Effect of sheep placenta extract on D-galactose-induced aging mouse

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 26, 2025

Introduction Sheep placenta extract (SPE) is a representative traditional medicinal substance that exhibits multiple experimentally validated physiological properties, including anti-aging effects, wound healing acceleration, antioxidant activity, and anti-inflammatory mechanisms. However, the mechanism by which SPE influences delay of aging still not yet clear. Methods Exploring effects sheep on D-gal induced senescence in mouse model macrogenomics metabolomics. Results In serum mice treated with SPE, levels function such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) were notably higher compared to those blank group, whereas malondialdehyde (MDA) decreased. We revealed alleviated changes gut microbiota caused mice, significant decrease Firmicutes/Bacteroidetes (F/B) ratio gut. Furthermore, Akkermansia muciniphila ( A. ), known for its regulating immune response potential showed increase 1177.94%. The analysis UHPLC-QE-MS combined orthogonal partial least squares discriminant (OPLS-DA) screening differential metabolites metabolic profiles upregulation cis -5,8,11,14,17-eicosapentaenoic acid (EPA) triptolide metabolites, following treatment, are commonly believed have immunosuppressive, anti-inflammatory, anti-proliferative, anti-tumor effects. Discussion role ameliorating may be associated increased abundance accumulation two EPA triptolide, serum.

Language: Английский

Integrated metabolomics and intestinal microbiota analysis to reveal anti-post-weaning diarrhea mechanisms of Modified Yupingfeng Granule in Rex rabbits

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 10, 2025

Post-weaning Diarrhea (PWD) is a kind of physiological stress diarrhea in Rex rabbits after weaning, which can lead to death severe cases. Traditional Chinese medicine (TCM) has been widely used animal due its advantages natural origin, diverse functions, safety, reliability, economy and environmental protection. Modified Yupingfeng Granule (MYPFG) an improved prescription based on the famous traditional (YPF), combined with other TCM obvious synergistic additive activity order obtain excellent for PWD. In this study, 120 weaned were randomly allocated 4 treatment groups, including control (CON), low dose (LD), medium (MD), high (HD). Rabbits fed diet or different MYPFG proportions 30 days. The study 16S rRNA analysis intestinal microbiota cecal contents metabolomics explore effect rabbits. increased average daily gain, villus length crypt depth ratio decreased feed meat ratio, frequency, mortality rate, (p < 0.05). test found that could change abundances Patescibacteria, Sphingobium, Ruminococcus, Oxalobacter. Metabolomics may be related regulation Glycine, serine threonine metabolism, Arginine proline metabolism. Nicotinate nicotinamide regulate metabolic pathway some amino acids alleviate PWD

Language: Английский

Effects of Dioscorea oppositifolia L. on growth performance, biochemical indicators, immunity, and intestinal health of weaned piglets

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: April 25, 2025

Introduction Weaning stress syndrome in piglets seriously endangers the healthy development of breeding industry. Dioscorea oppositifolia L. (Chinese yam, YAM) has activities such as boosting immunity and regulating gastrointestinal function. In order to explore potential efficacy YAM on weaned piglets, this study aimed investigate effects growth performance, immune function, intestinal health flora composition piglets. Methods Forty-eight 28-day-old were randomly divided into a control group, YAML group YAMH with 0, 1 2% added basal diet, respectively. During experiment, piglets’ feed intake was recorded, blood fecal samples collected. After feeding period, tissue colon content collected for testing. Results The results showed that adding diet can lower incidence diarrhea improve performance nutrient digestibility, reduce serum enzyme activity alanine aminotransferase (ALT), total cholesterol (TC) low-density lipoprotein (LDL-C); addition, also increase immunoglobulins (Ig) antibody titers, regulate level inflammatory factors, promote expression secretory immunoglobulin A (SIgA) protein intestine. Furthermore, supplementation villus height (VH), ratio crypt depth (V/C), Tight junctions (TJs), positive regulatory effect flora. Discussion summary, alleviates weaning by promoting improving function disease resistance, morphology mucosal immunity, microbial This provides theoretical basis application new plant-derived additive.

Language: Английский