Access Microbiology,
Journal Year:
2023,
Volume and Issue:
5(7)
Published: July 12, 2023
The
emergence
of
antibiotic
resistance
in
livestock,
especially
food-producing
animals,
is
major
public
health
importance
as
a
result
the
possibility
these
bacteria
entering
food
chain.
In
this
study,
genetic
characteristics
antibiotic-resistant
Escherichia
coli
and
Klebsiella
spp.
isolates
from
humans
poultry
Edo
state,
Nigeria,
were
investigated.
April
2017,
45
46
E.
obtained
urine,
clinical
wounds,
nasal
chicken
faecal
samples.
Isolates
recovered
identified
previously
described.
Species
identification
was
achieved
by
matrix-assisted
laser
desorption
ionization-time
flight
(MALDI-TOF)
mass
spectrometry
ribosomal
multilocus
sequence
typing.
Antimicrobial
susceptibility
testing
carried
out
using
Kirby-Bauer
method
for
12
antibiotics.
A
double
disc
synergy
test
used
to
screen
extended-spectrum
beta-lactamse
(ESBL)
production.
Whole
genome
sequencing
performed
strain
characterization
isolates.
Thirteen
yielded
positive
results
ESBL
phenotypic
harboured
genes.
Of
isolates,
21
human
13
resistant
at
least
one
tested
Four
genes
revealed
when
applying
tests.
include
blaCTX-M-15
blaSHV-28.
genome-based
core
gene
typing
close
relatedness
among
An
integrated
'One
Health'
surveillance
system
required
monitor
transmission
antimicrobial
Nigeria.
Antibiotics,
Journal Year:
2024,
Volume and Issue:
13(6), P. 543 - 543
Published: June 11, 2024
Escherichia
coli
is
an
indicator
micro-organism
in
One
Health
antibiotic
resistance
surveillance
programs.
The
purpose
of
the
study
was
to
describe
and
compare
E.
isolates
obtained
from
pigs
human
contacts
a
commercial
farm
South
Africa
using
conventional
methods
whole-genome
sequencing
(WGS).
Porcine
were
proportionally
more
resistant
phenotypically
harbored
richer
diversity
genes
as
compared
isolates.
Different
pathovars,
namely
ExPEC
(12.43%,
21/169),
ETEC
(4.14%,
7/169),
EPEC
(2.96%,
5/169),
EAEC
5/169)
STEC
(1.18%,
2/169),
detected
at
low
frequencies.
Sequence
type
complex
(STc)
10
most
prevalent
(85.51%,
59/169)
among
porcine
Six
STcs
(STc10,
STc86,
STc168,
STc206,
STc278
STc469)
shared
human–livestock
interface
according
multilocus
sequence
typing
(MLST).
Core-genome
MLST
hierarchical
clustering
(HC)
showed
that
overall
genetically
diverse,
but
some
HC2–HC200
observed.
In
conclusion,
even
though
spatiotemporal
relationship,
there
still
differences
virulence
potential,
profiles
cgMLST
HC
source
isolation.
Frontiers in Bacteriology,
Journal Year:
2024,
Volume and Issue:
3
Published: July 16, 2024
Escherichia
coli
is
found
in
diverse
environmental
niches,
including
meat
and
products,
known
for
its
significance
both
food
safety
public
health.
In
South
Africa,
whole
genomic
sequencing
(WGS)
efforts
E.
are
overwhelmingly
skewed
toward
human
isolates
with
limited
studies
conducted
on
non-human
isolates.
Therefore,
the
aim
of
this
study
was
to
use
WGS
characterise
generic
strains
isolated
from
animal
specimens,
meat,
meat-based
products
Africa.
Based
analysis,
a
total
35
were
grouped
into
five
phylogroups
(A,
B1,
B2,
C,
E),
A
(46%)
being
most
predominant.
Virulence-associated
genes
identified
as
either
extra-intestinal
pathogenic
(69%)
or
intestinal
(31%)
pathotypes.
Twenty-three
different
serotypes
identified,
O101:H37
(17%),
O2:H4
O6:H5
(13%),
O64:H19
(9%)
predominant
ones.
Among
19
sequence
types
(STs),
ST1858,
ST975,
ST10
prevalent
(11%
each).
Various
virulence
genes,
antimicrobial
resistance
genetic
mobile
elements
carrying
Tn2,
IS26,
Tn6196
detected,
disinfectant
sitABCD
The
type
1
CRISPR
system
which
functions
by
storing
records
previous
invasions
provide
immunological
memory
rapid
robust
response
upon
subsequent
viral
infections
detected
all
isolates,
consisting
subtypes
I-E
(86%),
I-A
(57%),
I-F
(11%).
findings
an
insight
diversity
species,
Access Microbiology,
Journal Year:
2023,
Volume and Issue:
5(7)
Published: July 12, 2023
The
emergence
of
antibiotic
resistance
in
livestock,
especially
food-producing
animals,
is
major
public
health
importance
as
a
result
the
possibility
these
bacteria
entering
food
chain.
In
this
study,
genetic
characteristics
antibiotic-resistant
Escherichia
coli
and
Klebsiella
spp.
isolates
from
humans
poultry
Edo
state,
Nigeria,
were
investigated.
April
2017,
45
46
E.
obtained
urine,
clinical
wounds,
nasal
chicken
faecal
samples.
Isolates
recovered
identified
previously
described.
Species
identification
was
achieved
by
matrix-assisted
laser
desorption
ionization-time
flight
(MALDI-TOF)
mass
spectrometry
ribosomal
multilocus
sequence
typing.
Antimicrobial
susceptibility
testing
carried
out
using
Kirby-Bauer
method
for
12
antibiotics.
A
double
disc
synergy
test
used
to
screen
extended-spectrum
beta-lactamse
(ESBL)
production.
Whole
genome
sequencing
performed
strain
characterization
isolates.
Thirteen
yielded
positive
results
ESBL
phenotypic
harboured
genes.
Of
isolates,
21
human
13
resistant
at
least
one
tested
Four
genes
revealed
when
applying
tests.
include
blaCTX-M-15
blaSHV-28.
genome-based
core
gene
typing
close
relatedness
among
An
integrated
'One
Health'
surveillance
system
required
monitor
transmission
antimicrobial
Nigeria.
