Patterns of Isoform Variation for N Gene Subgenomic mRNAs in Betacoronavirus Transcriptomes DOI Creative Commons

James J. Kelley,

Andrey Grigoriev

Viruses, Journal Year: 2024, Volume and Issue: 17(1), P. 36 - 36

Published: Dec. 30, 2024

The nucleocapsid (N) protein is the most expressed in later stages of SARS-CoV-2 infection with several important functions. It translated from a subgenomic mRNA (sgmRNA) formed by template switching during transcription. A recently described translation initiation site (TIS) CTG codon leader sequence (TIS-L) out frame structural and accessory genes including N gene may act as suppressor. We analyzed multiple sequenced samples infected found that any single variant this virus produces isoforms sgmRNA. main isoform starting at TIS-L frame, but two secondary dominant (present nearly all samples) were to restore reading likely involved regulation production. Analysis other coronaviruses revealed such are also produced their transcriptomes. In SARS-CoV, they for putative TIS (also codon) same relative position SARS-CoV-2. Positions junction breakpoints stem loop 3 5'-UTR suggest similar mechanisms SARS-CoV-2, OC43, not MERS-CoV. These observations be pertinent antisense-based antiviral strategies.

Language: Английский

Optimizing Clinical Management of COVID-19: A Predictive Model for Unvaccinated Patients Admitted to ICU DOI Creative Commons

Ahmed Farhan,

Khouloud Ayed,

Sara Zayati

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(3), P. 230 - 230

Published: Feb. 27, 2025

This study investigates the impact of immunological and clinical factors on COVID-19 outcomes among unvaccinated individuals. A cohort 42 patients admitted to an intensive care unit was analyzed, focusing age, comorbidities, inflammatory cytokines (IL-6, TNF-α), anti-SARS-CoV-2 spike protein antibody levels (IgG) assess their influence hospital stay duration, recovery time, complications, mortality rates. The findings revealed that advanced cardiovascular disease, elevated pro-inflammatory significantly heightened risks severe complications mortality. Conversely, low IgG correlated with prolonged stays slower recovery. Multivariate analysis identified high IL-6 TNF-α as strong predictors adverse outcomes. research emphasizes need for early monitoring targeted management strategies mitigate COVID-19, especially high-risk populations.

Language: Английский

Citations

0
Mycophenolate mofetil exerts broad-spectrum antiviral activity against coronaviruses including SARS-CoV-2 DOI Creative Commons
Mengyuan Wu, Kun Wang, Huiqiang Wang

et al.

Virology Journal, Journal Year: 2025, Volume and Issue: 22(1)

Published: March 4, 2025

Language: Английский

Citations

0
The Clinical and Laboratory Landscape of COVID-19 During the Initial Period of the Pandemic and at the Beginning of the Omicron Era DOI Creative Commons
Yulia Desheva, Tamara Shvedova, Olga Kopteva

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 481 - 481

Published: March 27, 2025

Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) underwent significant mutations, resulting in the Omicron variant. Methods: In this study, we analyzed blood samples from 98 patients with disease 19 (COVID-19) hospitalized during initial SARS-CoV-2 wave and onset of 2021. High-resolution melting (HRM) analysis PCR products was used to analyze RNA extracted clinical collected July November 2021 infected SARS-CoV-2. Results: HRM revealed a characteristic deletion N protein virus isolated 2021, associated Elevated levels inflammatory markers interleukin-6 (IL-6) were observed both waves COVID-19. Complement IgG IgM antibodies detected more often second wave. An increase hemagglutinin-inhibiting (HI) against influenza viruses paired specimens moderate severe COVID-19 outbreaks. Conclusions: Patients admitted showed rise markers, suggesting that triggers responses. The rapid formation may indicate faster immune response. Seasonal flu negatively impact course infections.

Language: Английский

Citations

0
Virus-like particles: Innovative strategies for combatting emerging and re-emerging viral threats DOI Creative Commons

Bugude Laxmi,

P. Uma Maheswari Devi,

Naveen Thanjavur

et al.

The Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 100351 - 100351

Published: April 1, 2025

Language: Английский

Citations

0
SARS-CoV-2 Infection and Alpha-Synucleinopathies: Potential Links and Underlying Mechanisms DOI Open Access
Joanna Motyl, Grażyna Gromadzka, Grzegorz A. Czapski

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12079 - 12079

Published: Nov. 10, 2024

Alpha-synuclein (α-syn) is a 140-amino-acid, intrinsically disordered, soluble protein that abundantly present in the brain. It plays crucial role maintaining cellular structures and organelle functions, particularly supporting synaptic plasticity regulating neurotransmitter turnover. However, for reasons not yet fully understood, α-syn can lose its physiological begin to aggregate. This altered disrupts dopaminergic transmission causes both presynaptic postsynaptic dysfunction, ultimately leading cell death. A group of neurodegenerative diseases known as α-synucleinopathies characterized by intracellular accumulation deposits specific neuronal glial cells within certain brain regions. In addition Parkinson's disease (PD), these conditions include dementia with Lewy bodies (DLBs), multiple system atrophy (MSA), pure autonomic failure (PAF), REM sleep behavior disorder (RBD). Given disorders are associated α-syn-related neuroinflammation-and considering SARS-CoV-2 infection has been shown affect nervous system, COVID-19 patients experiencing neurological symptoms-it proposed may contribute neurodegeneration PD other promoting misfolding aggregation. this review, we focus on whether could act an environmental trigger facilitates onset or progression α-synucleinopathies. Specifically, new evidence potential modulating function discuss causal relationship between development parkinsonism-like symptoms.

Language: Английский

Citations

1
Patterns of Isoform Variation for N Gene Subgenomic mRNAs in Betacoronavirus Transcriptomes DOI Creative Commons

James J. Kelley,

Andrey Grigoriev

Viruses, Journal Year: 2024, Volume and Issue: 17(1), P. 36 - 36

Published: Dec. 30, 2024

The nucleocapsid (N) protein is the most expressed in later stages of SARS-CoV-2 infection with several important functions. It translated from a subgenomic mRNA (sgmRNA) formed by template switching during transcription. A recently described translation initiation site (TIS) CTG codon leader sequence (TIS-L) out frame structural and accessory genes including N gene may act as suppressor. We analyzed multiple sequenced samples infected found that any single variant this virus produces isoforms sgmRNA. main isoform starting at TIS-L frame, but two secondary dominant (present nearly all samples) were to restore reading likely involved regulation production. Analysis other coronaviruses revealed such are also produced their transcriptomes. In SARS-CoV, they for putative TIS (also codon) same relative position SARS-CoV-2. Positions junction breakpoints stem loop 3 5'-UTR suggest similar mechanisms SARS-CoV-2, OC43, not MERS-CoV. These observations be pertinent antisense-based antiviral strategies.

Language: Английский

Citations

0