UGT73FB1 contributes to scaffold‐selective biosynthesis of triterpenoid glucosyl esters in saponin‐rich bark of arjuna tree DOI

Poonam Vyas,

Payal Srivastava,

Gaurav Srivastava

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(1)

Published: April 1, 2025

SUMMARY Plants make structurally diverse triterpenoids for their physiological needs, which have shown numerous therapeutic applications. Arjuna tree ( Terminalia arjuna ) produces bioactive oleanane (β‐amyrin‐derived) arjunic acid, arjungenin, and arjunolic the respective C28‐ O ‐glucopyranosyl esters arjunetin, arjunglucoside I, II. Arjunic acid arjunetin are major oleananes in bark, while II found minor levels. Although arjungenin was detected at a considerable level, I only trace suggesting selective biosynthesis and/or accumulation of triterpenoid glucosyl bark. However, enzyme contributing to ‐glucosylation not characterized. We mined RNA‐sequencing data identified UDP‐glucosyltransferase (UGT) transcripts that were enriched bark transcriptome. Further, biochemical screening UGTs UGT73FB1, catalyzed scaffold‐selective manner. Recombinant UGT73FB1 produced Escherichia coli or Nicotiana benthamiana formed II, but ester (arjunglucoside I). Interestingly, showed better activity using than ursanes (α‐amyrin‐derived), it did show various lupane (lupeol‐derived). Overall, spatial patterns transcript expression suggested role arjuna. Moreover, co‐expression with β‐amyrin synthase C2, C23, C28 hydroxylases/oxidases led complete reconstruction pathway N. , utility enzymes rare glucopyranosyl heterologous hosts.

Language: Английский

Identification and characteristic analysis of PavUGT48 as a novel UDP-glycosyltransferase with dual functions on anthocyanin and amygdalin biosynthesis in sweet cherry DOI
Feng Chen,

Qingqing Guo,

Chuanbao Wu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143062 - 143062

Published: April 1, 2025

Language: Английский

Citations

0
UGT73FB1 contributes to scaffold‐selective biosynthesis of triterpenoid glucosyl esters in saponin‐rich bark of arjuna tree DOI

Poonam Vyas,

Payal Srivastava,

Gaurav Srivastava

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(1)

Published: April 1, 2025

SUMMARY Plants make structurally diverse triterpenoids for their physiological needs, which have shown numerous therapeutic applications. Arjuna tree ( Terminalia arjuna ) produces bioactive oleanane (β‐amyrin‐derived) arjunic acid, arjungenin, and arjunolic the respective C28‐ O ‐glucopyranosyl esters arjunetin, arjunglucoside I, II. Arjunic acid arjunetin are major oleananes in bark, while II found minor levels. Although arjungenin was detected at a considerable level, I only trace suggesting selective biosynthesis and/or accumulation of triterpenoid glucosyl bark. However, enzyme contributing to ‐glucosylation not characterized. We mined RNA‐sequencing data identified UDP‐glucosyltransferase (UGT) transcripts that were enriched bark transcriptome. Further, biochemical screening UGTs UGT73FB1, catalyzed scaffold‐selective manner. Recombinant UGT73FB1 produced Escherichia coli or Nicotiana benthamiana formed II, but ester (arjunglucoside I). Interestingly, showed better activity using than ursanes (α‐amyrin‐derived), it did show various lupane (lupeol‐derived). Overall, spatial patterns transcript expression suggested role arjuna. Moreover, co‐expression with β‐amyrin synthase C2, C23, C28 hydroxylases/oxidases led complete reconstruction pathway N. , utility enzymes rare glucopyranosyl heterologous hosts.

Language: Английский

Citations

0