Review on bioproduction of sialylated human milk oligosaccharides: Synthesis methods, physiologic functions, and applications DOI
Chenchen Li, Zhu Liu, Mengli Li

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 352, P. 123177 - 123177

Published: Dec. 25, 2024

Language: Английский

Preparation of mixed-mode hydrophilic particle for efficient separation of common human milk oligosaccharides DOI

Jingwei Kou,

Han Guo, Jing Leng

et al.

Journal of Chromatography A, Journal Year: 2025, Volume and Issue: 1748, P. 465865 - 465865

Published: March 11, 2025

Language: Английский

Citations

1
Design of a diblock-based membraneless organelle system for metabolic process control DOI
Juntao Ke, Li Wan, Mingqiang Chen

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160239 - 160239

Published: Feb. 1, 2025

Language: Английский

Citations

0
Highly Efficient In Vivo Production of Sialyllacto-N-tetraose C via Screening of Beneficial β1,4-galactosyltransferase and α2,6-sialyltransferase DOI
Yuanlin Liu,

Qian Lin,

Mian Sheng

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: 73(9), P. 5376 - 5384

Published: Feb. 18, 2025

Biological production of human milk oligosaccharides (HMOs) using metabolically engineered strains is a research hotspot in food biotechnology, but less effort has been made on the biological sialylated complex HMOs. Sialyllacto-N-tetraose c only monosialylated HMO top 15 In this study, metabolic pathway LST was constructed Escherichia coli BL21(DE3) by introducing three sequential glycosyltransferases: β1,3-N-acetylglucosaminyltransferase, β1,4-galactosyltransferase, and α2,6-sialyltransferase. The cytidine 5′-monophospho (CMP)-N-acetylneuraminic acid (Neu5Ac) enhanced to improve production. β1,4-galactosyltransferase from Helicobacter pylori J99 (HpGalT) α2,6-sialyltransferase Vespertiliibacter pulmonis (ED6ST) were screened as pair key glycosyltransferases for enhancing final strain could produce 1.718 9.745 g/L shake-flask fed-batch cultivation, respectively, indicating feasibility efficient biosynthesis

Language: Английский

Citations

0
Improving 3′-sialyllactose biosynthesis in Escherichia coli by engineering Neisseria meningitidis 406Y α2,3-sialyltransferase DOI

Qian Lin,

Yuanlin Liu, Hao Wang

et al.

Process Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Regulation of Metabolic Pathways to Enhance Difucosyllactose Biosynthesis in Escherichia coli DOI

Shanquan Liang,

Qi Quan,

Dan Liu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 19, 2024

Difucosyllactose (DFL), an important kind of fucosylated human milk oligosaccharides (HMOs), has garnered considerable attention due to its excellent physiological activities in infants. Previously, we achieved de novo biosynthesis DFL; however, substantial residual intermediates fucosyllactoses (FL) were detected. In this study, DFL was optimized, and FL reduced by regulating metabolic pathways. Different plasmid combinations used regulate gene expression, achieving optimal flux balance between 2′-FL DFL. The expression level key enzyme α-1,3-fucosyltransferase (α-1,3-FT, FucTa) then enhanced increasing copy number integrating fucTa into the chromosome. Exocytosis deleting sugar efflux transporter setA gene, thereby minimizing FL. Finally, strain BSF41 produced 55.3 g/L with only 2.59 a 5 L fermentor, representing highest reported titer date. This study provides foundation for advancing HMOs.

Language: Английский

Citations

1
Review on bioproduction of sialylated human milk oligosaccharides: Synthesis methods, physiologic functions, and applications DOI
Chenchen Li, Zhu Liu, Mengli Li

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 352, P. 123177 - 123177

Published: Dec. 25, 2024

Language: Английский

Citations

1