Access Microbiology,
Journal Year:
2023,
Volume and Issue:
5(7)
Published: July 12, 2023
The
emergence
of
antibiotic
resistance
in
livestock,
especially
food-producing
animals,
is
major
public
health
importance
as
a
result
the
possibility
these
bacteria
entering
food
chain.
In
this
study,
genetic
characteristics
antibiotic-resistant
Escherichia
coli
and
Klebsiella
spp.
isolates
from
humans
poultry
Edo
state,
Nigeria,
were
investigated.
April
2017,
45
46
E.
obtained
urine,
clinical
wounds,
nasal
chicken
faecal
samples.
Isolates
recovered
identified
previously
described.
Species
identification
was
achieved
by
matrix-assisted
laser
desorption
ionization-time
flight
(MALDI-TOF)
mass
spectrometry
ribosomal
multilocus
sequence
typing.
Antimicrobial
susceptibility
testing
carried
out
using
Kirby-Bauer
method
for
12
antibiotics.
A
double
disc
synergy
test
used
to
screen
extended-spectrum
beta-lactamse
(ESBL)
production.
Whole
genome
sequencing
performed
strain
characterization
isolates.
Thirteen
yielded
positive
results
ESBL
phenotypic
harboured
genes.
Of
isolates,
21
human
13
resistant
at
least
one
tested
Four
genes
revealed
when
applying
tests.
include
blaCTX-M-15
blaSHV-28.
genome-based
core
gene
typing
close
relatedness
among
An
integrated
'One
Health'
surveillance
system
required
monitor
transmission
antimicrobial
Nigeria.
Antibiotics,
Journal Year:
2023,
Volume and Issue:
12(6), P. 1061 - 1061
Published: June 15, 2023
Escherichia
coli
are
one
of
the
most
important
pathogenic
bacteria
readily
found
in
livestock
and
widely
studied
as
an
indicator
that
carries
drug-resistant
genes
between
humans,
animals,
environment.
The
use
antimicrobials
food
chain,
particularly
food-producing
is
recognized
a
significant
contributor
to
development
spread
antimicrobial
resistance
(AMR)
can
be
transferred
from
farm
through
food-chain.
objective
this
review
highlight
background
more
specifically,
study
clonal
lineages
profiles
observed
E.
coli,
well
extended
spectrum
beta-lactamases
(ESBL)
producing
set
food-production
animals
with
greater
relevance
consumption,
such
pigs,
poultry,
cattle,
fish
farming
rabbits.
Regarding
prevalence
ESBL-producing
among
high-to-moderate
was
observed,
highest
rates
tetracycline
ampicillin
detected
different
farms
all
geographic
regions.
Worldwide
pandemic
clones
high-risk
zoonotic
have
been
identified
some
these
already
disseminated
niches,
environment
humans.
A
better
understanding
epidemiology
urgently
needed.
Animal
production
major
causes
antibiotic
problem
worldwide
One
Health
approach
Environment International,
Journal Year:
2024,
Volume and Issue:
191, P. 108972 - 108972
Published: Aug. 22, 2024
Overutilization
and
misuse
of
antibiotics
in
recent
decades
markedly
intensified
the
rapid
proliferation
diffusion
antibiotic
resistance
genes
(ARGs)
within
environment,
thereby
elevating
ARGs
to
status
a
global
public
health
crisis.
Recognizing
that
soil
acts
as
critical
reservoir
for
ARGs,
environmental
researchers
have
made
great
progress
exploring
sources,
distribution,
spread
soil.
However,
microscopic
state
micro-interfacial
behavior
remains
inadequately
understood.
In
this
study,
we
reviewed
behaviors
antibiotic-resistant
bacteria
(ARB)
porous
media,
predominantly
including
migration-deposition,
adsorption,
biofilm
formation.
Meanwhile,
proliferation,
degradation
were
identified
primary
soil,
with
component
serving
significant
determinant.
Our
work
contributes
further
comprehension
microstates
processes
ARB
environments
offers
theoretical
foundation
managing
mitigating
risks
associated
ARG
contamination.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Feb. 21, 2025
Antimicrobial
resistance
in
raw
milk
is
a
critical
challenge
for
public
health.
This
study
aimed
to
investigate
the
in-vitro
and
in-silico
analysis
of
antimicrobial
resistance,
mutations,
virulence
factors
(VFs),
mobile
genetic
elements,
diversity
Escherichia
coli
cattle
Gujarat,
India
during
May
2023-
April
2024.
