Reversed‐Phase High‐Performance Liquid Chromatography Tandem Mass Spectrometry Method for Quantification of Zanamivir in Rat Plasma and the Application in a Pharmacokinetics Study DOI

Huang Ziqian,

Wan Lili,

Chen Houyi

et al.

Separation Science Plus, Journal Year: 2025, Volume and Issue: 8(4)

Published: April 1, 2025

ABSTRACT A RP‐HPLC‐MS/MS method has been developed for the determination of zanamivir in rat plasma present study first time. The analytes were retained and separated on a C18 column triple‐quadrupole mass spectrometry equipped with an electrospray ionization (ESI) source was applied quantification. Zanamivir could be well under appropriate chromatographic conditions. solid phase extraction used to pretreat plasma. recovery above 83% matrix effect negligible. linear over concentration ranges 1.6–3200.0 ng/mL zanamivir. precision accuracy within acceptable limits. lowest limit quantification (LLOQ) 1.6 ng/mL. on‐column sensitivity higher than reported sensitivity. This simple validated successfully support pharmacokinetics after administered Sprague‐Dawley rats.

Language: Английский

A novel tetravalent influenza vaccine based on one chimpanzee adenoviral vector DOI

Yixin Niu,

Yao Yan,

Ying Hu

et al.

Vaccine, Journal Year: 2025, Volume and Issue: 53, P. 126959 - 126959

Published: March 1, 2025

Language: Английский

Citations

0
Reversed‐Phase High‐Performance Liquid Chromatography Tandem Mass Spectrometry Method for Quantification of Zanamivir in Rat Plasma and the Application in a Pharmacokinetics Study DOI

Huang Ziqian,

Wan Lili,

Chen Houyi

et al.

Separation Science Plus, Journal Year: 2025, Volume and Issue: 8(4)

Published: April 1, 2025

ABSTRACT A RP‐HPLC‐MS/MS method has been developed for the determination of zanamivir in rat plasma present study first time. The analytes were retained and separated on a C18 column triple‐quadrupole mass spectrometry equipped with an electrospray ionization (ESI) source was applied quantification. Zanamivir could be well under appropriate chromatographic conditions. solid phase extraction used to pretreat plasma. recovery above 83% matrix effect negligible. linear over concentration ranges 1.6–3200.0 ng/mL zanamivir. precision accuracy within acceptable limits. lowest limit quantification (LLOQ) 1.6 ng/mL. on‐column sensitivity higher than reported sensitivity. This simple validated successfully support pharmacokinetics after administered Sprague‐Dawley rats.

Language: Английский

Citations

0