Food Chemistry X,
Journal Year:
2025,
Volume and Issue:
26, P. 102265 - 102265
Published: Feb. 1, 2025
This
study
employed
a
sophisticated
approach
consisting
of
ultrasound-assisted
enzyme
treatment
to
extract
polysaccharides
from
sea
buckthorn
(SBP).
The
SBP
extraction
parameters
were
optimized,
the
following
optimal
identified:
solid-liquid
ratio
1:32
g/mL,
ultrasound
duration
26
min,
temperature
52
°C,
and
composite
concentration
6000
U/100
mL,
maximum
yield
was
24.07
±
0.15
%.
separation
purification
resulted
in
isolation
three
fractions
(SBPR-1,
SBPR-2,
SBPR-3).
composition
structural
characteristics
SBPRs
identified.
Furthermore,
exhibited
characteristic
absorption
peaks
polysaccharides.
Notably,
surface
microstructures
showed
significant
variations.
Moreover,
all
demonstrated
commendable
thermal
stability
vitro
antioxidant
activity.
serves
as
reference
for
development
application
natural
antioxidants
provides
theoretical
foundation
environmentally
friendly
effective
SBP.
Molecules,
Journal Year:
2025,
Volume and Issue:
30(4), P. 830 - 830
Published: Feb. 11, 2025
This
study
optimized
the
extraction
process
of
crude
polysaccharides
from
Panax
notoginseng
leaves
(PNLP)
using
ultrasonic-assisted
dual-enzyme
method
through
a
single-factor
combined
with
response
surface
experiment.
The
were
subsequently
purified
and
isolated
DEAE-Cellulose
52,
followed
by
structural
analysis,
evaluation
antioxidant
activity,
examination
digestive
enzyme
inhibition.
hypoglycemic
effects
components
further
clarified.
results
indicated
that
polysaccharide
had
an
yield
17.13
±
0.29%.
fraction
PNLP-3
(eluted
0.3
M
NaCl)
was
obtained
52
chromatography,
exhibiting
total
sugar
content
81.2%
molecular
weight
16.57
kDa.
PNLP
is
primarily
composed
arabinose,
galactose,
galacturonic
acid,
molar
percentages
20.24%,
33.54%,
24.27%,
respectively.
mainly
arabinose
29.97%
49.35%,
In
this
IC50
values
for
α-glucosidase
α-amylase
inhibition
1.045
mg/mL
9.53
mg/mL,
Molecular
docking
confirmed
exhibits
better
inhibitory
activity
against
α-glucosidase.
Furthermore,
alleviated
hyperglycemia
in
insulin-resistant
HepG2
cells
enhancing
glucose
consumption
glycogen
synthesis.
exhibited
positive
correlation
its
concentration,
potentially
contributing
to
reducing
oxidative
stress.
These
findings
underscore
therapeutic
potential
leaf
managing
type
2
diabetes
offer
new
perspectives
on
use
natural
regulating
blood
glucose.
A
novel
natural
polysaccharide
(LSP)
was
isolated
and
purified
from
Lactuca
sativa
L.
The
structure
of
LSP
characterized
using
various
techniques,
including
FT-IR,
IC,
Congo
red,
XRD,
DSC,
SEM,
AFM,
NMR.
Furthermore,
its
in
vivo
hypoglycemic
activity
as
an
adjuvant
studied
a
diabetic
mouse
model
induced
by
HFD/STZ.
findings
revealed
that
possesses
molecular
weight
1.3
×
10⁶
kDa,
with
primary
structural
elements
identified
→4)-α-D-Glcp-(1→,
→4,6)-α-Glcp-(1→,
α-Araf-(1→,
→6)-β-D-Galp-(1→,
→3,4)-β-Xylp-(1→,
→4)-β-D-GalpA-(1→.
contains
Ara,
Gal,
Glc,
Xyl
GalA
molar
ratio
0.44:1:2.07:2.11:0.77.
is
semi-crystalline
polymer
lacking
triple
helix
exhibiting
stable
thermal
properties
below
200°C.
Studies
on
demonstrated
the
conjunction
metformin
significantly
improved
effects,
decreased
body
weight,
boosted
antioxidant
capacity,
enhanced
lipid
profiles,
insulin
sensitivity,
hepatic
glycolysis-related
enzyme
activities
mice
compared
to
alone.
This
research
presents
conceptual
rationale
for
exploring
function
polysaccharides,
highlighting
their
potential
adjunctive
role
control
diabetes
mellitus.