56
isolates
were
recovered
from
50
samples.
Among
them,
26
identified
as
E.
subjected
susceptibility
testing.
The
extensively
drug-resistant
10
further
confirmed
by
16s
rRNA
identification.
Whole-genome
Nanopore
sequencing
was
performed
on
representative
strains,
data
used
bioinformatics
analysis.
results
revealed
that
wide
array
ARGs
mutations
detected.
Plasmid-mediated
detected
three
include
blaTEM-1,
sul2,
dfrA14,
aph6-Id,
aph(3'')-Ib,
tet(B),
responsible
against
antibiotic
classes
such
beta-lactam,
folate-pathway,
aminoglycosides,
tetracyclines,
respectively.
Additionally,
conserved
VFs
facilitated
serum
survival
toxin
production.
unit
transposons
Tn2
Tn10
two
isolates.
exhibited
considerable
diversity,
belonging
different
serotypes,
sequence
types
(ST),
phylogroups.
research
provides
crucial
insights
developing
effective
monitoring,
mitigation,
educational
strategies
prevent
spread
pathogenic
through
food
chain.
Environmental Research,
Journal Year:
2023,
Volume and Issue:
246, P. 118052 - 118052
Published: Dec. 30, 2023
The
Nordic
countries
(Denmark,
Finland,
Iceland,
Norway,
and
Sweden)
have
effectively
kept
lower
antibiotic-resistant
bacterial
(ARB)
pathogen
rates
than
many
other
countries.
However,
in
recent
years,
these
five
encountered
a
rise
ARB
cases
challenges
treating
infections
due
to
the
growing
prevalence
of
pathogens.
Wastewater-based
surveillance
(WBS)
is
valuable
supplement
clinical
methods
for
surveillance,
but
there
lack
comprehensive
understanding
WBS
application
This
review
aims
compile
latest
state-of-the-art
developments
monitoring
compare
them
with
practices.
After
reviewing
1480
papers
from
primary
search,
54
were
found
relevant,
15
additional
WBS-related
included.
Among
69
studies
analyzed,
42
dedicated
epidemiology,
while
27
focused
on
wastewater
monitoring.
PRISMA
literature
revealed
that
focus
four
major
objectives
ARB:
assessing
human
population,
identifying
evading
treatment,
quantifying
removal
rates,
evaluating
potential
evolution
during
treatment
process.
In
both
contexts,
most
studied
targets
pathogens
producing
carbapenemase
extended-spectrum
beta-lactamase
(ESBL),
primarily
Escherichia
coli
Klebsiella
spp.
vancomycin-resistant
Enterococcus
(VRE)
methicillin-resistant
Staphylococcus
aureus
(MRSA)
received
more
attention
epidemiology
studies,
probably
their
detection
wastewater.
Clinical
has
mostly
used
culturing,
antibiotic
susceptibility
testing,
genotyping,
employed
PCR-based
metagenomics
alongside
culture-based
techniques.
Imported
resulting
international
travel
hospitalization
abroad
appear
frequently
contributed
similarities
between
(e.g.,
knowledge
exchange
practices,
usage
patterns,
current
landscape)
could
facilitate
collaborative
efforts
developing
implementing
population-level
screening.
Frontiers in Microbiology,
Journal Year:
2025,
Volume and Issue:
15
Published: Jan. 15, 2025
Poultry
production
accounts
for
42%
of
Cameroonian
meat
production.
However,
infectious
diseases
represent
the
main
hindrance
in
this
sector,
resulting
overuse
and
misuse
antimicrobials
that
can
contribute
to
emergence
dissemination
antimicrobial
resistance
(AMR).
This
study
aimed
evaluate
prevalence
genes
(ARGs)
conferring
carbapenems
(blaVIM-2
blaNDM
),
(fluoro)
quinolones
(qnrS,
qnrA,
qnrB),
polymyxins
(mcr1
mcr5),
macrolides
(ermA
ermB)
poultry
farm
environment.
Additionally,
examined
relationship
between
these
ARGs
biosecurity
implementation,
as
well
farmers'
knowledge,
attitudes,
practices
toward
use
(AMU)
AMR,
including
their
perception
AMR
risk.
