Frontiers in Bioscience-Landmark,
Journal Year:
2024,
Volume and Issue:
29(5)
Published: May 14, 2024
Plant
diseases
caused
by
pathogens
pose
significant
threats
to
agricultural
productivity
and
food
security
worldwide.
The
traditional
approach
of
relying
on
chemical
pesticides
for
disease
management
has
proven
be
unsustainable,
emphasizing
the
urgent
need
sustainable
environmentally
friendly
alternatives.
One
promising
strategy
is
enhance
plant
resistance
against
through
various
methods.
This
review
aims
unveil
explore
effective
methods
stimulating
resistance,
transforming
vulnerable
plants
into
vigilant
defenders
pathogens.
We
discuss
both
conventional
innovative
approaches,
including
genetic
engineering,
induced
systemic
(ISR),
priming,
use
natural
compounds.
Furthermore,
we
analyze
underlying
mechanisms
involved
in
these
methods,
highlighting
their
potential
advantages
limitations.
Through
an
understanding
scientists
agronomists
can
develop
novel
strategies
combat
effectively
while
minimizing
environmental
impact.
Ultimately,
this
research
offers
valuable
insights
harnessing
plant’s
innate
defense
paves
way
practices
agriculture.
AMB Express,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Jan. 4, 2025
Abstract
A
Novel
cold-active
chitin
deacetylase
from
Shewanella
psychrophila
WP2
(SpsCDA)
was
overexpressed
in
Escherichia
coli
BL21
and
employed
for
deacetylation
of
to
chitosan.
The
produced
chitosan
characterized,
its
antifungal
activity
investigated
against
Fusarium
oxysporum
.
purified
recombinant
SpsCDA
appeared
as
a
single
band
on
SDS-PAGE
at
approximately
60
kDa,
specific
92
U/mg.
optimum
temperature
pH
were
15
°C
8.0,
respectively,
the
enzyme
significantly
enhanced
presence
NaCl.
bioconversion
by
accomplished
72
h,
yield
69.2%.
solubility
estimated
be
73.4%,
degree
78.1%.
molecular
weight
224.7
±
8.4
kDa
with
crystallinity
index
(CrI)
value
18.75.
Moreover,
FTIR
XRD
spectra
revealed
characteristic
peaks
enzymatically
compared
standard
chitosan,
indicating
their
structural
similarity.
inhibited
spore
germination
F.
minimum
inhibitory
concentration
(MIC)
1.56
mg/mL.
potential
effect
is
attributed
inhibition
accompanied
ultrastructural
damage
membranes
leakage
cellular
components,
evidenced
transmission
electron
microscopy
(TEM),
accumulation
reactive
oxygen
species
(ROS)
that
confirmed
fluorescence
microscopy.
This
study
shed
light
S.
provides
candidate
green
preparation
