Aquaculture International, Journal Year: 2025, Volume and Issue: 33(4)
Published: April 23, 2025
Language: Английский
Aquaculture International, Journal Year: 2025, Volume and Issue: 33(4)
Published: April 23, 2025
Language: Английский
Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 25, 2025
Sitophilus zeamais is a highly destructive pest of stored grains worldwide. To develop novel RNA insecticides, the double-stranded S. cytochrome c oxidase (dsSzCOXs: dsSzCOXI, dsSzCOXII, and dsSzCOXIII) was successfully expressed in pET28a-BL21(DE3)-RNaseIII- system remarkably downregulated expression level SzCOXs through injection or feeding methods, leading to high mortalities both larvae adults. Under their optimal conditions (isopropyl β-d-thiogalactoside, 0.6 mM), yields dsSzCOXI (at 28 °C for 4 h), dsSzCOXII 37 6 dsSzCOXIII h) were 8.49, 5.80, 6.04 μg/mL respectively. The chitosan-based gene delivery composed dsSzCOXs (CS@dsSzCOXs) established insecticidal effect adults via mixing wheat seeds, with mortality reaching 50% on day ten. Consequently, this nanoparticle-mediated expected be an effective approach control.
Language: Английский
Citations
0Aquaculture International, Journal Year: 2025, Volume and Issue: 33(4)
Published: April 23, 2025
Language: Английский
Citations
0