Light-Promoted Lysosomal Escape of a Phthalocyanine and Antisense Oligonucleotide-Complexed G-Quadruplex for Dual Photodynamic and Antisense Therapy DOI Creative Commons
Dick Yan Tam,

Wendy K. M. Lau,

Yosephine Tania Limanto

et al.

ACS Pharmacology & Translational Science, Journal Year: 2024, Volume and Issue: 7(10), P. 3216 - 3227

Published: Sept. 25, 2024

Combination therapy has been proven as an effective strategy for cancer treatment. To this end, we report herein a self-assembled nucleic acid-based complex dual photodynamic and antisense therapy. It contains nucleolin-targeting As1411-based G-quadruplex platform, partially hybridized oligonucleotide 4625, which can inhibit the antiapoptotic protein B cell lymphoma-xL inducing apoptotic death, zinc(II) phthalocyanine (ZnPc)-based photosensitizer held by noncovalent interactions. Through series of in vitro experiments, have demonstrated that DNA be internalized selectively to nucleolin-overexpressed MCF-7 A549 cells through receptor-mediated endocytosis is localized lysosomes. Upon light irradiation, photosensitization ZnPc triggers formation reactive oxygen species killing promotes lysosomal escape 4625 The combined therapeutic effect eliminate effectively with half maximal inhibitory concentration ca. 0.5 μM.

Language: Английский

One-pot modular nanogel platform utilizing phenolic gelatin-polyethyleneimine for small RNA delivery DOI
Kyoungmin Park, Jie Zheng,

Jeong-Eun Yoo

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159532 - 159532

Published: Jan. 1, 2025

Language: Английский

Citations

0
Flavonoid-Based Nanogels: A Comprehensive Overview DOI Creative Commons
Sergio Liga, Cristina Paul

Gels, Journal Year: 2025, Volume and Issue: 11(4), P. 267 - 267

Published: April 4, 2025

The growing field of nanotechnology has recently given much attention to nanogels, which are versatile formulas and have promising biomedical applications. Nanogels or nanohydrogels undergone significant development in various fields industrial research meet increasing demands, such as pharmaceuticals, cosmetics, food, genetic engineering. that contain flavonoids, secondary metabolites found plants, starting become distinctive reveal their unique characteristics. objective the article is provide a comprehensive overview recent articles on flavonoid-based emphasizing general aspects regarding nanogel formulation structural characterization, well advancements made field. In conclusion, this outlines up-to-date developments synthesis, formulation, therapeutic applications important role nanotechnology.

Language: Английский

Citations

0
Nanogels—Innovative Drug Carriers for Overcoming Biological Membranes DOI Creative Commons
Lyubomira Radeva, Krassimira Yoncheva

Gels, Journal Year: 2025, Volume and Issue: 11(2), P. 124 - 124

Published: Feb. 8, 2025

Nanogels are promising drug delivery systems since they possess undeniable advantages such as high loading capacity for hydrophilic and hydrophobic drugs, stabilization of sensitive biocompatibility, biodegradability. The present review summarizes experimental studies related to carriers, loading, membrane transport nanogels. In particular, the discusses properties, advantages, limitations polymeric carriers with respect behavior prepared nanogels in vivo conditions. potential nanogel encapsulation or drugs mechanisms release also emphasized. Moreover, challenges through barriers presented parenteral, oral, ocular, nasal, dermal routes administration considered.

Language: Английский

Citations

0
Chitosan-Tricarbocyanine-Based Nanogels Were Able to Cross the Blood–Brain Barrier Showing Its Potential as a Targeted Site Delivery Agent DOI Creative Commons

Emilio Rivera López,

Cecilia Samaniego López, Carla Spagnuolo

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(7), P. 964 - 964

Published: July 21, 2024

Targeting drugs to the central nervous system (CNS) is challenging due presence of blood-brain barrier (BBB). The cutting edge in nanotechnology generates optimism overcome growing challenges biomedical sciences through effective engineering nanogels. primary objective present report was develop and characterize a biocompatible natural chitosan (CS)-based NG that can be tracked thanks tricarbocyanine (CNN) fluorescent probe addition on biopolymer backbone. FTIR shed light chemical groups involved CS CNN interactions between CNN-CS tripolyphosphate, cross-linking agent. Both vitro vivo experiments were carried out determine if CS-NGs utilized as therapeutic delivery vehicles directed towards brain. An ionic gelation method chosen generate cationic CNN-CS-NG. DLS TEM confirmed these entities' sizes fell into nanoscale. CNN-CS-NG found non-cytotoxic, determined SH-SY5Y neuroblastoma cell line biocompatibility assays. After cellular internalization, occurrence an endo-lysosomal escape (a crucial event for efficient drug delivery) detected. Furthermore, administered intraperitoneally female CF-1 mice detected different brain regions after 2 h administration, using fluorescence microscopy. To conclude, obtained findings useful field neuro-nanomedicine when designing with purpose delivering CNS.

Language: Английский

Citations

2
Core–Shell Nanogels With Raspberry Architecture and Amine Loading in the Core via Precipitation Polymerization: A Mechanistic Study DOI Creative Commons
Bruno Espuche, Krishan Kumar, Paolo Moretti

et al.

Macromolecules, Journal Year: 2024, Volume and Issue: 57(13), P. 6035 - 6048

Published: June 11, 2024

Nanogels (NGs) are synthesized by precipitation polymerization of dendritic polyglycerol (dPG), N-isopropylacrylamide (NIPAM), and N-isopropyl methacrylamide (NIPMAM). The stabilization agglomeration subunits during the NG growth result in raspberry-like structures, as shown transmission electron microscopy, atomic force small-angle X-ray scattering measurements. Positive charges introduced into dPG-NIPAM-NIPMAM NGs (1) copolymerization dimethylaminoethyl methacrylate (DMAEMA) (2) glycidyl (GMA), followed its functionalization with ethylenediamine (ED) through epoxy group. Homogeneous structures obtained batch DMAEMA other monomers, whereas core–shell reached semibatch GMA. After amination, restricted to core NGs.

Language: Английский

Citations

1
Light-Promoted Lysosomal Escape of a Phthalocyanine and Antisense Oligonucleotide-Complexed G-Quadruplex for Dual Photodynamic and Antisense Therapy DOI Creative Commons
Dick Yan Tam,

Wendy K. M. Lau,

Yosephine Tania Limanto

et al.

ACS Pharmacology & Translational Science, Journal Year: 2024, Volume and Issue: 7(10), P. 3216 - 3227

Published: Sept. 25, 2024

Combination therapy has been proven as an effective strategy for cancer treatment. To this end, we report herein a self-assembled nucleic acid-based complex dual photodynamic and antisense therapy. It contains nucleolin-targeting As1411-based G-quadruplex platform, partially hybridized oligonucleotide 4625, which can inhibit the antiapoptotic protein B cell lymphoma-xL inducing apoptotic death, zinc(II) phthalocyanine (ZnPc)-based photosensitizer held by noncovalent interactions. Through series of in vitro experiments, have demonstrated that DNA be internalized selectively to nucleolin-overexpressed MCF-7 A549 cells through receptor-mediated endocytosis is localized lysosomes. Upon light irradiation, photosensitization ZnPc triggers formation reactive oxygen species killing promotes lysosomal escape 4625 The combined therapeutic effect eliminate effectively with half maximal inhibitory concentration ca. 0.5 μM.

Language: Английский

Citations

0