Nitric Oxide-Releasing Mesoporous Hollow Cerium Oxide Nanozyme-Based Hydrogel Synergizes with Neural Stem Cell for Spinal Cord Injury Repair DOI
Dun Liu,

Runyan Niu,

Siliang Wang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 19(2), P. 2591 - 2614

Published: Dec. 26, 2024

Neural stem cell (NSCs) transplantation is a promising therapeutic strategy for spinal cord injury (SCI), but its efficacy greatly limited by the local inhibitory microenvironment. In this study, based on l-arginine (l-Arg)-loaded mesoporous hollow cerium oxide (AhCeO2) nanospheres, we constructed an injectable composite hydrogel (AhCeO2-Gel) with microenvironment modulation capability. AhCeO2-Gel protected NSCs from oxidative damage eliminating excess reactive oxygen species while continuously delivering Nitric Oxide to lesion of SCI in pathological microenvironment, latter which effectively promoted neural differentiation NSCs. The process was confirmed be closely related up-regulation cAMP-PKA pathway after NO-induced calcium ion influx. addition, significantly polarization microglia toward M2 subtype as well enhanced regeneration nerves and myelinated axons. prepared bioactive system also efficiently facilitated integration transplanted host circuits, replenished damaged neurons, alleviated neuroinflammation, inhibited glial scar formation, thus accelerating recovery motor function rats. Therefore, synergized has great potential integrated treat comprehensively reversing

Language: Английский

Infertility treatment using polysaccharides-based hydrogels: new strategies in tissue engineering and regenerative medicine DOI Creative Commons
Maryam Ghahremani‐Nasab, Soraya Babaie,

Sara Bazdar

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 4, 2025

Infertility is a primary health issue affecting about 15% of couples reproductive ages worldwide, leading to physical, mental, and social challenges. Advances in nanobiotechnology regenerative medicine are opening new therapeutic horizons for infertility by developing polysaccharide-based nanostructured biomaterials. This review explores the role tissue engineering treatment, explicitly focusing on promising potential hydrogels. In this context, using these biomaterials offers unique advantages, including biodegradability, biocompatibility, ability mimic natural endometrial microenvironment, making them highly effective applications regeneration, ovarian engineering, spermatogenesis support, controlled drug delivery. discusses various properties uses hydrogels, like alginate, hyaluronic acid, chitosan, helping restore function. While materials hold great promise, some notable challenges their clinical use include issues rapid degradation, mechanical instability, immune reactions. Future research should focus hybrid investigating advanced fabrication techniques, testing settings. By combining findings from recent studies, aims provide solid foundation researchers clinicians looking discover strategies treating infertility, ultimately connecting efforts with practical healthcare.

Language: Английский

Citations

0
Photocurable Reduced Graphene Oxide Containing Vitamin E-Loaded Dextran Based Cardiac Hydrogel Patches: Synthesis, Physical Characterization and In Vitro Assessment DOI

Esra Pervin Kızılcaoğlu,

Bengi Özkahraman

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106796 - 106796

Published: March 1, 2025

Language: Английский

Citations

0
Hydrogel encapsulation facilitates a low-concentration cryoprotectant for cryopreservation of mouse testicular tissue DOI
Jia Tan,

Shuqin Jia,

Qiang Xu

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2024, Volume and Issue: 242, P. 114096 - 114096

Published: July 14, 2024

Language: Английский

Citations

2
Biomedical applications and nutritional value of specific food-derived polysaccharide-based hydrogels DOI Creative Commons

Qianru Xiang,

Yuting Hao,

Zijun Xia

et al.

Advances in Nutrition, Journal Year: 2024, Volume and Issue: unknown, P. 100309 - 100309

Published: Sept. 1, 2024

Language: Английский

Citations

2
Structural, mechanical, and cytocompatibility characteristics of hybrid scaffolds from chitosan/decellularized testicular ECM DOI

Maedeh Hamzeh,

Mansoureh Movahedin, Fariba Ganji

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 137908 - 137908

Published: Nov. 1, 2024

Language: Английский

Citations

1
Generation of mouse testicular organoids with highly compartmentalized tubular lumen structure and their cryopreservation DOI
Jia Tan, Jiahui Li,

Chun-Yan Lin

et al.

Life Sciences, Journal Year: 2024, Volume and Issue: 355, P. 122980 - 122980

Published: Aug. 13, 2024

Language: Английский

Citations

0
Transcriptomic Differences by RNA Sequencing for Evaluation of New Method for Long-Time In Vitro Culture of Cryopreserved Testicular Tissue for Oncologic Patients DOI Creative Commons
Cheng Pei, Plamen Todorov,

Qingduo Kong

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(18), P. 1539 - 1539

Published: Sept. 13, 2024

Earlier studies have established that culturing human ovarian tissue in a 3D system with small amount of soluble Matrigel (a basement membrane protein) for 7 days vitro increased gene fusion and alternative splicing events, cellular functions, potentially impacted expression. However, this method was not suitable culture testicular tissue.

Language: Английский

Citations

0
Nitric Oxide-Releasing Mesoporous Hollow Cerium Oxide Nanozyme-Based Hydrogel Synergizes with Neural Stem Cell for Spinal Cord Injury Repair DOI
Dun Liu,

Runyan Niu,

Siliang Wang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 19(2), P. 2591 - 2614

Published: Dec. 26, 2024

Neural stem cell (NSCs) transplantation is a promising therapeutic strategy for spinal cord injury (SCI), but its efficacy greatly limited by the local inhibitory microenvironment. In this study, based on l-arginine (l-Arg)-loaded mesoporous hollow cerium oxide (AhCeO2) nanospheres, we constructed an injectable composite hydrogel (AhCeO2-Gel) with microenvironment modulation capability. AhCeO2-Gel protected NSCs from oxidative damage eliminating excess reactive oxygen species while continuously delivering Nitric Oxide to lesion of SCI in pathological microenvironment, latter which effectively promoted neural differentiation NSCs. The process was confirmed be closely related up-regulation cAMP-PKA pathway after NO-induced calcium ion influx. addition, significantly polarization microglia toward M2 subtype as well enhanced regeneration nerves and myelinated axons. prepared bioactive system also efficiently facilitated integration transplanted host circuits, replenished damaged neurons, alleviated neuroinflammation, inhibited glial scar formation, thus accelerating recovery motor function rats. Therefore, synergized has great potential integrated treat comprehensively reversing

Language: Английский

Citations

0