Exploring the potential of melittin peptide: Expression, purification, anti-pathogenic properties, and promising applications as a bio-preservative for beef slices

LWT, Journal Year: 2024, Volume and Issue: 199, P. 116083 - 116083

Published: April 15, 2024

Melittin peptide is a short cationic linear derived from honey bee venom with 26 amino acids, demonstrates potential in controlling and eliminating pathogens. In this study, the genetic sequence of melittin was inserted into pcDNA3.1+ plasmid expressed HEK-293 cells His-tag. Purification done through affinity chromatography nickel column. antimicrobial effect beef sirloin first investigated, minimum inhibitory concentration (MIC) determined. Then, combining it nanochitosan (n-CH) coating, (0.1% 0.2%v/v), alone on physicochemical, microbiological, sensory properties examined over 9-day at 4°C. At end storage period, coated samples effectively prevented an increase pH (5.75±0.06) showed significant decrease peroxide value (PV) (4.50±0.07). The most favorable result observed when using n-CH+0.2%M, which led to malondialdehyde level 0.44±0.0.05 mg/kg amount thiobarbituric acid reactant. Additionally, total volatile base nitrogen content treatment involving 0.2%M n-CH+0.2% 0.1%M deemed acceptable. Although viable count (TVC) microorganisms covered increased time (7.88±0.12 CFU/g), microbial growth rate these lower than control sample (12.10±0.13 CFU/g). Therefore, use based edible coatings combination meat packaging reduces population increases its shelf life.

Language: Английский

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Biopolymer-supported thin-film composite membranes for reverse osmosis

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159264 - 159264

Published: Jan. 1, 2025

Language: Английский

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Progress on layered double hydroxides as green materials in sustainable agricultural production

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121031 - 121031

Published: Feb. 1, 2025

Language: Английский

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Chitin and chitosan from shellfish waste and their applications in agriculture and biotechnology industries

Critical Reviews in Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 19

Published: March 16, 2025

A shellfish processing plant generates only 30–40% of edible meat, while 70–60% portions are considered inedible or by-products. This large amount byproduct waste contains 20–40% chitin, that can be extracted using chemical greener alternative extraction technologies. Chitin and its derivative (chitosan) natural polysaccharides with nontoxicity, biocompatible, biodegradable properties. Due to their versatile physicochemical, mechanical, various bioactivities, these compounds find applications in industries, including: biomedical, dental, cosmetics, food, textiles, agriculture, biotechnology. In the agricultural sector, have been reported promote: growth, defense system, slow release nutrients fertilizer, nutrition, remediate soil conditions, etc. Whereas, biotechnology indicated: enhanced enzyme stability efficacy, water purification remediation, application fuel cells supercapacitors for energy conversion, acting as a catalyst synthesis, review provides comprehensive discussion on utilization biopolymers agriculture (fertilizer, seed coating, treatment, bioremediation) (enzyme immobilization, wastewater synthesis). Additionally, techniques including conventional non-thermal reported. Lastly, concluding remarks future direction provided.

Language: Английский

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Chitosan as a multifunctional polymer in drug delivery systems for diabetic wound management

Polymer Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Language: Английский

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Recent progress of functional nano-chitosan in pharmaceutical and biomedical applications: An updated review

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138324 - 138324

Published: Dec. 1, 2024

Language: Английский

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