The diagnostic value of a breast cancer diagnosis model based on serum MiRNAs and serum tumor markers

World Journal of Surgical Oncology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 29, 2025

Abstract Background Breast cancer (BCa) is the leading cause of cancer-related death among women worldwide. MicroRNAs (miRNAs) are promising tools for diagnosis and prognosis. This study investigated role serum miRNAs tumor markers (TMs) in BCa. Methods Differentially expressed were screened from samples BCa patients healthy individuals via high-throughput sequencing. The expression hsa-miR-1911-3p, hsa-miR-4694-5p, hsa-miR-548ao-5p, hsa-miR-4804-3p 169 116 controls was detected qRT-PCR. Serum tumor-associated antigens by chemiluminescence. Logistic regression subsequently used to develop miRNA panel I, TM II, (miRNA + TM) III models. Receiver operating characteristic (ROC) curve, precision-recall (PR) curve decision analyses (DCA) performed assess accuracy three models diagnosis. Additionally, relationships between clinical characteristics with assessed. Results Four (hsa-miR-1911-3p, hsa-miR-4804-3p) newly associated I based on hsa-miR-548ao-5p showed greater diagnostic effectiveness than II antigen 125 (CA125) 153 (CA153), AUC values 0.816 0.777, respectively. had higher an value 0.870. miR-548ao-5p miR-4804-3p closely related features, such as human epidermal growth factor receptor 2 (HER2), estrogen (ER), progesterone (PR), HER2-enriched subtype, stage III/IV, lymph node-transplanted breast cancer. Conclusion MiR-548ao-5p miR-4804-3 could serve potential biomarkers

Language: Английский

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Advances in understanding the role of lncRNA in ferroptosis

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17933 - e17933

Published: Aug. 26, 2024

LncRNA is a type of transcript with length exceeding 200 nucleotides, which was once considered junk no biological function during the transcription process. In recent years, lncRNA has been shown to act as an important regulatory factor at multiple levels gene expression, affecting various programmed cell death modes including ferroptosis. Ferroptosis, new form death, characterized by deficiency cysteine or inactivation glutathione peroxidase, leading depletion glutathione, aggregation iron ions, and lipid peroxidation. These processes are influenced many physiological processes, such Nrf2 pathway, autophagy, p53 pathway so on. An increasing number studies have that can block expression specific molecules through decoy effect, guide proteins function, promote interactions between scaffolds. action regulate key factors in metabolism, antioxidant metabolism epigenetic genetic regulation, thereby regulating process this review, we snapshotted mechanism ferroptosis example, emphasizing regulation on these pathways, helping fully understand evolution fate.

Language: Английский

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LINC01614 Promotes Oral Squamous Cell Carcinoma by Regulating FOXC1

Genes, Journal Year: 2024, Volume and Issue: 15(11), P. 1461 - 1461

Published: Nov. 13, 2024

Background: Long non-coding RNAs (lncRNAs) are pivotal mediators during the development of carcinomas; however, it remains to be investigated whether lncRNAs implicated in oral squamous cell carcinoma (OSCC). Methods: In this study, quantitative real-time PCR was conducted for detecting expression LINC01614 OSCC lines. The biological functions were assessed by loss- and gain-of-function experiments both vivo vitro. Cellular proliferation, migration, invasion herein, dual luciferase reporter assays additionally performed explore relationships among LINC01614, miR-138-5p, Forkhead box C1 (FOXC1). Results: research presented herein revealed that cells express high levels LINC01614. Functional employing cellular animal models demonstrated knockdown repressed malignant phenotypes cells, including their growth, invasiveness, migration. Further investigation absorbs miR-138-5p miRNA functioning as a competing endogenous RNA downregulate abundance FOXC1. Conclusions: findings contributes progression targeting FOXC1 signaling pathway. study provides insights into novel mechanistic process regulate OSCC, established possible target therapeutic management OSCC.

Language: Английский

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Unraveling non-coding RNAs in breast cancer: mechanistic insights and therapeutic potential

Medical Oncology, Journal Year: 2024, Volume and Issue: 42(1)

Published: Dec. 27, 2024

Language: Английский

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