A novel RofA-family transcriptional regulator, GadR, controls the development of acid resistance inListeria monocytogenes DOI Creative Commons
Jialun Wu, Olivia McAuliffe, Conor P. O’Byrne

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 22, 2023

Abstract Stomach acid provides a significant innate barrier to the entry of food-borne pathogen Listeria monocytogenes into human gastrointestinal tract. A key determinant resistance in this bacterium is conserved glutamate decarboxylase system, GadD2 (encoded by gadT2D2 operon), which helps maintain intracellular pH during exposure gastric acid. In study, we identified premature stop codon gene located immediately downstream operon that was highly linked an sensitive phenotype. When open reading frame restored through homologous recombination resistant phenotype resulted. Through series genetic, transcriptomic and survival experiments established gene, designated gadR , encodes transcriptional regulator operon. GadR belongs RofA family regulators, primarily found streptococci, where they are involved regulating virulence. The data further showed plays critical role development response mild exposure, known as adaptive tolerance (ATR). deletion analysis promoter region two 18bp palindromic sequences required for GadR-mediated induction suggesting act binding sites GadR. Overall, study uncovers new RofA-like L. both growth phase-dependent ATR mediated accounts previously observed strain-to-strain differences at low pH. findings have important implications understanding behavior acidic environments identify potential target improved control pathogen. Author summary ability survive conditions stomach trait enabling gain access mammalian tract, it can initiate infection. Little currently about how regulated why variable between strains. Here used genomic from collection strains with novel controlling resistance, call regulators only small group bacterial pathogens including virulence properties. We show dominant variability its sequence trait. Together these significantly advance our copes stress suggests molecular better food-chain.

Language: Английский

The Glutamate Decarboxylase System GAD2 is Activated by Osmotic Stress in a GadR-Dependent Manner in Listeria Monocytogenes DOI
Chuhan Wang, Jialun Wu, Conor P. O’Byrne

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Effects of the histone-like protein on biofilm formation and pathogenicity of Listeria monocytogenes DOI
Wenqian Li, Can Huang, Wei Yuan

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140908 - 140908

Published: Feb. 1, 2025

Language: Английский

Citations

0
Functional analysis of the CRP and TreR-treBC regulon in trehalose utilization of Vibrio parahaemolyticus DOI
Peng Liu, Ting Ji,

Youkun Zhang

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: 296, P. 128138 - 128138

Published: March 10, 2025

Language: Английский

Citations

0
Effect of baicalin on eradicating biofilms of bovine milk derived Acinetobacter lwoffii DOI Creative Commons

Chengjun Ma,

Mei Cui, Jingjing Liu

et al.

BMC Veterinary Research, Journal Year: 2024, Volume and Issue: 20(1)

Published: May 20, 2024

Acinetobacter lwoffii (A.lwoffii) is a serious zoonotic pathogen that has been identified as cause of infections such meningitis, bacteremia and pneumonia. In recent years, the infection rate detection A.lwoffii increasing, especially in breeding industry. Due to presence biofilms, it difficult eradicate become potential super drug-resistant bacteria. Therefore, eradication preformed biofilm an alternative therapeutic action control infection. The present study aimed clarify baicalin could dairy cows, explore mechanism eradicating A.lwoffii.

Language: Английский

Citations

3
The impact of different acidic conditions and food substrates on Listeria monocytogenes biofilms development and removal using nanoencapsulated carvacrol DOI Creative Commons
Jina Yammine, Agapi I. Doulgeraki, Conor P. O’Byrne

et al.

International Journal of Food Microbiology, Journal Year: 2024, Volume and Issue: 416, P. 110676 - 110676

Published: March 12, 2024

Listeria monocytogenes biofilms present a significant challenge in the food industry. This study explores impact of different acidic conditions culture media and matrices on development removal developed stainless steel surfaces by wild-type (WT) L. strains as well two mutant derivatives, ΔsigB ΔagrA, that have defects general stress response quorum sensing, respectively. Additionally, investigates efficacy nanoencapsulated carvacrol an antimicrobial against Tryptic Soy Broth (TSB) acidified to pH (3.5, 4.5, 5.5, 6.5), substrates (apple juice, strained yogurt, vegetable soup, semi-skimmed milk) having same levels. No biofilm formation was observed for all at levels 3.5 4.5 both substrates. However, 5.5 6.5, increased were substrates, with WT strain showing significantly higher (3.04–6.05 log CFU cm−2) than (2.30–5.48 cm−2). For applications, demonstrated more potent activity 2.23 3.61 reductions, compared 1.58–2.95 reductions mutants being vulnerable environments. In induced lower (1.58–2.90) ones TSB (2.02–3.61). These findings provide valuable insights into potential application control agent processing

Language: Английский

Citations

2
Methods for studying microbial acid stress responses: from molecules to populations DOI Creative Commons
Merve Atasoy, Simona Bartkova,

Zeynep Çetecioğlu-Gürol

et al.

