What is eDNA method standardisation and why do we need it?
Metabarcoding and Metagenomics,
Journal Year:
2025,
Volume and Issue:
9
Published: Feb. 13, 2025
The
rapid
advancement
of
environmental
DNA
(eDNA)
science
in
the
past
two
decades
has
inspired
a
concomitant
growth
development
eDNA
sampling
and
analytical
methods.
However,
these
methods
are
often
developed
by
individual
laboratories
or
institutions,
which
can
isolate
protocols
within
programmes,
agencies
regions
prevent
beneficial
exchange
data
ideas.
Recent
efforts
to
advance
national
international
coordination
have
resulted
groundswell
standardisation
efforts,
but
there
is
still
considerable
confusion
around
role
formal
standards
for
regulatory
research
applications.
With
this
commentary,
we
hope
provide
clarity
on
terminology
used
discussions,
including
differences
between
best
practice
guidelines.
Additionally,
discuss
how
method
choice
may
be
informed
management
scenarios
review
examples
being
inform
action.
community
now
an
opportunity
develop
roadmap
help
close
gaps,
adoption
accelerate
our
ability
monitor
biological
life
at
scales
current
challenges
demand.
Language: Английский
Academic institution extensive, building-by-building wastewater-based surveillance platform for SARS-CoV-2 monitoring, clinical data correlation, and potential national proxy
PLOS Global Public Health,
Journal Year:
2025,
Volume and Issue:
5(5), P. e0003756 - e0003756
Published: May 9, 2025
In
this
work,
we
report
on
the
performance
of
an
extensive,
building-by-building
wastewater
surveillance
platform
deployed
across
38
locations
largest
private
university
system
in
Mexico,
spanning
19
32
states,
to
detect
SARS-CoV-2
genetic
materials
during
COVID-19
pandemic.
Sampling
took
place
weekly
from
January
2021
and
June
2022.
Data
343
sampling
sites
was
clustered
by
campus
state
evaluated
through
its
correlation
with
seven-day
average
daily
new
cases
each
cluster.
Statistically
significant
linear
correlations
(p-values
below
0.05)
were
found
25
campuses
13
states.
Moreover,
evaluate
effectiveness
epidemiologic
containment
measures
taken
institution
potential
as
representative
points
for
future
public
health
emergencies
Monterrey
Metropolitan
Area,
between
viral
loads
samples
be
stronger
Dulces
Nombres,
treatment
plant
city
(Pearson
coefficient:
0.6456,
p-value:
6.36710
−8
),
than
study
0.4860,
8.288x10
−5
).
However,
when
comparing
data
after
urban
mobility
returned
pre-pandemic
levels,
levels
both
became
comparable
(0.894
0.865
Nombres).
This
work
provides
a
basic
framework
implementation
analysis
similar
decentralized
platforms
address
sanitary
emergencies,
allowing
efficient
return
priority
in-person
activities
while
preventing
becoming
transmission
hotspots.
Language: Английский
The potential of long‐term wastewater‐based surveillance to predict COVID‐19 waves peak in Mexico
Water Environment Research,
Journal Year:
2025,
Volume and Issue:
97(5)
Published: May 1, 2025
Abstract
Wastewater‐based
surveillance
(WBS)
is
valuable
method
for
monitoring
the
dispersion
of
pathogens
at
a
low
cost.
However,
their
impact
on
public
health
decision‐making
limited
because
there
lack
long‐term
analyses,
especially
in
low‐
and
middle‐income
countries.
This
study
aimed
to
assess
effectiveness
using
WBS
predict
occurrence
COVID‐19
waves
estimate
prevalence
infection,
emphasizing
SARS‐CoV‐2
variants.
During
17
months
influent
two
wastewater
treatment
plants
Queretaro
City,
Mexico,
wave
prediction
time
was
influenced
by
variant
dispersion.
Waves
dominated
Delta
Omicron
variants
circulation
showed
lead
days
values
from
5
14
1
4
days,
respectively.
According
Monte
Carlo
model,
disease
aligned
with
clinically
reported
cases
onsets,
but
variant's
transmissibility
explained
overestimation
during
peaks.
work
provides
new
insights
into
potential
limitations
as
an
epidemiological
tool
detecting
predicting
occurrence.
Practitioner
Points
Long‐term
allowed
early
case
waves.
The
capability
related
presence
infectivity.
estimated
higher
all
estimation
has
regarding
variations
data
input.
Language: Английский