SlUPA-like, a bHLH Transcription Factor in Tomato (Solanum lycopersicum), Serves as the Crosstalk of GA, JA and BR DOI Open Access
Pengyu Guo, Xin Cheng, Yunshu Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13419 - 13419

Published: Dec. 14, 2024

The bHLH (basic Helix–Loop–Helix) transcription factor serves as pivotal controller in plant growth and development. In a previous study, the overexpression of SlUPA-like Solanum lycopersicum L. Ailsa Craig (AC++) altered JA (Jasmonic acid) response endogenous GA (Gibberellic content. However, detailed regulation mechanism was not fully explored. present research, we found that influenced accumulation GA, BR (Brassinolide). RNA-Seq data illustrated expression levels genes related to these hormones were significantly affected. Additionally, interaction with SlMYB21, SlMYC2 SlDELLA characterized by employing Y2H (Yeast Two-Hybrid) BiFC (Bimolecular Fluorescence Complementation) assay. Furthermore, Dual-LUC (Dual-Luciferase) assay EMSA (Electrophoretic Mobility Shift Assay) identified directly targeted E-box motif promoter SlGID2 activated SlGID2. These results shed light on potential role mediating crosstalk among multiple established robust theoretical framework for further unraveling functions factors context hormone signal transduction.

Language: Английский

Enhancing Turfgrass Growth Under Low-Light Conditions: The Novel Application of Long Afterglow Luminescent Materials and Glass Microsphere Technology DOI
Yilin Wang, Mingming Shi, Bobo Yang

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Genome-Wide Identification of GATA Transcription Factors in Acorus and Expression Patterns Under Abiotic Stress DOI Creative Commons
Liang Ma, Cheng‐Yuan Zhou,

C. S. Gong

et al.

Horticulturae, Journal Year: 2025, Volume and Issue: 11(4), P. 410 - 410

Published: April 11, 2025

The GATA transcription factors are a class of DNA-binding proteins that control the expression target genes by binding to promoter regions DNA, thereby affecting growth and development plants. In recent years, have been discovered functionally characterized in many plant species. However, there is limited annotation information for genus Acorus. This study identified 38 20 Acorus calamus A. gramineus, respectively. Based on phylogenetic tree, they were classified into four subfamilies (I, II, III, IV). At same time, an analysis domain motifs revealed domains relatively conserved across all subfamilies. A multiple sequence alignment showed almost highly at LCNACG site. An cis-elements, collinearity, protein–protein interaction networks provided insights response mechanisms under biotic stress. Additionally, we found significant changes gramineus drought salt These results provide useful future functional analyses contribute better understanding stress molecular breeding directions

Language: Английский

Citations

0
SlUPA-like, a bHLH Transcription Factor in Tomato (Solanum lycopersicum), Serves as the Crosstalk of GA, JA and BR DOI Open Access
Pengyu Guo, Xin Cheng, Yunshu Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13419 - 13419

Published: Dec. 14, 2024

The bHLH (basic Helix–Loop–Helix) transcription factor serves as pivotal controller in plant growth and development. In a previous study, the overexpression of SlUPA-like Solanum lycopersicum L. Ailsa Craig (AC++) altered JA (Jasmonic acid) response endogenous GA (Gibberellic content. However, detailed regulation mechanism was not fully explored. present research, we found that influenced accumulation GA, BR (Brassinolide). RNA-Seq data illustrated expression levels genes related to these hormones were significantly affected. Additionally, interaction with SlMYB21, SlMYC2 SlDELLA characterized by employing Y2H (Yeast Two-Hybrid) BiFC (Bimolecular Fluorescence Complementation) assay. Furthermore, Dual-LUC (Dual-Luciferase) assay EMSA (Electrophoretic Mobility Shift Assay) identified directly targeted E-box motif promoter SlGID2 activated SlGID2. These results shed light on potential role mediating crosstalk among multiple established robust theoretical framework for further unraveling functions factors context hormone signal transduction.

Language: Английский

Citations

0