated l-tryptophan metabolic disorder are pivotal in inflammatory bowel disease progression, leading to a compromised intestinal barrier integrity. Remedying dysfunction tryptophan metabolism has emerged as promising therapeutic strategy. Herein, we reprogram situ by EcN-TRP@A/G, encapsulating engineered probiotic, EcN-TRP, with enhanced synthesis capacity, for sustained modulation, thereby restoring function microbial homeostasis. The pH-responsive dual-layered EcN-TRP@A/G microcapsule developed via high-voltage electrospraying liquid interface self-assembly, preserved probiotic viability harsh gastrointestinal milieu, facilitated targeted colon release. Bioluminescent tracking mice reveals 22.84-fold increase distribution compared naked EcN-TRP. Targeted metabolomics highlights EcN-TRP@A/G's modulation of tryptophan-indole pathway. Oral administration elevates indole metabolites, particularly indole-3-acetic acid indole-3-propionic acid, tissue up 7 days. In IBD mice, improves permeability, reduces inflammation, recovers gut microbiome enhancing beneficial bacteria abundance like Prevotellaceae_UCG-001 Anaerostipes while suppressing pathogenic strains Escherichia-Shigella. Our findings offer cost-effective approach, harnessing potential through modifications effective treatment.

Language: Английский

Citations

3

Enhanced viability of Lactiplantibacillus plantarun by encapsulation with whey protein isolate fibrils and polysaccharides through layer-by-layer coating DOI
Siyuan Li, Wei Su, Yu Zhang

et al.

Food Hydrocolloids, Journal Year: 2025, Volume and Issue: unknown, P. 111105 - 111105

Published: Jan. 1, 2025

Language: Английский

Citations

0

Engineering live cell surfaces with polyphenol-functionalized nanoarchitectures DOI Creative Commons
Yunxiang He, Qinling Liu,

Yuanmeng He

et al.

Chemical Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Polyphenol-functionalized nanoarchitectures (cytoPNAs) provide a modular and cell independent strategy to create biohybrids for engineering, with improved production biomanufacturing enhanced efficacy cell-based therapies.

Language: Английский

Citations

0

Liposome-mediated encapsulation of probiotics: current status, challenges and future directions DOI
Jing Liu,

Zhenchun Sun,

Shao‐Quan Liu

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 18

Published: April 3, 2025

Probiotics play a crucial role in maintaining human health, particularly intestinal health. However, their application has been hindered by issues related to stability, survival through the gastrointestinal tract, and effective delivery target sites. Encapsulating probiotics within liposomes not only protects them from environmental factors such as light, oxygen, temperature but also shields harmful influences like gastric acid bile. In addition, encapsulation promotes growth, release, absorption of intestines. This study reviews characteristics advantages, discusses current state knowledge liposomes. Additionally, investigates how digestive process affects performance probiotics, focusing on structural changes that occur both probiotic-loaded Finally, challenges future directions field liposome-delivered probiotics. Liposome represents an innovative technology significantly enhances stability bioavailability thereby offering improved protection for individuals' novel approach creates new possibilities advantages realm health is anticipated receive broader applications future.

Language: Английский

Citations

0

Live Probiotics-Loaded Delivery Systems for the Treatment of Infected Wounds DOI
Oksana Gerulis, Karin Kogermann

NATO science for peace and security series. A, Chemistry and biology, Journal Year: 2025, Volume and Issue: unknown, P. 51 - 78

Published: Jan. 1, 2025

Language: Английский

Citations

0

Research progress regarding physiological functions of probiotics and techniques to enhance their stress resistance: a review DOI
Wei Luo, Ling Yang,

Yangyue Ding

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 29

Published: May 5, 2025

Probiotics have received widespread attention for their unique physiological functions, such as promoting digestion and absorption of nutrients, modulating the immune system, inhibiting pathogenic microorganisms. However, probiotics face severe challenges in terms stability viability practical applications, environmental factors like acid, osmotic pressure, temperature, oxidation bile salts, which make it difficult to maintain sufficient numbers when they are delivered target location. Therefore, some technical protection is needed improve adhesion gastrointestinal tract minimize damage probiotic products during processing, transportation handling. We review underlying mechanisms by exert well various strategies enhance tolerance, especially traditional production methods modification genetic pathways. In addition, application tolerogenic fermented foods clinical medicine discussed. Through in-depth analysis potential function tolerance enhancement, not only provide theoretical support probiotics, but also pave way development functional innovative human disease treatment strategies.

Language: Английский

Citations

0

A review of multimodule stimuli-responsive chitosan-incorporated lipid-based micro/nanocarriers for drug delivery in cancer therapy: Promises, outlooks, and prospects DOI
Zahra Ghasemi, Ali Maleki,

Taha Jafari

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 144587 - 144587

Published: May 1, 2025

Language: Английский

Citations

0

Dual-layer probiotic encapsulation using metal phenolic network with gellan gum-tamarind gum coating for colitis treatment DOI

Xinchuang Wang,

Yu Xu, Wen Jiang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 135759 - 135759

Published: Sept. 1, 2024

Language: Английский

Citations

3

Layer-by-layer concurrent encapsulation of probiotics and bioactive compounds with supplementation in intermediary layers: An establishing instrument for microbiome recharge, core safety, and targeted delivery DOI

Muhammad Safiullah Virk,

Muhammad Abdulrehman Virk,

Mehak Gul

et al.

Food Hydrocolloids, Journal Year: 2024, Volume and Issue: unknown, P. 110873 - 110873

Published: Nov. 1, 2024

Language: Английский

Citations

2

Electrostatic spraying encapsulation of probiotic-loaded W/O/W emulsion in sodium alginate microspheres to enhance probiotic survival stability DOI
Yongchao Wang, Wenhao Ding,

Fengzhi Qiao

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138005 - 138005

Published: Nov. 1, 2024

Language: Английский

Citations

2