Fecal,
drinking
water,
biofilm
samples
from
water
pipelines
were
collected
15
farms
subsequently
analyzed
by
real-time
PCR
16S
rRNA
NGS.
All
tested
positive
quinolones,
97.8%
macrolides,
64.4%
polymyxins,
11.1%
carbapenems.
Of
concern,
more
than
half
(64.4%)
showed
a
multi-drug
(MDR)
pattern
(i.e.,
≥3
classes).
Drinking
microbial
communities
significantly
differed
one
fecal
samples,
both
term
diversity
(α-diversity)
composition
(β-diversity).
Furthermore,
opportunistic
pathogens
Comamonadaceae
Sphingomonadaceae)
among
most
abundant
bacteria
biofilm.
The
level
implementation
was
intermediate,
while
knowledge
attitude
farmers
AMU
insufficient
unsuitable,
respectively.
Good
found
be
correlated
with
reduction
MDR.
provides
valuable
information
on
medically
important
Cameroon
highlights
potential
impact
human
environmental
health.
Foods,
Journal Year:
2025,
Volume and Issue:
14(7), P. 1082 - 1082
Published: March 21, 2025
Bacteriophages,
first
discovered
in
1915,
have
re-emerged
as
critical
players
microbial
ecosystems,
particularly
food
production.
Their
ability
to
lysogenize
bacterial
hosts
raises
concerns
about
their
role
the
horizontal
transfer
of
antibiotic
resistance
genes
(ARGs)
and
virulence
factors,
contributing
global
challenge
antimicrobial
resistance.
Key
studies
reveal
that
ARG-carrying
phages
are
prevalent
across
various
stages
chain,
including
soil,
vegetables,
meat,
dairy,
wastewater
associated
with
These
findings
demonstrate
potential
for
lysogenic
act
vectors
gene
dissemination,
posing
risks
public
health.
The
review
also
explores
emerging
genetic
elements,
such
phage-inducible
chromosomal
islands
agents,
further
enhance
mobility
genes.
Advancements
metagenomic
tools
improved
our
understanding
phage-mediated
transfer,
but
significant
knowledge
gaps
remain.
Future
research
should
aim
quantify
these
processes
real-world
settings
develop
strategies
mitigate
systems.
Aquaculture Research,
Journal Year:
2025,
Volume and Issue:
2025(1)
Published: Jan. 1, 2025
Background:
Klebsiella
mr‐1S,
a
newly
emerging
pathogen
that
poses
substantial
threat
to
public
health
by
inducing
severe
infectious
diseases,
has
been
detected
in
Macrobrachium
rosenbergii
for
the
first
time
our
prior
investigation.
Given
its
potential
environmental
adaptation
and
genomic
evolution,
this
study
aims
delve
into
these
aspects
better
understand
pathogenicity
evolutionary
trajectory.
Methods:
In
study,
isolates
were
subjected
routine
cultivation
tryptic
soya
broth
(TSB)
medium.
Subsequently,
DNA
of
each
isolate
was
individually
extracted
analyzed.
Prior
sequencing,
integrity
concentration
samples
meticulously
evaluated
ensure
accuracy
reliability
subsequent
sequencing
process.
Results:
The
sequence
mr‐1S
deciphered,
revealing
length
5,143,806
base
pairs
with
GC
content
54.97%.
Remarkably,
genome
encompasses
multitude
putative
mobile
genetic
elements
(MGEs),
including
43
islands
(GIs)
2
prophages.
These
confer
upon
bacterium
crucial
adaptive
attributes
such
as
resistance,
virulence,
metabolic
capabilities.
Notably,
identification
prophage‐associated
clusters
originating
from
genus
Pseudomonas
suggests
horizontal
gene
transfer
(HGT)
mechanism
mediated
phages
within
Pseudomonas,
highlighting
complex
interactions
between
different
bacterial
species.
Conclusion:
addition,
presence
two
genes
encoding
CRISPR‐Cas
proteins
indicates
existence
functional
system
bacterium.
This
finding
implies
during
history,
may
have
developed
mechanisms
evade
host
immune
recognition,
thereby
facilitating
efficient
HGT
enhancing
adaptability
survival
capabilities
diverse
environments.
Overall,
provides
novel
insights
plasticity
,
underscoring
an
aquatic
ecosystems
paving
way
future
research
on
pathogenic
control
strategies.
