A novel Erwiniaceae gut symbiont modulates gene expression of the intracellular bacterium Cardinium in the stored product mite Tyrophagus putrescentiae

mSphere, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

ABSTRACT We examined host and bacterial gene expression profiles in the stored product mite Tyrophagus putrescentiae co-infected with Wolbachia (wTPut) Cardinium (cTPut) while varying presence of Erwiniaceae symbiont (SLS). SLS, a novel family , genome size 1.7 Mb, is found 16% species infected cultures. In addition, SLS was detected feces but not their eggs. Although remained unchanged, or absence significantly affected expression. It indicated that effect on neutral. SLS-positive samples, exhibited 29 upregulated 48 downregulated genes compared to SLS-negative samples. Furthermore, strongly correlated KEGG Positive Spearman’s correlations between immune regulatory pathways were doubled The diversity expressed decreased SLS. had more interacting samples than without Transposases are most genes, showing upregulation Correlation analyses revealed interactions via pathways, including lysosome, ubiquitin-mediated proteolysis, PIK3_Akt, cGMP-PKG. results showed indirectly affects gut symbionts mites. IMPORTANCE This study introduces new model analyze intracellular symbionts, hosts. Using correlations, we investigated how responds mold . data both different symbionts. increased interaction terms changes pathway differently relation As well-known producer allergens, T. physiology thus its allergen production influenced by potentially affecting release allergens into human environments.

Language: Английский

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Cardinium disrupts Wolbachia –host dynamics in the domestic mite Tyrophagus putrescentiae : evidence from manipulative experiments

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

ABSTRACT We investigated the tripartite interactions between two intracellular bacterial symbionts, Cardinium and Wolbachia in Tyrophagus putrescentiae . Cultures of are typically single-infected by one symbiont. However, co-infection can be experimentally induced mixing cultures, resulting 10% mite individuals being double-infected ( + ) a corresponding reduction host fitness. Here, we assembled genomes analyzed their gene expression parental mixed cultures using population-level samples (ranging from 7,500 to 10,000 mites). interacts more extensively with its than cultures. (i) exhibited reduced regulation compared ; (ii) profile shifted, increasing host, whereas remained unchanged; (iii) genes loss expression, as indicated correlations (for example MAPK, endocytosis, calcium signaling pathways). The experiments show that at population level, symbiont infection disrupts interaction symbionts different ways. was influenced vice versa. inhibit growth disrupting leading Wolbachia’s influence on immune regulatory pathways. reasons for responses due or frequency analyses samples. IMPORTANCE found host. In strong exist expressions. Interestingly, although also interact this appears weaker These results suggest both affect particularly samples, outcompete interaction. It indicates competition these populations. belongs mite-specific supergroup Q, distinct commonly studied supergroups. As bacteria exhibit pathogen-blocking effects, our findings may have relevance other systems, such ticks tick-borne diseases. study sheds light within novel mite-symbiont model.

Language: Английский

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Cultures of stored-product mite Tyrophagus putrescentiae experimentally infected with Cardinium and Wolbachia presented reduced fitness

Journal of Stored Products Research, Journal Year: 2025, Volume and Issue: 112, P. 102663 - 102663

Published: April 23, 2025

Language: Английский

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Molecular evidence of Wolbachia in bat-associated mite Periglischrus Iheringi Oudemans, 1902 (Mesostigmata: Spinturnicidae) from Brazil

Veterinary Research Communications, Journal Year: 2024, Volume and Issue: 49(1)

Published: Dec. 30, 2024

Language: Английский

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