FEMS Microbiology Reviews, Journal Year: 2024, Volume and Issue: 48(5)

Published: May 17, 2024

The study of how micro-organisms detect and respond to different stresses has a long history producing fundamental biological insights while being simultaneously significance in many applied microbiological fields including infection, food drink manufacture, industrial environmental biotechnology. This is well-illustrated by the large body work on acid stress. Numerous methods have been used understand impacts low pH growth survival micro-organisms, ranging from studies single cells heterogeneous populations, molecular or biophysical computational, well-understood model organisms poorly defined complex microbial consortia. Much be gained an increased general awareness these methods, so present review looks at examples that resistance, tolerance, stress responses, they can lead to, as well some problems involved using them. We hope this will interest both within beyond research community.

Language: Английский

Citations

2
Metabolic reprogramming in the food-borne pathogen Listeria monocytogenes as a critical defence against acid stress DOI Creative Commons
Jialun Wu, Chuhan Wang, Conor P. O’Byrne

et al.

FEMS Microbiology Letters, Journal Year: 2024, Volume and Issue: 371

Published: Jan. 1, 2024

Abstract The ability to sense and respond effectively acidic stress is important for microorganisms survive proliferate in fluctuating environments. As specific metabolic activities can serve buffer the cytoplasmic pH, rewire their metabolism favour these reactions thereby mitigate acid stress. orally acquired pathogen Listeria monocytogenes exploits alternative overcome encountered human stomach or food products. In this minireview, we discuss processes L. that stress, with an emphasis on proton-depleting reactions, including glutamate decarboxylation, arginine/agmatine deimination, fermentative acetoin production. We also summarize recent findings regulatory mechanisms control expression of genes are responsible activities, general response regulator SigB, arginine repressor ArgR, recently discovered RofA-like transcriptional GadR. further importance reprogramming context products within host. Finally, highlight some outstanding challenges field, understanding acid-sensing mechanisms, role intraspecies heterogeneity resistance, how a fundamental be exploited formulation improve safety reduce waste.

Language: Английский

Citations

2
The prebiotic effects of fructooligosaccharides enhance the growth characteristics of Staphylococcus epidermidis and enhance the inhibition of Staphylococcus aureus biofilm formation DOI
Li Shao, Tao Li, Suzhen Yang

et al.

International Journal of Cosmetic Science, Journal Year: 2024, Volume and Issue: 47(1), P. 155 - 167

Published: Sept. 9, 2024

Oligosaccharides have been shown to enhance the production of short chain fatty acids (SCFAs) by gut probiotics and regulate microbiota, improve intestinal health. Recent research indicates that oligosaccharides may also positively impact skin microbiota selectively promoting growth commensal bacteria inhibiting pathogenic bacteria. However, specific metabolic regulatory mechanisms in response remain unclear. This study aims explore influence four on metabolism Staphylococcus epidermidis further identify prebiotics can its probiotic effects skin. Fructooligosaccharides (FOS), isomaltooligosaccharide (IMO), galactooligosaccharides (GOS) inulin were compared terms their cell proliferation, SCFAs S. CCSM0287 biofilm inhibition effect fermentation supernatants aureus CCSM0424. Furthermore, FOS was analysed transcriptome analysis. All effectively promoted cells, increased SCFAs, with demonstrating most significant effect. Analysis indicated predominantly employs produce acetic acid isovaleric acid, differing from produced microbiota. Among oligosaccharides, addition 2% supernatant significantly inhibited CCSM0424 formation. RNA sequencing revealed 162 differentially expressed genes (84 upregulated 78 downregulated) upon treatment glucose treatment. Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis highlighted differences amino synthesis pathway, particularly arginine biosynthesis. promotes increases SCFA formation, suggesting serves as a potential prebiotic for strain CCSM0287.

Language: Английский

Citations

2
Manganese uptake mediated by the NRAMP-type transporter MntH is required for acid tolerance in Listeria monocytogenes DOI Creative Commons
Jialun Wu, Olivia McAuliffe, Conor P. O’Byrne

et al.

International Journal of Food Microbiology, Journal Year: 2023, Volume and Issue: 399, P. 110238 - 110238

Published: May 2, 2023

Language: Английский

Citations

6
The nematode Caenorhabditis elegans enhances tolerance to landfill leachate stress by increasing trehalose synthesis DOI Creative Commons

Yuru Chen,

Binsong Jin,

Jie Yu

et al.

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e17332 - e17332

Published: May 22, 2024

The burgeoning issue of landfill leachate, exacerbated by urbanization, necessitates evaluating its biological impact, traditionally overshadowed physical and chemical assessments. This study harnesses Caenorhabditis elegans , a model organism, to elucidate the physiological toxicity leachate subjected different treatment processes: nanofiltration reverse osmosis tail water (NFRO), membrane bioreactor (MBR), raw (RAW). Our investigation focuses on modulation sugar metabolism, particularly trehalose—a disaccharide serving dual functions as an energy source anti-adversity molecule in invertebrates. Upon exposure, C. showcased 60–70% reduction glucose glycogen levels alongside significant trehalose increase, highlighting adaptive response environmental stress augmenting synthesis. Notably, trehalose-related genes NFRO group were up-regulated, contrasting with MBR RAW groups, where synthesis outpaced decomposition 20–30 times. These findings suggest that predominantly counters leachate-induced through accumulation. research not only provides insights into differential impact methods but also proposes molecular framework for assessing repercussions contributing development novel strategies pollution mitigation preservation.

Language: Английский

Citations

1