Optimizing Fermentation of Morus nigra L. Residues with Schizophyllum commune to Enhance Anthocyanin Release and Anti-Inflammatory Activity via Pyroptosis Pathway Modulation DOI Creative Commons

Lin Ye,

Qin Hu, Ying Lin

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(3), P. 145 - 145

Published: March 14, 2025

Morus nigra L. is rich in anthocyanins and other active ingredients, but its extraction residues pose a burden on the environment. In present study, residue resource utilization was achieved through liquid fermentation of Schizophyllum commune, with aim enhancing anthocyanin solubilization evaluating anti-inflammatory activity. Response surface methodology used to optimize parameters quantify fractions by HPLC. The effect evaluated using lipopolysaccharide-induced inflammation model human foreskin fibroblast (BJ cell), interaction cyanidin-3-O-glucoside (C3G) NLRP3, key target pyroptosis pathway, resolved molecular docking. Our results indicated that optimal conditions (substrate 3.4%, inoculum 9%, time 50 h) enabled total reach 85.1 μg/mL, which C3G content elevated 66.7 μg/mL (release efficiency 83.9%). fermented filtrate effectively promoted BJ cell proliferation inhibited inflammatory response, signaling pathway playing significant role. Molecular docking confirmed binds strongly NLRP3 protein. This technology provides new strategy for high-value development natural drugs.

Language: Английский

Editing a mushroom with high-digestibility using a novel endo-N-acetyl-β-D-glucosaminidase DOI
Ying Wang,

Wenjing Chen,

Hongyu Chen

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 305, P. 141165 - 141165

Published: Feb. 17, 2025

Language: Английский

Citations

0
Optimizing Fermentation of Morus nigra L. Residues with Schizophyllum commune to Enhance Anthocyanin Release and Anti-Inflammatory Activity via Pyroptosis Pathway Modulation DOI Creative Commons

Lin Ye,

Qin Hu, Ying Lin

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(3), P. 145 - 145

Published: March 14, 2025

Morus nigra L. is rich in anthocyanins and other active ingredients, but its extraction residues pose a burden on the environment. In present study, residue resource utilization was achieved through liquid fermentation of Schizophyllum commune, with aim enhancing anthocyanin solubilization evaluating anti-inflammatory activity. Response surface methodology used to optimize parameters quantify fractions by HPLC. The effect evaluated using lipopolysaccharide-induced inflammation model human foreskin fibroblast (BJ cell), interaction cyanidin-3-O-glucoside (C3G) NLRP3, key target pyroptosis pathway, resolved molecular docking. Our results indicated that optimal conditions (substrate 3.4%, inoculum 9%, time 50 h) enabled total reach 85.1 μg/mL, which C3G content elevated 66.7 μg/mL (release efficiency 83.9%). fermented filtrate effectively promoted BJ cell proliferation inhibited inflammatory response, signaling pathway playing significant role. Molecular docking confirmed binds strongly NLRP3 protein. This technology provides new strategy for high-value development natural drugs.

Language: Английский

Citations

0