Protective Effects of a Polyherbal Mixture on Intestinal Injury via the NF-κB Signaling Pathway and Gut Microbiota Modulation in Hyperuricemic Mice

Foods, Journal Year: 2025, Volume and Issue: 14(7), P. 1118 - 1118

Published: March 24, 2025

This study investigated the protective effects of a polyherbal tea (PHT) on intestinal injury in hyperuricemia (HUA) mice and underlying mechanisms. PHT was orally administered to for 49 days, while potassium oxonate hypoxanthine were 7 days after administration continued 42 cause HUA. Treatment with significantly reduced serum uric acid blood urea nitrogen levels HUA mice. It also inhibited liver xanthine oxidase activity promoted excretion through upregulation transporters GLUT9 ABCG2. Intestinal barrier integrity reinforced, as evidenced by restoration villous structure, reduction edema, tight junction proteins (occludin, ZO-1) mucin (MUC2). Moreover, suppressed LPS NF-κB pathway, leading TNF-α IL-6 gut. Gut microbiota analysis revealed reversed dysbiosis, enriching beneficial bacteria like Duncaniella sp. Heminiphilus faecis. By UPLC-MS analysis, 154 compounds persisted gut, suggesting that these are likely modulate both function gut microbiota. These findings suggest this may have potential functional food prevention hyperuricemia.

Language: Английский

Antioxidant and In Vitro Hepatoprotective Activities of a Polyphenol-Rich Fraction from the Peel of Citrus lumia Risso (Rutaceae)

Plants, Journal Year: 2025, Volume and Issue: 14(8), P. 1209 - 1209

Published: April 15, 2025

Citrus lumia Risso is an ancient, cultivated Mediterranean lime belonging to the Rutaceae family. It a species extremely difficult retrieve, but it still found in some private gardens certain regions of Southern Italy. fruits are rich source bioactive compounds, particularly polyphenols, which have been linked reduction risk several metabolic diseases. Here, hesperidium peel extracts were obtained by maceration with ethanol:water mixtures different proportions (50:50, 80:20, 0:100) and resulting crude then passed through glass column containing adsorbent resins concentrate polyphenolic compounds. After phytochemical characterization, evaluated for antioxidant activity using electron paramagnetic resonance (EPR) spectroscopy. Finally, water polyphenolic-rich extract (ClumWp), was highest flavonoid content (18.355 ± 1.607 mg/mL) strongest against hydroxyl radical, tested evaluate its potential protective effects on lipid accumulation both 2D hepatocyte cultures 3D spheroids. Treatment 25 50 μg/mL resulted intracellular HepG2 liver cell line, while treatment 100 µg/mL ClumWp + LX2 In addition, significantly increased ATP levels spheroids compared those untreated, suggesting ability restore promote production. Our results highlight that study neglected species, such as Risso, remains valuable opportunity valorize biodiversity, especially context applications improve human health. particular, fraction showed promising metabolism cellular energy balance may prove disorders MASLD, where disrupts normal functions.

Language: Английский

Urate-lowering effect of delphinidin-3-glucoside in red kidney beans via binding to the FAD site of the XO enzyme

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский