Innovative microfluidic model for investigating the intestinal mucus barrier: numerical and experimental perspectives DOI Creative Commons
Mohammad Valibeknejad, Reza Alizadeh, S. Majid Abdoli

et al.

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

The intestinal mucus layer serves as a critical first line of defense against external agents, functioning barrier to the absorption drugs, food, and pathogens. While numerous in vitro studies have explored role preventing particle penetration, effects flowing luminal material, dislodging because induced shear rate by lumen material interfacial phenomena remain poorly understood. This study introduces microfluidic approach simulate interaction between layer. successfully measures both penetration into dislodgement material. A biosimilar model (BSM) Hank's Balanced Salt Solution (HBSS) were employed mimics human fluid, respectively. To investigate effect viscosity on pattern, two variants used: BSM-1, representing low-viscosity model, BSM-2, high-viscosity model. velocity fields extracted tracking fluorescent particles. results revealed significant differences BSM-1 attributed their rheological properties. These findings further confirmed through an assessment viscoelastic properties BSM models. utilized COMSOL Multiphysics for numerical simulations, predicting experimental outcomes solving fluid flow equations. Physicochemical characterizations HBSS performed link with including sweep tests, application power-law viscosity, measurements density wettability. proposes platform examining low- offer valuable insights barrier's response stress. validated physicochemical provide foundation future rates more complex geometries diverse conditions.

Language: Английский

Innovative microfluidic model for investigating the intestinal mucus barrier: numerical and experimental perspectives DOI Creative Commons
Mohammad Valibeknejad, Reza Alizadeh, S. Majid Abdoli

et al.

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

The intestinal mucus layer serves as a critical first line of defense against external agents, functioning barrier to the absorption drugs, food, and pathogens. While numerous in vitro studies have explored role preventing particle penetration, effects flowing luminal material, dislodging because induced shear rate by lumen material interfacial phenomena remain poorly understood. This study introduces microfluidic approach simulate interaction between layer. successfully measures both penetration into dislodgement material. A biosimilar model (BSM) Hank's Balanced Salt Solution (HBSS) were employed mimics human fluid, respectively. To investigate effect viscosity on pattern, two variants used: BSM-1, representing low-viscosity model, BSM-2, high-viscosity model. velocity fields extracted tracking fluorescent particles. results revealed significant differences BSM-1 attributed their rheological properties. These findings further confirmed through an assessment viscoelastic properties BSM models. utilized COMSOL Multiphysics for numerical simulations, predicting experimental outcomes solving fluid flow equations. Physicochemical characterizations HBSS performed link with including sweep tests, application power-law viscosity, measurements density wettability. proposes platform examining low- offer valuable insights barrier's response stress. validated physicochemical provide foundation future rates more complex geometries diverse conditions.

Language: Английский

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