Gut microbial dysbiosis exacerbates long-term cognitive impairments by promoting intestinal dysfunction and neuroinflammation following neonatal hypoxia-ischemia

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: Feb. 26, 2025

Neonatal hypoxic-ischemic brain damage (HIBD) is considered as a major cause of long-term cognitive impairments in newborns. It has been demonstrated that gut microbiota closely associated with the prognosis various neurological disorders. However, role microbiota-gut-brain axis on function following neonatal HIBD remains elusive. In this experiment, correlation analysis supported involvement microbial changes (HI) insult development impairments. Subsequent experiment revealed intestinal dysfunction hippocampal neuroinflammation and synaptic injury. causal relationship validation experiments, fecal transplantation (FMT) from cognitively normal rats could restore composition, improve dysfunction, reduce serum levels lipopolysaccharides (LPS) inflammatory mediators, alleviate neuroinflammation, recipient rats. Conversely, FMT induce above adverse pathological Moreover, oral administration anti-inflammatory agent dexamethasone (DEX) exhibited potential to these detrimental effects rats, efficacy being partly reliant microbiota. Further molecular mechanisms using RNA sequencing indicated significant increase toll-like receptor 4 (TLR4) gene tissues Additionally, interventions such TLR4 inhibitor TLR4-IN-C34 administration, FMT, DEX were modulate by inhibiting LPS/TLR4 signaling pathway, thereby exerting neuroprotective effects. Collectively, findings underscore contribution dysbiosis post HI activating triggering inflammation exacerbating systemic inflammation, consequently worsening Hence, rectifying or regulating may represent promising strategy for alleviating neonates affected HIBD.

Language: Английский

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Ultra-small starch microspheres with narrow size distribution prepared in aqueous two-phase system of starch-PVP

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141544 - 141544

Published: Feb. 28, 2025

Language: Английский

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ROS-Responsive Cationic Polymers with Intrinsic Anti-Inflammatory Activity for Intracellular Protein Delivery

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

The intracellular delivery of protein drugs via nanocarriers offers significant potential for expanding their therapeutic applications. However, the unintended activation innate immune responses and inflammation triggered by carriers presents a major challenge, often compromising efficacy. Here, we present oligoethylenimine-thioketal (OEI-TK), reactive oxygen species-responsive cationic polymer with intrinsic anti-inflammatory properties, to overcome this challenge. OEI-TK self-assembles electrostatically bovine serum albumin (BSA) form stable nanoparticles (OTB NPs) excellent encapsulation efficiency. In vitro studies confirmed that OTB NPs retained OEI-TK's antioxidant enhanced biocompatibility, efficiently delivered BSA into cells. Furthermore, facilitated β-galactosidase while preserving its enzymatic activity, demonstrating functional transport. These findings highlight as promising platform dual benefits modulation delivery, holding synergistic treatment inflammation-related diseases.

Language: Английский

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Exochorda racemosa attenuates DSS-induced colitis in C57BL/6J mice by regulating inflammatory factors, reducing oxidative stress, and modulating intestinal flora

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156768 - 156768

Published: April 1, 2025

Language: Английский

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Exploring starch-based excipients in pharmaceutical formulations: Versatile applications and future perspectives

European Journal of Pharmaceutics and Biopharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 114727 - 114727

Published: April 1, 2025

Language: Английский

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Antioxidant Therapy in Inflammatory Bowel Diseases: How Far Have We Come and How Close Are We?

Antioxidants, Journal Year: 2024, Volume and Issue: 13(11), P. 1369 - 1369

Published: Nov. 8, 2024

Inflammatory bowel diseases (IBD) pose a growing public health challenge with unclear etiology and limited efficacy of traditional pharmacological treatments. Alternative therapies, particularly antioxidants, have gained scientific interest. This systematic review analyzed studies from MEDLINE, Cochrane, Web Science, EMBASE, Scopus using keywords like “Inflammatory Bowel Diseases” “Antioxidants.” Initially, 925 publications were identified, after applying inclusion/exclusion criteria—covering July 2015 to June 2024 murine models or clinical trials in humans evaluating natural synthetic substances affecting oxidative stress markers—368 articles included. comprised 344 animal 24 human studies. The most investigated antioxidants polyphenols active compounds medicinal plants (n = 242; 70.3%). found strong link between inflammation IBD, especially on nuclear factor kappa B erythroid 2-related 2 pathways. However, it remains whether occurs first IBD. Lipid peroxidation was the studied damage, followed by DNA damage. Protein damage rarely investigated. relationship gut microbiota examined 103 Human markers scarce, reflecting major research gap IBD treatment. PROSPERO registration: CDR42022335357 CRD42022304540.

Language: Английский

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Improving the prebiotic activity and oxidative stability of carboxymethyl curdlan − quercetin conjugates stabilized Pickering emulsions for the colonic targeting delivery of curcumin

Food Research International, Journal Year: 2024, Volume and Issue: 201, P. 115641 - 115641

Published: Dec. 30, 2024

Language: Английский

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