Comparison of different T cell assays for the retrospective determination of SARS-CoV-2 infection DOI
Benjamin A. Krishna, Marina Metaxaki, Marianne Perera

et al.

Journal of General Virology, Journal Year: 2024, Volume and Issue: 105(12)

Published: Dec. 20, 2024

It is important to be able retrospectively determine severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections with high accuracy, both for post-coronavirus disease 2019 (COVID-19) epidemiological studies, and distinguish between Long COVID other multi-syndromic diseases that have overlapping symptoms. Although serum antibody levels can measured diagnose SARS-CoV-2 infections, peptide stimulation of memory T cell responses a more sensitive approach. This because robust cells are generated after infection persist even antibodies wane below detectability thresholds. In this study, we compare using FluoroSpot-based methods overnight whole blood peptides followed by an ELISA. Both approaches comparable sensitivity specificity but require different equipment samples used. Furthermore, the elimination cross-react coronaviruses increases assay trades off some sensitivity. Finally, approach used on archival, cryopreserved PBMCs. work shows comparative advantages several measure could utilized any laboratory studying effects pandemic.

Language: Английский

Temporal Dynamics of Acute Coronary Syndromes During the COVID-19 Pandemic: A Retrospective Comparative Analysis Across Pre-Pandemic, Pandemic, and Post-Pandemic Phases DOI Creative Commons
Abdülkadir Çakmak, Ömer Kertmen

Acta Medica Nicomedia, Journal Year: 2025, Volume and Issue: 8(1), P. 45 - 51

Published: Feb. 28, 2025

Objective: The COVID-19 pandemic, caused by SARS-CoV-2, disrupted global healthcare systems and significantly influenced cardiovascular health. This study examines the temporal trends of acute coronary syndromes (ACS), including ST-elevation myocardial infarction (STEMI), non-ST-elevation (NSTEMI), unstable angina pectoris (USAP), across pre-pandemic, post-pandemic periods. Methods: retrospective observational was conducted at a single tertiary center involving patients diagnosed with ACS from January 2018 to June 2024. Patients were stratified into three cohorts: pre-pandemic (January 2018-February 2020), pandemic (March 2020-April 2022), (May 2022-June 2024). Results: incidence STEMI NSTEMI increased in period compared those other periods (p

Language: Английский

Citations

0

The 15‐Year Survival Advantage: Immune Resilience as a Salutogenic Force in Healthy Aging DOI Creative Commons
Muthu Saravanan Manoharan, Grace C. Lee, Nathan Harper

et al.

Aging Cell, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

ABSTRACT Human aging presents an evolutionary paradox: while rates remain constant, healthspan and lifespan vary widely. We address this conundrum via salutogenesis—the active production of health—through immune resilience (IR), the capacity to resist disease despite inflammation. Analyzing ~17,500 individuals across stages inflammatory challenges, we identified a core salutogenic mechanism: IR centered on TCF7 , conserved transcription factor maintaining T‐cell stemness regenerative potential. integrates innate adaptive immunity counter three mortality drivers: chronic inflammation (inflammaging), aging, cellular senescence. By mitigating these mechanisms, confers survival advantages: At age 40, with poor face 9.7‐fold higher rate—a risk equivalent that 55.5‐year‐olds optimal IR—resulting in 15.5‐year gap survival. Optimal preserves youthful profiles at any age, enhances vaccine responses, reduces burdens cardiovascular disease, Alzheimer's, serious infections. Two key themes emerge: first, female‐predominant IR, including likely reflects pressures favoring reproductive success caregiving; second, midlife (40–70 years) is critical window where by 69%. After 70, converge between resilient non‐resilient groups, reflecting biological limits longevity extension. TNFα‐blockers restore salutogenesis pathways, indicating delays aging‐related processes rather than altering rates. reframing as salutogenic‐pathogenic balance, establish ‐centered central healthy longevity. Targeted interventions enhance offer actionable strategies maximize before constraints limit benefits.

Language: Английский

Citations

0

Assessment of Post-COVID-19 Functional Status and Complications Among Survivors at a Tertiary Healthcare Center in Bangladesh DOI Open Access
Mohammad Tariqul Islam, Afsana Mahjabin, Md Mahbubul Islam

et al.

Cureus, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Language: Английский

Citations

0

Are Pre‐Hospitalization ECG Abnormalities Associated With Increased Mortality in COVID‐19 Patients? A Quantitative Systematic Literature Review DOI Creative Commons
Danielle Askey, Ann Smith

Annals of Noninvasive Electrocardiology, Journal Year: 2024, Volume and Issue: 29(6)

Published: Oct. 12, 2024

ABSTRACT Background While COVID‐19 is predominantly a respiratory disease, cardiovascular complications occur and are associated with worse outcomes. Electrocardiogram (ECG) abnormalities frequently observed in hospitalized patients, some of which increased mortality. It unclear whether ECG occurring before hospitalization This quantitative systematic literature review aims to determine changes mortality discuss these findings can aid the assessment patients decision‐making pre‐hospital environment. Methods A search following digital databases was conducted: CINAL, PUBMED, MEDLINE, Coronavirus Research Database. Eight cohort studies (primary papers) including ECGs taken Emergency Department were selected for synthesis results obtained prevalence among survivors compared non‐survivors. Odds hazard ratios also collected compared. Results Identification on pre‐hospitalization patients. These include non‐sinus rhythm, QTc prolongation, left bundle branch block, axis deviation, atrial fibrillation, flutter, right ventricular strain patterns, ST segment changes, T wave abnormalities, evidence hypertrophy. Conclusion environment may be beneficial when assessing could help identify at risk

Language: Английский

Citations

0

Comparison of different T cell assays for the retrospective determination of SARS-CoV-2 infection DOI
Benjamin A. Krishna, Marina Metaxaki, Marianne Perera

et al.

Journal of General Virology, Journal Year: 2024, Volume and Issue: 105(12)

Published: Dec. 20, 2024

It is important to be able retrospectively determine severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections with high accuracy, both for post-coronavirus disease 2019 (COVID-19) epidemiological studies, and distinguish between Long COVID other multi-syndromic diseases that have overlapping symptoms. Although serum antibody levels can measured diagnose SARS-CoV-2 infections, peptide stimulation of memory T cell responses a more sensitive approach. This because robust cells are generated after infection persist even antibodies wane below detectability thresholds. In this study, we compare using FluoroSpot-based methods overnight whole blood peptides followed by an ELISA. Both approaches comparable sensitivity specificity but require different equipment samples used. Furthermore, the elimination cross-react coronaviruses increases assay trades off some sensitivity. Finally, approach used on archival, cryopreserved PBMCs. work shows comparative advantages several measure could utilized any laboratory studying effects pandemic.

Language: Английский

Citations

0