Exploring glutathione-decorated micelles for drug delivery: a promise for enhanced cellular uptake DOI Creative Commons

Martyna Truszkowska,

Elsa Reisenberger,

Gergely Kali

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: unknown, P. 114664 - 114664

Published: March 1, 2025

This study aimed to evaluate the effect of thiolated micelles on cellular uptake their payload. Reduced and oxidized glutathione were covalently attached palmitic acid via amide bond formation. Micelles formed with these surfactants (MP-GSH MP-GSSG-P) evaluated regarding critical micellar concentration (CMC) hemolytic activity. Cytotoxicity was HEK 293 HeLa cells. Diffusion in cells by fluorescence correlation spectroscopy (FCS). Furthermore, containing coumarin-6 as model drug analyzed flow cytometry confocal laser scanning microscopy. CMC MP-GSSG-P, MP-GSH, (MPA) determined be 0.455 mM, 0.166 0.046 respectively. At a 0.5 % MPA caused around 80 hemolysis. In cells, MP-GSSG-P exhibited toxicity at 0.25 %, while MP-GSH showed 0.06 after 4 hours incubation. contrast, more resilient, only showing %. Diffusivity within higher than that MPA. Cellular studies demonstrated significantly (p < 0.05) enhanced internalization 112-fold 270-fold 12-fold 60-fold when compared These findings suggest could serve promising vehicles for enhancing drugs.

Language: Английский

Exploring glutathione-decorated micelles for drug delivery: a promise for enhanced cellular uptake DOI Creative Commons

Martyna Truszkowska,

Elsa Reisenberger,

Gergely Kali

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: unknown, P. 114664 - 114664

Published: March 1, 2025

This study aimed to evaluate the effect of thiolated micelles on cellular uptake their payload. Reduced and oxidized glutathione were covalently attached palmitic acid via amide bond formation. Micelles formed with these surfactants (MP-GSH MP-GSSG-P) evaluated regarding critical micellar concentration (CMC) hemolytic activity. Cytotoxicity was HEK 293 HeLa cells. Diffusion in cells by fluorescence correlation spectroscopy (FCS). Furthermore, containing coumarin-6 as model drug analyzed flow cytometry confocal laser scanning microscopy. CMC MP-GSSG-P, MP-GSH, (MPA) determined be 0.455 mM, 0.166 0.046 respectively. At a 0.5 % MPA caused around 80 hemolysis. In cells, MP-GSSG-P exhibited toxicity at 0.25 %, while MP-GSH showed 0.06 after 4 hours incubation. contrast, more resilient, only showing %. Diffusivity within higher than that MPA. Cellular studies demonstrated significantly (p < 0.05) enhanced internalization 112-fold 270-fold 12-fold 60-fold when compared These findings suggest could serve promising vehicles for enhancing drugs.

Language: Английский

Citations

0