Transcriptome Sequencing Reveals Survival Strategies and Pathogenic Potential of Vibrio parahaemolyticus Under Gastric Acid Stress DOI Creative Commons
Shiqi Ji, Jinlin Jiang, Zhiyong Song

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(4), P. 396 - 396

Published: April 10, 2025

As a common food-borne pathogen, Vibrio parahaemolyticus comes into direct or indirect contact with gastric acid after ingestion. However, the mechanisms by which passes through barrier, recovers, and causes pathogenicity remain unclear. In this study, static in vitro digestion simulation experiments showed that some strains can pass barrier utilizing microacid tolerance altering their survival state. Food food matrices could help bacteria escape stress, significantly different rates observed for various exposure to acid. Interestingly, surviving shorter growth lag time (LT) during recovery. Transcriptome sequencing (RNA-seq) analyses indicated adapted stress regulating two-component system proteins secreted via ribosomal pathway. Pathogenic successfully potentially exhibits enhanced recovery due significant upregulation of virulence genes such as tdh yscF. This study provides scientific basis revealing pathogens represented human body.

Language: Английский

Proteomic Analysis Reveals the Phenotypic Heterogeneity and Tolerance Mechanisms of Halophilic Vibrio parahaemolyticus Under Dual Stress of Low Salinity and Bile Salts in the Human Intestine DOI Creative Commons
Yingying Guo, Bing Yang, Xiaoyan Zhou

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(4), P. 518 - 518

Published: April 1, 2025

Vibrio parahaemolyticus, a halophilic Gram-negative bacterium commonly found in aquatic products, can colonize the human small intestine, causing gastroenteritis and potentially leukemia. As major intestinal pathogen, it poses significant threat to public health. This study aims investigate phenotypic heterogeneity of V. parahaemolyticus low-salinity bile salt environments tract elucidate its mechanisms tolerance pathogenicity using proteomics. The experimental results indicated that under low salinity salts conditions environment, growth, motility, biofilm formation strains were significantly inhibited. Proteomics analysis revealed that, these conditions, energy metabolism, chemotaxis system, flagellar motor, ribosome-related proteins affected, thereby influencing formation. Furthermore, activation secretion particularly T2SS, enhanced virulence secreted factors on host cells. Additionally, β-lactam resistance pathway increased enhancing parahaemolyticus.

Language: Английский

Citations

0
Transcriptome Sequencing Reveals Survival Strategies and Pathogenic Potential of Vibrio parahaemolyticus Under Gastric Acid Stress DOI Creative Commons
Shiqi Ji, Jinlin Jiang, Zhiyong Song

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(4), P. 396 - 396

Published: April 10, 2025

As a common food-borne pathogen, Vibrio parahaemolyticus comes into direct or indirect contact with gastric acid after ingestion. However, the mechanisms by which passes through barrier, recovers, and causes pathogenicity remain unclear. In this study, static in vitro digestion simulation experiments showed that some strains can pass barrier utilizing microacid tolerance altering their survival state. Food food matrices could help bacteria escape stress, significantly different rates observed for various exposure to acid. Interestingly, surviving shorter growth lag time (LT) during recovery. Transcriptome sequencing (RNA-seq) analyses indicated adapted stress regulating two-component system proteins secreted via ribosomal pathway. Pathogenic successfully potentially exhibits enhanced recovery due significant upregulation of virulence genes such as tdh yscF. This study provides scientific basis revealing pathogens represented human body.

Language: Английский

Citations

0