Enhancing Enzymatic Biodiesel Production Using Low-Cost CO₂-Based Alkyl Carbamate Ionic Liquid and Ultrasound-Assisted Intensification

ACS Sustainable Chemistry & Engineering, Journal Year: 2025, Volume and Issue: 13(1), P. 68 - 79

Published: Jan. 3, 2025

Enzymatic biodiesel production often faces challenges such as mass transfer limitations and enzyme sensitivity, resulting in low reaction efficiency. This study aimed to address these issues by integrating ultrasound technology with low-cost CO2-based alkyl carbamate ionic liquids (ILs) additives. Among the investigated ILs, N,N-diallylammonium N′,N′-diallylcarbamate (DACARB) demonstrated highest fatty acid methyl ester (FAME) content ultrasound-assisted catalyzed Eversa Transform 2.0 at an optimal temperature of 50 °C. The addition DACARB improved solubility immiscible reactants enhanced lipase stability, maintaining high FAME yields even under amplitudes up 100% duty cycles 20%. Enzyme hydrolytic activity assays revealed that activated lipase, increasing 33% through favorable alterations tertiary structure, confirmed fluorescence spectroscopy. Furthermore, employing a stepwise methanol dosing strategy 40% amplitude 5% cycle presence 2 wt % achieved 91.3 within 12 h, using only 0.2 concentration. highlights potential promising additive enhance efficiency enzymatic production, offering solution overcome current process.

Language: Английский

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Effects of ultrasound treatment on the structure, function properties and in vitro digestion of Sipunculus nudus protein

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134422 - 134422

Published: Aug. 8, 2024

Language: Английский

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Fish gut symbiotic bacterium Bacillus thuringiensis: RSM optimization for its extracellular lipase enzyme production, lipase-protein purification, characterization, and docking analysis

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140428 - 140428

Published: Jan. 1, 2025

Language: Английский

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Exploring combi-lipase catalysis of Eversa® Transform and Candida rugosa lipases for enzymatic hydrolysis of crude palm oil: Effects on fatty acid yield and retention of carotene and tocols

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141650 - 141650

Published: March 1, 2025

Several lipases have been used to catalyze the hydrolysis of crude palm oil (CPO) concentrate phytonutrients. Previously, retention phytonutrients during has improved with a small addition ethylenediaminetetraacetic acid. However, production free fatty acids (FFA) via enzymatic yet be optimised. Thus, for first time, this study explored combined use AY 30SD (from Candida rugosa) and Eversa® Transform 2.0, ET 2.0 Aspergillus oryzae) investigate effect reaction time temperature on lipases' performance towards FFA alcohol-free process producing concentrates. The results revealed that application combi-lipases at 45 °C yielded highest final content due 2.0's affinity longer acid chain lengths 30SD's effectiveness in hydrolysing TAG molecules. With total enzyme concentration 0.2 wt%, incorporating combi-lipase produced >95 wt% FFA. Furthermore, basic economic analysis validated combination 0.15 0.05 reduced by seven times (completed within 24 h), potentially leading substantial increase profit without sacrificing retention.

Language: Английский

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Sustainable Alternative Media for the Production of Lipolytic Cells and Fatty Acid Concentrates: Integration of the Enzyme and Food Industries

Foods, Journal Year: 2025, Volume and Issue: 14(6), P. 990 - 990

Published: March 14, 2025

The use of agro-industrial by-products and processing residues, which are rich in carbohydrates, proteins, lipids, the production lipases allows sustainable these reducing environmental impacts. In this study, immersion water lentils, soybeans, textured soy protein was evaluated as carbon nitrogen sources whole-cell lipases, resulting biomass used hydrolysis residual soybean oil with conventional heating ultrasound. results showed that best culture medium one 50% protein, reaching values 149.04 U/g hydrolytic activity, 12.92 g/L concentration, 144.17 U total specific volumetric productivities 2.07 U/g·h 20.02 U/L·h, respectively. positive effect adding frying to crop observed, increased cell activity. obtained potential for ultrasound-assisted vegetable oils, approximately 43.36% 7 h reaction, an initial rate 31.03 mmol/h. It is concluded a viable candidate replace traditional sources, being economically attractive alternative due its wide generation restaurants.

Language: Английский

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Comparative studies on enzymatic changes and structure of lipases under ultrasound

3 Biotech, Journal Year: 2025, Volume and Issue: 15(6)

Published: May 2, 2025

Language: Английский

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Structural, functional and antioxidant characteristics of enzymatic hydrolysates from Cantharellus cibarius protein

Food Chemistry, Journal Year: 2025, Volume and Issue: 486, P. 144626 - 144626

Published: May 8, 2025

Language: Английский

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Unveiling the role of mechanical process intensifications and chemical additives in boosting lipase-catalyzed hydrolysis of vegetable oil for fatty acid production: A comprehensive review

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138144 - 138144

Published: Nov. 1, 2024

Language: Английский

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Ultrases İşleminin Enzim Aktivasyonu Üzerine Etkileri

Akademik Gıda, Journal Year: 2024, Volume and Issue: 22(2), P. 158 - 166

Published: Sept. 4, 2024

Ultrases (ultrason) işlemi, özellikle gıda, biyoteknoloji ve biyofarmasötik endüstrileri olmak üzere çeşitli alanlarda kullanılan yeşil etkili bir termal olmayan işlem tekniği olarak son yıllarda dikkat çekmektedir. Ultrason işleminin bu endüstrilerde önemli uygulamalara sahip enzimatik reaksiyonlarda etkilerinin belirlenmesi önemlidir. Yoğun ultrason koşulları enzim inaktivasyonuna neden olurken ılımlı koşullarında (yani düşük yoğunluk kısa süre) aktivitesinin arttırılabildiği bildirilmektedir. işlemi serbest enzime, substratta immobilize enzime uygulanabilir. Ultrasonikasyon substrat yapısı ile hidroliz kinetiği termodinamik parametreleri üzerine etkileri bulunmaktadır. Bu işlem, moleküllerin yapısını olumlu şekilde değiştirebilir böylece aktivitesi ürün verimi arttırılabilir. derlemede enzim, substrata uygulanması sonucu meydana gelen moleküller yapıdaki aktivasyonundaki değişiklikler, değişikliklerin mekanizmaları etkileyen faktörler ilgili bilgiler verilmiştir.

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