The Global Secondary Metabolite Regulator AcLaeA Modulates Aspergillus carbonarius Virulence, Ochratoxin Biosynthesis, and the Mode of Action of Biopesticides and Essential Oils DOI Creative Commons

M. K. Iliadi,

Maria Varveri, Anastasia E. Kapetanakou

et al.

Toxins, Journal Year: 2024, Volume and Issue: 17(1), P. 2 - 2

Published: Dec. 24, 2024

Aspergillus carbonarius is considered one of the main fungi responsible for black and sour rot in grapes, as well production carcinogenic mycotoxin ochratoxin A. The global regulatory methyltransferase protein LaeA controls various secondary metabolites species, influences sexual asexual reproduction morphology. goal this study was to investigate role gene AclaeA physiology, virulence, A (OTA) by deleting from genome a wild-type strain. evaluation data on morphological characteristics, virulence experiments three different grape varieties, OTA analysis ΔAclaeA mutants showed that growth strains were significantly reduced. mutant also less virulent, producing 40-50% conidia cultivars berries. Additionally, considerably repressed after application commercial biopesticides (Trianum-P®, Vacciplant®, Serenade® Max) essential oils (EOs) cinnamon, geranium, thyme, which shown inhibit biosynthesis carbonarius. can contribute broader understanding during carbonarius-grape interactions, discovery mode action biological plant protection products EOs against mycotoxigenic fungus.

Language: Английский

A Comprehensive Review of Mycotoxins, Their Toxicity, and Innovative Detoxification Methods DOI Creative Commons
Ravikant Shekhar, Vinay B. Raghavendra,

P. Rachitha

et al.

Toxicology Reports, Journal Year: 2025, Volume and Issue: 14, P. 101952 - 101952

Published: Feb. 7, 2025

A comprehensive overview of food mycotoxins, their toxicity, and contemporary detoxification techniques is given in this article. Mycotoxins, which are harmful secondary metabolites generated by a variety fungi, including Fusarium, Aspergillus, Penicillium, provide serious health concerns to humans animals. These include hepatotoxicity, neurotoxicity, carcinogenicity. Mycotoxins commonly found basic products, as evidenced recent studies, raising worries about public safety. The article discusses detection such enzyme-linked immunosorbent assays (ELISA), quick strip tests. Moreover, the use various control systems associated with mycotoxinis highlighted. In addition, novel strategies nanotechnology, plant extracts, omics studies were also discussed. When taken whole, analysis helps clarify pressing need for efficient management monitoring prevent mycotoxin contamination chain.

Language: Английский

Citations

3
Enhanced sensitivity in electrochemical detection of ochratoxin A within food samples using ferrocene- and aptamer-tethered gold nanoparticles on disposable electrodes DOI Creative Commons
Wicem Argoubi, Faisal K. Algethami, Noureddine Raouafi

et al.

RSC Advances, Journal Year: 2024, Volume and Issue: 14(12), P. 8007 - 8015

Published: Jan. 1, 2024

Ensuring food security is crucial for public health, and the presence of mycotoxins, produced by fungi in improperly stored processed or unprocessed food, poses a significant threat. This research introduces novel approach - disposable aptasensing platform designed detection ochratoxin A (OTA). The employs gold-nanostructured screen-printed carbon electrodes functionalized with ferrocene derivative, serving as an integrated faradaic transducing system, anti-OTA aptamer bioreceptor site. Detection relies on electrochemical signal changes induced folding target molecule. Remarkably sensitive, detects OTA within range 0.5 to 70 ng mL-1 limit 11 pg mL-1. approximately 200 times below levels stipulated European Commission agricultural commodities. Notably, sensing device exhibits efficacy detecting complex media, such roasted coffee beans wine, without need sample pretreatment, yielding accurate recoveries. Furthermore, while label-free aptasensors have proliferated, this study addresses gap understanding binding mechanisms some aptasensors. To enhance experimental findings, theoretical was conducted underscore specificity donor detection. molecular docking technique employed unveil key region aptamer, providing valuable insights into aptasensor specificity.

Language: Английский

Citations

8
Polyphenols from Common Beans (Phaseolus vulgaris L.) with Antimycotoxigenic Potential against Fumonisin B1 DOI
María Viñas, Andrea Irías-Mata, Tania Chacón-Ordóñez

et al.

ACS Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Language: Английский

Citations

1
An overview of the antifungal potential for aromatic plant extracts in agriculture and the food industry: A comprehensive analysis focusing on the Rubus, Cistus and Quercus genera against fungal infections of crops and food DOI Creative Commons

María Ángeles Rivas,

Rocío Casquete, Maria Teresa Santos

et al.

International Journal of Food Microbiology, Journal Year: 2025, Volume and Issue: 436, P. 111209 - 111209

Published: April 12, 2025

Language: Английский

Citations

0
Biocontrol Agents and Natural Feed Supplements as a Safe and Cost-Effective Way for Preventing Health Ailments Provoked by Mycotoxins DOI Creative Commons
S. Stoev

Foods, Journal Year: 2025, Volume and Issue: 14(11), P. 1960 - 1960

Published: May 31, 2025

The relationships between mycotoxins content in food commodities or feedstuffs and the foodborne diseases is well known. So far, available data mainly include chemical methods of decontamination for agricultural raw materials, including mycotoxin binders. Therefore, possible use some natural cost-effective supplements such as herbs, fungi, microorganisms, plants with powerful safe protection against mycotoxin-induced health ailments main subject this review paper. Various antagonistic microorganisms yeast fungicidal properties, herbs that suppress fungal development subsequent production target and/or have protective effect mycotoxins, are deeply studied literature, practical suggestions given regard. by degradation, biotransformation, binding using additives to foods has also been thoroughly investigated analyzed a approach ameliorating adverse effects mycotoxins. Possible beneficial dietary changes potentially alleviate toxicity. Practical advice provided application same real-life practice combating ailments. Natural feed bioactive compounds appeared be emerging approaches preventing caused However, address vitro studies, more vivo experiments necessary introducing industry. Generally, herbal supplements, antioxidants, polyenzyme complements could used protectors addition Bioactive agents enzymatic degradation reported very successful regard PAT OTA, whereas microorganisms/fungi/yeasts AFs PAT-producing fungi.

