Insight on the biomimetic of lysozyme interaction with functionalized iron oxide nanoparticles DOI
Wael M. Elshemey, Ahmed M. Elgharib, Abdo A. Elfiky

et al.

Therapeutic Delivery, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: Feb. 20, 2025

Lysozyme is a globular hydrolytic enzyme whose tissue level imperative for various clinical diagnostics. High levels of lysozyme are related to several inflammatory disorders, that breakdown cartilaginous tissues. Recently nanostructures have become widely used as modulators activity. This study delves into the influential role played by surface-modified iron oxide nanoparticles (IONPs) novel nano-inhibitors. Stern-Volmer plots results interaction with Cit-IONPs and Thy-IONPs reveal dynamic quenching constant (KSV) 40.075 65.714 ml/mg, binding (Kb) 1.539 × 103 4.418 free energy (∆G°binding) -43.563 KJ. mol-1 -49.821 mol-1, respectively. Upon IONPs, catalytic activity decreases due conjugation compared form enzyme. Computational approaches show citrate thymoquinone molecules affinities active residues about -4.3 -4.7 kcal/mol, Both formulations IONPs demonstrate high affinity toward proteins. work shows higher between than Cit-IONPs. These findings suggest represent promising class nano-inhibitors lysozyme, opening new avenues treating disorders associated overexpression.

Language: Английский

Insight on the biomimetic of lysozyme interaction with functionalized iron oxide nanoparticles DOI
Wael M. Elshemey, Ahmed M. Elgharib, Abdo A. Elfiky

et al.

Therapeutic Delivery, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: Feb. 20, 2025

Lysozyme is a globular hydrolytic enzyme whose tissue level imperative for various clinical diagnostics. High levels of lysozyme are related to several inflammatory disorders, that breakdown cartilaginous tissues. Recently nanostructures have become widely used as modulators activity. This study delves into the influential role played by surface-modified iron oxide nanoparticles (IONPs) novel nano-inhibitors. Stern-Volmer plots results interaction with Cit-IONPs and Thy-IONPs reveal dynamic quenching constant (KSV) 40.075 65.714 ml/mg, binding (Kb) 1.539 × 103 4.418 free energy (∆G°binding) -43.563 KJ. mol-1 -49.821 mol-1, respectively. Upon IONPs, catalytic activity decreases due conjugation compared form enzyme. Computational approaches show citrate thymoquinone molecules affinities active residues about -4.3 -4.7 kcal/mol, Both formulations IONPs demonstrate high affinity toward proteins. work shows higher between than Cit-IONPs. These findings suggest represent promising class nano-inhibitors lysozyme, opening new avenues treating disorders associated overexpression.

Language: Английский

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