SLC30A2-Mediated Zinc Metabolism Modulates Gastric Cancer Progression via the Wnt/β-Catenin Signaling Pathway DOI Creative Commons
Fan Li, Xiaohong Zhang, Li Feng

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(10)

Published: Oct. 12, 2024

Background: Gastric cancer (GC) is a significant global health burden with limited treatment options. The purpose of this study was to investigate the role SLC30A2, zinc transporter, in GC development and its capacity as target for therapy. Methods: A comprehensive analysis datasets (GSE54129 stomach adenocarcinoma (STAD) from Cancer Genome Atlas (TCGA)) conducted using bioinformatics tools examine differential gene expression, focusing on SLC30A2. Functional assays, including Cell counting kit-8 (CCK-8) transwell were carried out cell lines determine impact SLC30A2 knockdown behavior. Flow cytometry utilized quantitatively observe apoptosis cycle progression. sulfate (ZnSO4) cells evaluated by detecting markers, Wnt/β-catenin signaling pathway activity, oxidative stress biomarkers, regulatory effect overexpression. Results: Our revealed upregulation samples compared normal samples, high expression linked poor prognosis. repressed proliferation, invasion, migration cells, induced apoptosis, well arrested cycle. Additionally, ZnSO4 cytotoxicity while overexpression rescued ZnSO4-induced, migration, proliferation. Moreover, had been shown bolster trigger pathway, effects which mitigated Conclusion: results implied that essential progression modulating homeostasis cellular processes. Targeting or may represent potential therapeutic approach treatment.

Language: Английский

Mechanistic insights into CDCA gene family-mediated glioblastoma progression: implications for diagnosis, prognosis, and therapeutic targeting DOI Creative Commons
Chang Liu

Hereditas, Journal Year: 2025, Volume and Issue: 162(1)

Published: March 20, 2025

Abstract Background Glioblastoma (GBM) is a highly aggressive brain tumor characterized by poor prognosis and limited therapeutic options. Understanding the molecular mechanisms driving GBM progression essential for developing more effective diagnostic approaches. Specifically, investigating Cell Division Cycle-Associated (CDCA) genes offers new perspectives on cell cycle regulation proliferation of cells, which are key factors in growth resistance to treatment. These have not been extensively studied GBM, making them promising area targeted research potential interventions. This project was launched elucidate pathogenic, diagnostic, roles CDCA GBM. Methodology Total RNA extracted from lines followed RT-qPCR analyze expression genes. The validation, prognostic significance, mutational analysis were performed using various databases. Functional assays, including gene knockdown, colony formation, proliferation, wound healing, conducted U87MG cells assess role CDCA7 CDCA8 Results 12 6 normal revealed significant overexpression these ROC curve demonstrated excellent potential, with AUC values 1 most indicates that effectively distinguishes cells. Validation additional TCGA data confirmed upregulation tumors, association cancer-related pathways. Survival showed higher correlated patients. Mutation, CNV, methylation analyses alterations genes, further supporting their Additionally, linked immune modulation cycle-related functions, suggesting involvement evasion proliferation. Knockdown experiments reduction migration, highlighting as targets. Conclusion Overall, our findings suggest could serve both biomarkers targets

Language: Английский

Citations

0
Pharmacological landscape of endoplasmic reticulum stress: uncovering therapeutic avenues for metabolic diseases DOI
Ghallab Alotaibi,

Abdullah Alkhammash

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 177509 - 177509

Published: March 1, 2025

Language: Английский

Citations

0
Identification and functional characterization of key biomarkers in diffuse large B-cell lymphoma: emphasis on STYX as a prognostic marker and therapeutic target DOI Creative Commons
Junaid Abid,

Basil A. Mahmood Al-Rawi,

Ahmad Mahmood

et al.

Hereditas, Journal Year: 2025, Volume and Issue: 162(1)

Published: March 24, 2025

Abstract Diffuse large B-cell lymphoma (DLBC) is the most common subtype of non-Hodgkin lymphoma, characterized by its aggressive nature and poor prognosis in advanced stages. Despite advances treatment, molecular mechanisms driving DLBC progression remain incompletely understood, necessitating identification novel biomarkers for diagnosis prognosis. In this study, we analyzed two publicly available datasets (GSE32018 GSE56315) from Gene Expression Omnibus database (GEO) to identify overlapping differentially expressed genes (DEGs). Later on, a comprehensive silico vitro methodology was adopted decipher role DEGs DLBC. analysis GSE32018 GSE56315 identified five gene: SP3, CSNK1A1, STYX, SIRT5, MGEA5. validation using GEPIA2 confirmed upregulation downregulation MGEA5 tissues compared normal controls. Furthermore, mutational revealed that CSNK1A1 only gene among these exhibit mutations, with 2.7% mutation frequency patients. Methylation highlighted negative correlation between methylation levels mRNA expression, while survival high STYX expression as significantly associated poorer overall Functional assays demonstrated knockdown U2932 cells led reduced cell proliferation, colony formation, enhanced wound healing, indicating STYX’s pivotal migration. Additionally, enrichment involvement key biological processes, including intracellular trafficking myeloid progenitor differentiation. These findings emphasize potential therapeutic targets DLBC, particularly highlighting promising prognostic marker target intervention.

Language: Английский

Citations

0
Roles of anoikis in hepatocellular carcinoma therapy and the assessment of anoikis-regulatory molecules as therapeutic targets DOI
Hongyu Wang, Ya‐Wen Yang, Gan Zhang

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Language: Английский

Citations

0
Targeting p53-p21 signaling to enhance mesenchymal stem cell regenerative potential DOI
Ahsas Goyal, Muhammad Afzal, Nawaid Hussain Khan

et al.

Regenerative Therapy, Journal Year: 2025, Volume and Issue: 29, P. 352 - 363

Published: April 8, 2025

Language: Английский

Citations

0
SLC30A2-Mediated Zinc Metabolism Modulates Gastric Cancer Progression via the Wnt/β-Catenin Signaling Pathway DOI Creative Commons
Fan Li, Xiaohong Zhang, Li Feng

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(10)

Published: Oct. 12, 2024

Background: Gastric cancer (GC) is a significant global health burden with limited treatment options. The purpose of this study was to investigate the role SLC30A2, zinc transporter, in GC development and its capacity as target for therapy. Methods: A comprehensive analysis datasets (GSE54129 stomach adenocarcinoma (STAD) from Cancer Genome Atlas (TCGA)) conducted using bioinformatics tools examine differential gene expression, focusing on SLC30A2. Functional assays, including Cell counting kit-8 (CCK-8) transwell were carried out cell lines determine impact SLC30A2 knockdown behavior. Flow cytometry utilized quantitatively observe apoptosis cycle progression. sulfate (ZnSO4) cells evaluated by detecting markers, Wnt/β-catenin signaling pathway activity, oxidative stress biomarkers, regulatory effect overexpression. Results: Our revealed upregulation samples compared normal samples, high expression linked poor prognosis. repressed proliferation, invasion, migration cells, induced apoptosis, well arrested cycle. Additionally, ZnSO4 cytotoxicity while overexpression rescued ZnSO4-induced, migration, proliferation. Moreover, had been shown bolster trigger pathway, effects which mitigated Conclusion: results implied that essential progression modulating homeostasis cellular processes. Targeting or may represent potential therapeutic approach treatment.

Language: Английский

Citations

0