Language: Английский

Citations

0
Inhibitory Effects and Mechanisms of Perilla Essential Oil and Perillaldehyde against Chestnut Pathogen Botryosphaeria dothidea DOI Creative Commons

Qi Zeng,

Lu Wang,

Long Sha

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(8), P. 526 - 526

Published: July 28, 2024

Botryosphaeria dothidea, a notorious plant pathogen, is responsible for causing chestnut rot during postharvest storage. This research aimed to assess the antifungal properties of perilla essential oil (PEO) and perillaldehyde (PAE) against B. dothidea. PEO’s PAE’s inhibitory effects on dothidea were investigated using an agar dilution method, fumigation in vivo assay chestnuts shell buckets. Based results gas chromatography-mass spectrometry, it was confirmed that main component PEO elemicin. The mechanism PAE by conducting staining experiments fungal cell wall membrane. strongly inhibit mycelial growth dose-dependent manner. mainly related destruction integrity plasma Notably, retains its efficacy chestnuts, effectively prolonging their storage life. These findings indicate are nontoxic, eco-friendly botanical fungicides, holding promise controlling rot.

Language: Английский

Citations

2
Anti-aflatoxin potential of phenolic compounds from common beans (Phaseolus vulgaris L.) DOI

Dayana León-Cortés,

Kelvin Arce-Villalobos,

Diego Bogantes-Ledezma

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 469, P. 142597 - 142597

Published: Dec. 24, 2024

Language: Английский

Citations

2
Chitosan as an Antimicrobial, Anti-Insect, and Growth-Promoting Agent for Potato (Solanum tuberosum L.) Plants DOI Creative Commons
Aleksandra Steglińska, Adriana Nowak, R. Janas

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(14), P. 3313 - 3313

Published: July 13, 2024

A growing trend in plant protection is replacing chemical preparations with environmentally friendly biological compositions. Chitosan, due to its biocompatibility, biodegradability, and bioactivity, an effective agent against diseases. The purpose of the study was evaluate chitosan as a potential biopesticide for potato plants. Three variants were tested: high (310–375 kDa, >75% deacetylated), medium (190–310 75–85% low (50–190 deacetylated) molecular weight. dissolved lactic succinic acids tested antibacterial antifungal properties eight strains mould two bacteria responsible possible cytotoxicity evaluated different cell lines: insect Sf-9, human keratinocyte HaCaT, colon carcinoma Caco-2. bioprotective activities also situ on tubers. Chitosan inhibited growth almost all selected phytopathogens. most active acid. This formula characterized by toxicity towards cells Sf-9 cells. It found have positive effects stems roots, gas exchange, chlorophyll index Selected formulation proposed functional

Language: Английский

Citations

1
Application and antagonistic mechanisms of atoxigenic Aspergillus strains for the management of fungal plant diseases DOI

Suyan Wang,

Yanxia Wang,

Xin‐Chi Shi

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

ABSTRACT This review covers, for the first time, all methods based on use of Aspergillus strains as biocontrol agents management plant diseases caused by fungi and oomycetes. Atoxigenic have been screened in a variety hosts, such peanuts, maize kernels, legumes, during preharvest postharvest stages. These against wide range pathogens, Fusarium , Phytophthora Pythium species, suggesting broad applicability spectrum. The highest efficacies were generally observed when using non-toxigenic mycotoxin-producing strains. modes action included synthesis antifungal metabolites, kojic acid volatile organic compounds (VOCs), secretion hydrolytic enzymes, competition space nutrients, induction disease resistance. degraded Sclerotinia sclerotiorum sclerotia, showing high control efficacy this pathogen. Collectively, although two commercialized aflatoxin degradation, new application is emerging needs to be optimized.

Language: Английский

Citations

1
The Global Secondary Metabolite Regulator AcLaeA Modulates Aspergillus carbonarius Virulence, Ochratoxin Biosynthesis, and the Mode of Action of Biopesticides and Essential Oils DOI Creative Commons

M. K. Iliadi,

Maria Varveri, Anastasia E. Kapetanakou

et al.

Toxins, Journal Year: 2024, Volume and Issue: 17(1), P. 2 - 2

Published: Dec. 24, 2024

Aspergillus carbonarius is considered one of the main fungi responsible for black and sour rot in grapes, as well production carcinogenic mycotoxin ochratoxin A. The global regulatory methyltransferase protein LaeA controls various secondary metabolites species, influences sexual asexual reproduction morphology. goal this study was to investigate role gene AclaeA physiology, virulence, A (OTA) by deleting from genome a wild-type strain. evaluation data on morphological characteristics, virulence experiments three different grape varieties, OTA analysis ΔAclaeA mutants showed that growth strains were significantly reduced. mutant also less virulent, producing 40-50% conidia cultivars berries. Additionally, considerably repressed after application commercial biopesticides (Trianum-P®, Vacciplant®, Serenade® Max) essential oils (EOs) cinnamon, geranium, thyme, which shown inhibit biosynthesis carbonarius. can contribute broader understanding during carbonarius-grape interactions, discovery mode action biological plant protection products EOs against mycotoxigenic fungus.

Language: Английский

Citations

0