Postmortem Changes of Desmin, Talin-2, Vinculin, and Integrin β1 in Chicken Breast and Their Effects on Tenderness DOI

Wanhong He,

Yingbo Peng,

Haixia Huang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Costameres are essential for maintaining the integrity of muscle fibers, which affects meat tenderness. To explore pattern alteration in costameres after slaughter, this study investigated distribution costamere proteins (desmin, talin-2, vinculin, and integrin β1), their impact on tenderness, involved enzymes. Western blot analysis showed that talin-2 significantly degraded postmortem, while β1 increased at 48 h (P < 0.05). Warner-Bratzler shear force was positively correlated with desmin, expression transcription levels genes but negatively pH, expression, ITGB1 level terms overall trend. Multiplex immunohistochemistry revealed these were mainly distributed sarcolemma cytoplasm, spatial interactions them began to weaken 24 h. Caspase-3 cathepsin B colocalized 3 may contribute degradation costameres.

Language: Английский

Postmortem Changes of Desmin, Talin-2, Vinculin, and Integrin β1 in Chicken Breast and Their Effects on Tenderness DOI

Wanhong He,

Yingbo Peng,

Haixia Huang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Costameres are essential for maintaining the integrity of muscle fibers, which affects meat tenderness. To explore pattern alteration in costameres after slaughter, this study investigated distribution costamere proteins (desmin, talin-2, vinculin, and integrin β1), their impact on tenderness, involved enzymes. Western blot analysis showed that talin-2 significantly degraded postmortem, while β1 increased at 48 h (P < 0.05). Warner-Bratzler shear force was positively correlated with desmin, expression transcription levels genes but negatively pH, expression, ITGB1 level terms overall trend. Multiplex immunohistochemistry revealed these were mainly distributed sarcolemma cytoplasm, spatial interactions them began to weaken 24 h. Caspase-3 cathepsin B colocalized 3 may contribute degradation costameres.

Language: Английский

Citations

0